Mutation assays involving blood cells that metabolize toxic substances

Patent · 01 January 1985

OSTI ID:865535

Crespi, Charles L ^[1]; Thilly, William G ^[2]

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1. Downers Grove, IL
2. Winchester, MA

A line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity) is disclosed. Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. Mutation assays using these cells, and other cells with similar characteristics, are also disclosed.

• Research Organization: Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA
• Other Award/Contract Number: AC04-76
• Assignee: Massachusetts Institute of Technology (Cambridge, MA)
• Patent Number(s): US 4532204
• OSTI ID: 865535
• Country of Publication: United States
• Language: English

Related Subjects

/435/
activity
assays
assays involving
blood
blood cell
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cells
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characteristics
culture
determine
disclosed
grow
human
human blood
hydroxylase
hydroxylase activity
involving
involving blood
levels
line
metabolize
metabolize toxic
metabolized
mutagenic
mutagenicity
mutation
mutation assays
oxidase
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oxygenase
peroxidase
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suspension culture
toxic
toxic substances
useful
xenobiotic