Orpinomyces cellulase celf protein and coding sequences

Li, Xin-Liang; Chen, Huizhong; Ljungdahl, Lars G

Orpinomyces cellulase celf protein and coding sequences

Patent · Fri Dec 31 23:00:00 EST 1999

OSTI ID:873216

Li, Xin-Liang ^[1]; Chen, Huizhong ^[1]; Ljungdahl, Lars G ^[1]

Athens, GA

A cDNA (1,520 bp), designated celF, consisting of an open reading frame (ORF) encoding a polypeptide (CelF) of 432 amino acids was isolated from a cDNA library of the anaerobic rumen fungus Orpinomyces PC-2 constructed in Escherichia coli. Analysis of the deduced amino acid sequence showed that starting from the N-terminus, CelF consists of a signal peptide, a cellulose binding domain (CBD) followed by an extremely Asn-rich linker region which separate the CBD and the catalytic domains. The latter is located at the C-terminus. The catalytic domain of CelF is highly homologous to CelA and CelC of Orpinomyces PC-2, to CelA of Neocallimastix patriciarum and also to cellobiohydrolase IIs (CBHIIs) from aerobic fungi. However, Like CelA of Neocallimastix patriciarum, CelF does not have the noncatalytic repeated peptide domain (NCRPD) found in CelA and CelC from the same organism. The recombinant protein CelF hydrolyzes cellooligosaccharides in the pattern of CBHII, yielding only cellobiose as product with cellotetraose as the substrate. The genomic celF is interrupted by a 111 bp intron, located within the region coding for the CBD. The intron of the celF has features in common with genes from aerobic filamentous fungi.

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Research Organization:: University of Georgia

DOE Contract Number:: FG05-93ER20127

Assignee:: University of Georgia Research Foundation, Inc. (Athens, GA)

Patent Number(s):: US 6114158

Application Number:: 09/118319

OSTI ID:: 873216

Country of Publication:: United States

Language:: English

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Orpinomyces cellulase celf protein and coding sequences

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