Flow cytometric measurement of total DNA and incorporated halodeoxyuridine

Patent · Wed Jan 01 00:00:00 EST 1986

OSTI ID:865829

Dolbeare, Frank A ^[1]; Gray, Joe W ^[1]

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1. Livermore, CA

A method for the simultaneous flow cytometric measurement of the total DNA content and the level of DNA synthesis in normal and malignant cells is disclosed. The sensitivity of the method allows a study of cell cycle traverse rates for large scale cell populations as well as single cell measurements. A DNA stain such as propidium iodide is used as the probe for the measurement of total DNA content and a monoclonal antibody reactive with a DNA precursor such as bromodeoxyuridine (BrdU) is used as a probe for the measurement of BrdU uptake by the cells as a measure of DNA synthesis.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
• DOE Contract Number: W-7405-ENG-48
• Assignee: United States of America as represented by United States (Washington, DC)
• Patent Number(s): US 4585736
• OSTI ID: 865829
• Country of Publication: United States
• Language: English

Related Subjects

flow
cytometric
measurement
total
dna
incorporated
halodeoxyuridine
method
simultaneous
content
level
synthesis
normal
malignant
cells
disclosed
sensitivity
allows
study
cell
cycle
traverse
rates
scale
populations
single
measurements
stain
propidium
iodide
probe
monoclonal
antibody
reactive
precursor
bromodeoxyuridine
brdu
uptake
measure
monoclonal antibody
total dna
dna synthesis
dna content
flow cytometric
single cell
cell cycle
method allows
propidium iodide
malignant cells
cytometric measurement
cell populations
scale cell
simultaneous flow
incorporated halodeoxyuridine
method allow
/435/436/