Feasibility studies of using the Catfish Immune System to produce monoclonal antibodies

Technical Report · Sun Mar 01 00:00:00 EST 1987

DOI:https://doi.org/10.2172/6714970· OSTI ID:6714970

Poston, T M

The objective of these studies was to determine the feasibility of using a teleost cell line to produce monoclonal antibodies. Studies were undertaken to demonstrate the production of a polyclonal response of channel catfish (Icatalurus punctatus) challenged with mycotoxins coupled to a protein carrier. Companion studies were also performed to induce a permanent cell line with catfish lymphocytes. Attempts to demonstrate a polyclonal response to haptenized mycotoxins were inconclusive. Tests to induce an immortal, permanent cell line with benzene and x-ray irradiated cells were also inconclusive. 3 refs., 13 tabs.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• DOE Contract Number: AC06-76RL01830
• OSTI ID: 6714970
• Report Number(s): PNL-6185; ON: DE87007949
• Resource Relation: Other Information: Portions of this document are illegible in microfiche products. Original copy available until stock is exhausted
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BENZENE
CARCINOGENESIS
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MONOCLONAL ANTIBODIES
ENDOTOXINS
ENZYME IMMUNOASSAY
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