Preparation and characterization of monoclonal antibodies to swine lymphocyte antigens
Conference
·
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6991642
A panel of hybridoma lines were produced by the fusion of Sp2/0 myeloma cells and spleen cells from mice immunized with pig peripheral blood lymphocytes. Thirty-three stable hybridomas were produced which secreted monoclonal antibodies that reacted with pig lymphocytes, as determined by an ELISA screening procedure. These monoclonal antibodies were characterized by a complement-mediated cytotoxicity test and by flow cytometric analysis. The molecular weights of the antigens recognized by the monoclonal antibodies were determined by immunoprecipitation of /sup 125/I surface labeled lymphocytes, followed by SDS-PAGE. One monoclonal antibody, 7-34-1 (IgG2a), which reacted to all peripheral blood lymphocytes, precipitated a MHC class I molecule composed of a 50 kd heavy chain and a 12 kd light chain (..beta../sub 2/ microglobulin). This monoclonal antibody may prove to be an important reagent for the detection of SLA antigens on pig cells.
- Research Organization:
- Iowa State Univ., Ames
- OSTI ID:
- 6991642
- Report Number(s):
- CONF-8604222-
- Conference Information:
- Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CHEMICAL COMPOSITION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
HYBRIDOMAS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN CELLS
SWINE
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGENS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL FLOW SYSTEMS
CHEMICAL COMPOSITION
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
ELECTROPHORESIS
HYBRIDOMAS
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MICE
MOLECULAR STRUCTURE
MONOCLONAL ANTIBODIES
NUCLEI
ODD-EVEN NUCLEI
RADIOISOTOPES
RODENTS
SOMATIC CELLS
SPLEEN CELLS
SWINE
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES