skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Phenolic acid esterases, coding sequences and methods

Patent ·
OSTI ID:874323

Described herein are four phenolic acid esterases, three of which correspond to domains of previously unknown function within bacterial xylanases, from XynY and XynZ of Clostridium thermocellum and from a xylanase of Ruminococcus. The fourth specifically exemplified xylanase is a protein encoded within the genome of Orpinomyces PC-2. The amino acids of these polypeptides and nucleotide sequences encoding them are provided. Recombinant host cells, expression vectors and methods for the recombinant production of phenolic acid esterases are also provided.

Research Organization:
Univ. of Georgia, Athens, GA (United States)
DOE Contract Number:
FG05-93ER20127
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA)
Patent Number(s):
US 6365390
OSTI ID:
874323
Country of Publication:
United States
Language:
English

References (20)

A cyclophilin from the polycentric anaerobic rumen fungus Orpinomyces sp. strain PC-2 is highly homologous to vertebrate cyclophilin B. journal March 1995
Plant cell wall degrading enzyme complexes from the cellulolytic rumen bacterium Ruminococcus flavefaciens journal May 1998
Expression of a Butyrivibrio fibrisolvens E14 gene (cinB) encoding an enzyme with cinnamoyl ester hydrolase activity is negatively regulated by the product of an adjacent gene (cinR) journal April 1997
Evidence for a general role for non-catalytic thermostabilizing domains in xylanases from thermophilic bacteria journal April 1995
Assay for trans-p-coumaroyl esterase using a specific substrate from plant cell walls journal October 1990
Esterases of xylan-degrading microorganisms: Production, properties, and significance journal June 1993
A modular esterase from Pseudomonas fluorescens subsp. cellulosa contains a non-catalytic cellulose-binding domain journal September 1993
Nucleotide Sequence of the Clostridium stercorarium xynA Gene Encoding Xylanase A: Identification of Catalytic and Cellulose Binding Domains journal January 1993
Cloning of a gene encoding cinnamoyl ester hydrolase from the ruminai bacterium Butyrivibrio fibrisolvens E14 by a novel method journal October 1996
Nucleotide sequence and deletion analysis of the xylanase gene (xynZ) of Clostridium thermocellum. journal October 1988
A bifunctional enzyme, with separate xylanase and beta(1,3-1,4)-glucanase domains, encoded by the xynD gene of Ruminococcus flavefaciens. journal January 1993
Esterase Activities of Fibrobacter succinogenes subsp. succinogenes S85 journal January 1990
Purification and characterization of a feruloyl/ρ-coumaroyl esterase from solid-state cultures of the aerobic fungus Penicillium pinophilum journal August 1992
The Cellulosome: The Exocellular Organelle of Clostridium journal October 1993
Purification and partial characterization of two feruloyl esterases from the anaerobic fungus Neocallimastix strain MC-2. journal January 1992
Ferulic Acid Esterase Activity from Schizophyllum commune† journal January 1988
Identification and Characterization of Cellulose-Binding Domains in Xylanase A of Clostridium stercorarium journal May 1996
The purification and characterization of 4-hydroxy-3-methoxycinnamic (ferulic) acid esterase from Streptomyces olivochromogenes journal October 1991
Lignin-ferulate cross-links in grasses: active incorporation of ferulate polysaccharide esters into ryegrass lignins journal September 1995
Butyrivibriospp. and Other Xylanolytic Microorganisms From the Rumen have Cinnamoyl Esterase Activity journal February 1998