Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

Patent · Thu Dec 31 23:00:00 EST 1987

OSTI ID:866752

Dolbeare, Frank A ^[1]; Gray, Joe W ^[1]

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1. Livermore, CA

A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide or Hoechst 33258 is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as halodeoxy-uridine (HdU), more specifically bromodeoxyuridine (BrdU) is used as a probe for the measurement of HdU or BrdU uptake by the cells as a measure of DNA synthesis.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA
• DOE Contract Number: W-7405-ENG-48
• Assignee: Regents of University of California (Berkeley, CA)
• Patent Number(s): US 4780406
• OSTI ID: 866752
• Country of Publication: United States
• Language: English

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