Development of monoclonal antibodies against parathyroid hormone: genetic control of the immune response to human PTH

Journal Article · Tue Jan 01 04:00:00 EST 1985 · Methods Enzymol.; (United States)

DOI:https://doi.org/10.1016/0076-6879(85)09119-4· OSTI ID:7092566

Nussbaum, S R; Lin, C S; Potts, Jr, J T; Rosenthal, A S; Rosenblatt, M

Seventeen monocloanl antibodies against the aminoterminal portion of parathyroid hormone (PTH) were generated by using BALB/c mouse for immunization fully biologically active synthetic human PTH-(1-34) and bovine PTH-(1-84) as immunogens, monoclonal antibody methods, and a solid-phase screening assay. Isotypic analysis of these monoclonal antibodies was performed using affinity purified goat antimouse immunoglobulins specific for IgG heavy chains and ..mu..(IgM). All antibodies were IgM as evidenced by 40 times greater than background activity when 25,000 cpm of /sup 125/I-labelled goat anti-mouse IgM was used as second antibody in a radioimmunoassay.

• OSTI ID: 7092566
• Journal Information: Methods Enzymol.; (United States), Journal Name: Methods Enzymol.; (United States) Vol. 109; ISSN MENZA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
CATTLE
DATA
DAYS LIVING RADIOISOTOPES
DOMESTIC ANIMALS
ELECTRON CAPTURE RADIOISOTOPES
EXPERIMENTAL DATA
GLOBULINS
GOATS
HORMONES
IMMUNE REACTIONS
IMMUNOASSAY
IMMUNOGLOBULINS
IMMUNOLOGY
INFORMATION
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MICE
MONOCLONAL ANTIBODIES
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARATHORMONE
PEPTIDE HORMONES
PROTEINS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RADIOISOTOPES
RODENTS
RUMINANTS
TRACER TECHNIQUES
VERTEBRATES