Feasibility studies of using the Catfish Immune System to produce monoclonal antibodies
The objective of these studies was to determine the feasibility of using a teleost cell line to produce monoclonal antibodies. Studies were undertaken to demonstrate the production of a polyclonal response of channel catfish (Icatalurus punctatus) challenged with mycotoxins coupled to a protein carrier. Companion studies were also performed to induce a permanent cell line with catfish lymphocytes. Attempts to demonstrate a polyclonal response to haptenized mycotoxins were inconclusive. Tests to induce an immortal, permanent cell line with benzene and x-ray irradiated cells were also inconclusive. 3 refs., 13 tabs.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (USA)
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 6714970
- Report Number(s):
- PNL-6185; ON: DE87007949
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
ANTIGENS
AQUATIC ORGANISMS
AROMATICS
BENZENE
CARCINOGENESIS
ENDOTOXINS
ENZYME IMMUNOASSAY
FEASIBILITY STUDIES
FISHES
FUNGI
HYDROCARBONS
IMMUNOASSAY
MATERIALS
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PATHOGENESIS
PLANTS
TOXIC MATERIALS
TOXINS
VERTEBRATES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ANTIBODIES
ANTIGENS
AQUATIC ORGANISMS
AROMATICS
BENZENE
CARCINOGENESIS
ENDOTOXINS
ENZYME IMMUNOASSAY
FEASIBILITY STUDIES
FISHES
FUNGI
HYDROCARBONS
IMMUNOASSAY
MATERIALS
MONOCLONAL ANTIBODIES
ORGANIC COMPOUNDS
PATHOGENESIS
PLANTS
TOXIC MATERIALS
TOXINS
VERTEBRATES