Mice are actively immunized after passive monoclonal antibody prophylaxis and ricin toxin challenge. (Reannouncement with new availability information)
Technical Report
·
OSTI ID:189794
Mice passively immunized by a protective, anti-ricin A-chain monoclonal antibody, then challenged intravenously with ricin, were protected from a subsequent ricin challenge, and were actively immunized. Two significant advantages accrued from this experiment: the monoclonal antibody neutralized the toxicity of the ricin immunogen, and active immunization was achieved with very low antigen load (approx. 0.5 micrograms/mouse). We ruled out the possibility that residual monoclonal antibody provided the protection by using three independent criteria. There was significant (four orders of magnitude) enhancement of the immune response in the presence of the monoclonal antibody; control immunizations of mice with ricin A-chain, ricin B-chain or either chain with the monoclonal antibody did not induce active immunity; and the active immunization could not be replicated when protective goat polyclonal antibody was substituted for the monoclonal antibody. Because high titers were achieved rapidly without any adjuvant, we are currently investigating haptenized ricin to determine if anti-hapten monoclonal antibodies can be produced by this refined procedure.
- Research Organization:
- Army Medical Research Inst. of Infectious Diseases, Fort Detrick, MD (United States)
- OSTI ID:
- 189794
- Report Number(s):
- AD-A--259562/7/XAB
- Country of Publication:
- United States
- Language:
- English
Similar Records
Development of monoclonal antibodies against parathyroid hormone: genetic control of the immune response to human PTH
Bone marrow transplantation across major histocompatibility barriers. V. Protection of mice from lethal graft-vs. -host disease by pretreatment of donor cells with monoclonal anti-Thy-1. 2 coupled to the toxin ricin
Prophylaxis and therapy of influenza pneumonia in mice by intratracheal instillation of monoclonal antibody
Journal Article
·
Mon Dec 31 23:00:00 EST 1984
· Methods Enzymol.; (United States)
·
OSTI ID:7092566
Bone marrow transplantation across major histocompatibility barriers. V. Protection of mice from lethal graft-vs. -host disease by pretreatment of donor cells with monoclonal anti-Thy-1. 2 coupled to the toxin ricin
Journal Article
·
Sun Feb 28 23:00:00 EST 1982
· J. Exp. Med.; (United States)
·
OSTI ID:5441329
Prophylaxis and therapy of influenza pneumonia in mice by intratracheal instillation of monoclonal antibody
Thesis/Dissertation
·
Mon Dec 31 23:00:00 EST 1984
·
OSTI ID:6655365