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Monoclonal antibodies to antigens on human neutrophils, activated T lymphocytes, and acute leukemia blast cells

Journal Article · · Bull. Exp. Biol. Med. (Engl. Transl.); (United States)
DOI:https://doi.org/10.1007/BF00842345· OSTI ID:6905253
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The authors describe the production of two mouse hybridomas secreting monoclonal antibodies to antigenic determinants of the surface membranes of human neutrophils, activated T lymphocytes, and acute leukemic blast cells. The degree of lymphocyte stimulation was estimated from incorporation of /sup 3/H-thymidine with parallel microculture. Monoclonal antibodies of supernatants of hybridoma cultures shown here reacted in both immunofluorescence test and cytotoxicity test with surface membrane antigens on the majority of neutrophils and PHA-activated peripheral blood lymphocytes from healthy subjects, but did not give positive reactions with unactivated lymphocytes, adherent monocytes, erythrocytes, and alloantigen-stimulated lymphocytes.
Research Organization:
Central Research Institute of Hematology and Blood Transfusion, Moscow (USSR)
OSTI ID:
6905253
Journal Information:
Bull. Exp. Biol. Med. (Engl. Transl.); (United States), Journal Name: Bull. Exp. Biol. Med. (Engl. Transl.); (United States) Vol. 103:6; ISSN BEXBA
Country of Publication:
United States
Language:
English

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Related Subjects

550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
AZINES
BIOCHEMISTRY
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD FORMATION
BODY FLUIDS
CELL CONSTITUENTS
CELL CULTURES
CELL MEMBRANES
CHEMISTRY
CONNECTIVE TISSUE CELLS
CYTOCHEMISTRY
DISEASES
ERYTHROCYTES
HEMIC DISEASES
HETEROCYCLIC COMPOUNDS
HYBRIDOMAS
IMMUNE REACTIONS
IMMUNE SYSTEM DISEASES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
LEUKEMIA
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MAN
MATERIALS
MEMBRANES
MICE
MONOCLONAL ANTIBODIES
NEOPLASMS
NEUTROPHILS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PRIMATES
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RIBOSIDES
RODENTS
SOMATIC CELLS
THYMIDINE
TRACER TECHNIQUES
TUMOR CELLS
VERTEBRATES