Onco-fetal specific monoclonal antibodies, methods of preparation and use
Patent
·
OSTI ID:6081418
A process is described of preparing a hybridoma secreting oncofetal specific monoclonal antibodies, which comprises: (a) immunizing an animal with immunizing amounts of a non-proliferating syngeneic mid-gestation fetal cell preparation; (b) isolating immunized lymphocytes from the animal; and (c) fusing the lymphocytes under appropriate fusion conditions with an immortalizing cell line to thereby obtain the hybridoma. A hybridoma cell line produces a monoclonal antibody having the following specificity characteristics: (a) immune reactivity towards rodent mid-gestation antigens; (b) immune reactivity towards human onco-fetal tumor antigens; (c) substantially no immune reactivity towards rodent late gestational fetal tissue; and (d) substantially no immune reactivity towards human normal tissue.
- Assignee:
- South Alabama Medical Science Foundation, Mobile, AL
- Patent Number(s):
- US 4686180
- OSTI ID:
- 6081418
- Country of Publication:
- United States
- Language:
- English
Similar Records
Monoclonal antibodies: new agents for cancer detection and targeted therapy
In vivo tumor localization using tumor-specific monkey xenoantibody, alloantibody, and murine monoclonal xenoantibody. [Mice]
Preparation and characterization of monoclonal antibodies to swine lymphocyte antigens
Journal Article
·
Mon Dec 31 23:00:00 EST 1990
· Cancer Detection and Prevention; (United States)
·
OSTI ID:5397465
In vivo tumor localization using tumor-specific monkey xenoantibody, alloantibody, and murine monoclonal xenoantibody. [Mice]
Journal Article
·
Wed Dec 31 23:00:00 EST 1980
· Ann. Surg.; (United States)
·
OSTI ID:5638539
Preparation and characterization of monoclonal antibodies to swine lymphocyte antigens
Conference
·
Tue Mar 04 23:00:00 EST 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:6991642
Related Subjects
550200* -- Biochemistry
550600 -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DESIGN
FETUSES
HUMAN POPULATIONS
HYBRIDOMAS
IMMUNE REACTIONS
IMMUNITY
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MONOCLONAL ANTIBODIES
POPULATIONS
PRODUCTION
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES
550600 -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
ANTIBODIES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DESIGN
FETUSES
HUMAN POPULATIONS
HYBRIDOMAS
IMMUNE REACTIONS
IMMUNITY
LEUKOCYTES
LYMPHOCYTES
MAMMALS
MATERIALS
MONOCLONAL ANTIBODIES
POPULATIONS
PRODUCTION
RODENTS
SOMATIC CELLS
TISSUES
VERTEBRATES