Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

Patent · Tue Oct 18 00:00:00 EDT 1983

OSTI ID:6747531

Dolbeare, F A; Gray, J W

A method for the simultaneous flow cylometric measurement of total cellular DNA content and of the uptake of DNA precursors as a measure of DNA synthesis during various phases of the cell cycle in normal and malignant cells in vitro and in vivo is described. The method comprises reacting cells with labelled halodeoxyuridine (HdU), partially denaturing cellular DNA, adding to the reaction medium monoclonal antibodies (mabs) reactive with HdU, reacting the bound mabs with a second labelled antibody, incubating the mixture with a DNA stain, and measuring simultaneously the intensity of the DNA stain as a measure of the total cellular DNA and the HdU incorporated as a measure of DNA synthesis. (ACR)

• DOE Contract Number: W-7405-ENG-48
• Assignee: Dept. of Energy
• Patent Number(s): None
• Application Number: ON: DE84014504
• OSTI ID: 6747531
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIBODIES
ANTIMETABOLITES
AZINES
BIOSYNTHESIS
CELL CYCLE
CELL FLOW SYSTEMS
DEOXYURIDINE
DNA
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IN VITRO
IN VIVO
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MEASURING METHODS
MONOCLONAL ANTIBODIES
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
SYNTHESIS
TRACER TECHNIQUES
URACILS