Orpinomyces cellulase celf protein and coding sequences

Li, Xin-Liang; Chen, Huizhong; Ljungdahl, Lars G

Orpinomyces cellulase celf protein and coding sequences

Patent · Sat Jan 01 04:00:00 EST 2000

OSTI ID:873216

Li, Xin-Liang ^[1]; Chen, Huizhong ^[1]; Ljungdahl, Lars G ^[1]

Athens, GA

A cDNA (1,520 bp), designated celF, consisting of an open reading frame (ORF) encoding a polypeptide (CelF) of 432 amino acids was isolated from a cDNA library of the anaerobic rumen fungus Orpinomyces PC-2 constructed in Escherichia coli. Analysis of the deduced amino acid sequence showed that starting from the N-terminus, CelF consists of a signal peptide, a cellulose binding domain (CBD) followed by an extremely Asn-rich linker region which separate the CBD and the catalytic domains. The latter is located at the C-terminus. The catalytic domain of CelF is highly homologous to CelA and CelC of Orpinomyces PC-2, to CelA of Neocallimastix patriciarum and also to cellobiohydrolase IIs (CBHIIs) from aerobic fungi. However, Like CelA of Neocallimastix patriciarum, CelF does not have the noncatalytic repeated peptide domain (NCRPD) found in CelA and CelC from the same organism. The recombinant protein CelF hydrolyzes cellooligosaccharides in the pattern of CBHII, yielding only cellobiose as product with cellotetraose as the substrate. The genomic celF is interrupted by a 111 bp intron, located within the region coding for the CBD. The intron of the celF has features in common with genes from aerobic filamentous fungi.

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Research Organization:: University of Georgia

DOE Contract Number:: FG05-93ER20127

Assignee:: University of Georgia Research Foundation, Inc. (Athens, GA)

Patent Number(s):: US 6114158

Application Number:: 09/118319

OSTI ID:: 873216

Country of Publication:: United States

Language:: English

References (30)

Cello-Oligosaccharide Hydrolysis by Cellobiohydrolase II from Trichoderma Reesei. Association and Rate Constants Derived from an Analysis of Progress Curves Harjunpaa, Vesa; Teleman, Anita; Koivula, Anu European Journal of Biochemistry, Vol. 240, Issue 3 https://doi.org/10.1111/j.1432-1033.1996.0584h.x	journal	September 1996
Separation of endo- and exo-type cellulases using a new affinity chromatography method van Tilbeurgh, Herman; Bhikhabhai, Ramagauri; Göran Pettersson, L. FEBS Letters, Vol. 169, Issue 2, p. 215-218 https://doi.org/10.1016/0014-5793(84)80321-X	journal	April 1984
The Conserved Noncatalytic 40-Residue Sequence in Cellulases and Hemicellulases from Anaerobic Fungi Functions as a Protein Docking Domain Fanutti, Cristina; Ponyi, Tamás; Black, Gary W. Journal of Biological Chemistry, Vol. 270, Issue 49 https://doi.org/10.1074/jbc.270.49.29314	journal	December 1995
Homologous catalytic domains in a rumen fungal xylanase: evidence for gene duplication and prokaryotic origin Gilbert, H. J.; Hazlewood, G. P.; Laurie, J. I. Molecular Microbiology, Vol. 6, Issue 15 https://doi.org/10.1111/j.1365-2958.1992.tb01379.x	journal	August 1992
A cyclophilin from the polycentric anaerobic rumen fungus Orpinomyces sp. strain PC-2 is highly homologous to vertebrate cyclophilin B. Chen, H.; Li, X. L.; Ljungdahl, L. G. Proceedings of the National Academy of Sciences, Vol. 92, Issue 7 https://doi.org/10.1073/pnas.92.7.2587	journal	March 1995
Two genes of the anaerobic fungus Orpinomyces sp. strain PC-2 encoding cellulases with endoglucanase activities may have arisen by gene duplication Chen, Huizhong; Li, Xin-Liang; Blum, David L. FEMS Microbiology Letters, Vol. 159, Issue 1 https://doi.org/10.1111/j.1574-6968.1998.tb12842.x	journal	February 1998
Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea Filho, Edivaldo Ximenes Ferreira Canadian Journal of Microbiology, Vol. 42, Issue 1, p. 1-5 https://doi.org/10.1139/m96-001	journal	January 1996
Fermentation products and plant cell wall-degrading enzymes produced by monocentric and polycentric anaerobic ruminal fungi. Borneman, W. S.; Akin, D. E.; Ljungdahl, L. G. Applied and Environmental Microbiology, Vol. 55, Issue 5 https://doi.org/10.1128/AEM.55.5.1066-1073.1989	journal	January 1989
New families in the classification of glycosyl hydrolases based on amino acid sequence similarities Henrissat, B.; Bairoch, A. Biochemical Journal, Vol. 293, Issue 3, p. 781-788 https://doi.org/10.1042/bj2930781	journal	August 1993
Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families. Gilkes, N. R.; Henrissat, B.; Kilburn, D. G. Microbiological Reviews, Vol. 55, Issue 2 https://doi.org/10.1128/MR.55.2.303-315.1991	journal	January 1991
Sequencing of a 1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin. Chen, H.; Li, X. L.; Ljungdahl, L. G. Journal of bacteriology, Vol. 179, Issue 19 https://doi.org/10.1128/JB.179.19.6028-6034.1997	journal	January 1997
Homologous domains in Trichoderma reesei cellulolytic enzymes: Gene sequence and expression of cellobiohydrolase II Teeri, Tuula T.; Lehtovaara, Päivi; Kauppinen, Sakari Gene, Vol. 51, Issue 1, p. 43-52 https://doi.org/10.1016/0378-1119(87)90472-0	journal	January 1987
An endoglucanase from the anaerobic fungus Orpinomyces joyonii : characterization of the gene and its product Liu, Jin-Hao; Selinger, L. Brent; Hu, You-Ji Canadian Journal of Microbiology, Vol. 43, Issue 5 https://doi.org/10.1139/m97-067	journal	May 1997
The active site of Trichoderma reesei cellobiohydrolase II: the role of tyrosine 169 Koivula, Anu; Reinikainen, Tapani; Ruohonen, Laura "Protein Engineering, Design and Selection", Vol. 9, Issue 8 https://doi.org/10.1093/protein/9.8.691	journal	January 1996
Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase Zhou, L.; Xue, G. P.; Orpin, C. G. Biochemical Journal, Vol. 297, Issue 2 https://doi.org/10.1042/bj2970359	journal	January 1994
Adsorption characteristics of cellulolytic enzymes from the anaerobic fungus Piromyces sp. strain E2 on microcrystalline cellulose. Dijkerman, R.; Vervuren, M. B.; Op Den Camp, H. J. Applied and environmental microbiology, Vol. 62, Issue 1 https://doi.org/10.1128/AEM.62.1.20-25.1996	journal	January 1996
Xylanase B from Neocallimastix patriciarum contains a non-catalytic 455-residue linker sequence comprised of 57 repeats of an octapeptide Black, G. W.; Hazlewood, G. P.; Xue, G. P. Biochemical Journal, Vol. 299, Issue 2 https://doi.org/10.1042/bj2990381	journal	April 1994
Characterization of a Neocallimastix patriciarum cellulase cDNA (celA) homologous to Trichoderma reesei cellobiohydrolase II. Denman, S.; Xue, G. P.; Patel, B. Applied and environmental microbiology, Vol. 62, Issue 6 https://doi.org/10.1128/AEM.62.6.1889-1896.1996	journal	January 1996
Structural Role of Calcium for the Organization of the Cellulosome of Clostridium thermocellum ^† Choi, Sang Ki; Ljungdahl, Lars G. Biochemistry, Vol. 35, Issue 15 https://doi.org/10.1021/bi9524631	journal	January 1996
Molecular dynamics simulation of fungal cellulose-binding domains: differences in molecular rigidity but a preserved cellulose binding surface Hoffrén, Anna-Marja; Teeri, Tuula T.; Teleman, Olle "Protein Engineering, Design and Selection", Vol. 8, Issue 5 https://doi.org/10.1093/protein/8.5.443	journal	January 1995
The anaerobic fungus Neocallimastix frontalis: isolation and properties of a cellulosome-type enzyme fraction with the capacity to solubilize hydrogen-bond-ordered cellulose Wilson, CatrionaA.; Wood, ThomasM. Applied Microbiology and Biotechnology, Vol. 37, Issue 1 https://doi.org/10.1007/BF00174216	journal	April 1992
The use of conserved cellulase family-specific sequences to clone cellulase homologue cDNAs from Fusarium oxysporum Sheppard, Paul O.; Grant, Francis J.; Oort, Pieter J. Gene, Vol. 150, Issue 1 https://doi.org/10.1016/0378-1119(94)90878-8	journal	December 1994
Monocentric and polycentric anaerobic fungi produce structurally related cellulases and xylanases. Li, X. L.; Chen, H.; Ljungdahl, L. G. Applied and environmental microbiology, Vol. 63, Issue 2 https://doi.org/10.1128/AEM.63.2.628-635.1997	journal	January 1997
Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas Béguin, Pierre Analytical Biochemistry, Vol. 131, Issue 2 https://doi.org/10.1016/0003-2697(83)90178-1	journal	June 1983
Evidence that the Piromyces gene family encoding endo-l,4-mannanases arose through gene duplication Millward-Sadler, Sarah J.; Hall, Judith; Black, Gary W. FEMS Microbiology Letters, Vol. 141, Issue 2-3 https://doi.org/10.1111/j.1574-6968.1996.tb08382.x	journal	August 1996
Three Neocallimastix patriciarum esterases associated with the degradation of complex polysaccharides are members of a new family of hydrolases Dalrymple, B. P.; Cybinski, D. H.; Layton, I. Microbiology, Vol. 143, Issue 8 https://doi.org/10.1099/00221287-143-8-2605	journal	August 1997
Species-specificity of the cohesin-dockerin interaction betweenClostridium thermocellum andClostridium cellulolyticum: Prediction of specificity determinants of the dockerin domain Pagès, Sandrine; Bélaïch, Anne; Bélaïch, Jean-Pierre Proteins: Structure, Function, and Genetics, Vol. 29, Issue 4 https://doi.org/10.1002/(SICI)1097-0134(199712)29:4<517::AID-PROT11>3.0.CO;2-P	journal	December 1997
Two cellulases, CelA and CelC, from the polycentric anaerobic fungus Orpinomyces strain PC-2 contain N-terminal docking domains for a cellulase-hemicellulase complex. Li, X. L.; Chen, H.; Ljungdahl, L. G. Applied and environmental microbiology, Vol. 63, Issue 12 https://doi.org/10.1128/AEM.63.12.4721-4728.1997	journal	January 1997
Three-dimensional structure of cellobiohydrolase II from Trichoderma reesei Rouvinen, J.; Bergfors, T.; Teeri, T. Science, Vol. 249, Issue 4967 https://doi.org/10.1126/science.2377893	journal	July 1990
Molecular characterization of xyn3, a member of the endoxylanase multigene family of the rumen anaerobic fungus Neocallimastix frontalis Durand, R.; Rascle, Christine; Fèvre, Michel Current Genetics, Vol. 30, Issue 6 https://doi.org/10.1007/s002940050166	journal	December 1996

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Orpinomyces cellulase celf protein and coding sequences

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