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Orpinomyces cellulase CelE protein and coding sequences

Patent ·
OSTI ID:873198
 [1];  [1];  [1]
  1. Athens, GA
+ Show Author Affiliations
A CDNA designated celE cloned from Orpinomyces PC-2 encodes a polypeptide (CelE) of 477 amino acids. CelE is highly homologous to CelB of Orpinomyces (72.3% identity) and Neocallimastix (67.9% identity), and like them, it has a non-catalytic repeated peptide domain (NCRPD) at the C-terminal end. The catalytic domain of CelE is homologous to glycosyl hydrolases of Family 5, found in several anaerobic bacteria. The gene of celE is devoid of introns. The recombinant proteins CelE and CelB of Orpinomyces PC-2 randomly hydrolyze carboxymethylcellulose and cello-oligosaccharides in the pattern of endoglucanases.
Research Organization:
University of Georgia
DOE Contract Number:
FG05-93ER20127
Assignee:
University of Georgia Research Foundation, Inc. (Athens, GA)
Patent Number(s):
US 6110720
Application Number:
09/118324
OSTI ID:
873198
Country of Publication:
United States
Language:
English

References (21)

The Conserved Noncatalytic 40-Residue Sequence in Cellulases and Hemicellulases from Anaerobic Fungi Functions as a Protein Docking Domain journal December 1995
Homologous catalytic domains in a rumen fungal xylanase: evidence for gene duplication and prokaryotic origin journal August 1992
Two genes of the anaerobic fungus Orpinomyces sp. strain PC-2 encoding cellulases with endoglucanase activities may have arisen by gene duplication journal February 1998
Fermentation products and plant cell wall-degrading enzymes produced by monocentric and polycentric anaerobic ruminal fungi. journal January 1989
New families in the classification of glycosyl hydrolases based on amino acid sequence similarities journal August 1993
Sequencing of a 1,3-1,4-beta-D-glucanase (lichenase) from the anaerobic fungus Orpinomyces strain PC-2: properties of the enzyme expressed in Escherichia coli and evidence that the gene has a bacterial origin. journal January 1997
An endoglucanase from the anaerobic fungus Orpinomyces joyonii : characterization of the gene and its product journal May 1997
Intronless celB from the anaerobic fungus Neocallimastix patriciarum encodes a modular family A endoglucanase journal January 1994
Xylanase B from Neocallimastix patriciarum contains a non-catalytic 455-residue linker sequence comprised of 57 repeats of an octapeptide journal April 1994
Characterization of a Neocallimastix patriciarum cellulase cDNA (celA) homologous to Trichoderma reesei cellobiohydrolase II. journal January 1996
Structural Role of Calcium for the Organization of the Cellulosome of Clostridium thermocellum † journal January 1996
Cloning, sequencing, and regulation of a xylanase gene from the fungus Aureobasidium pullulans Y-2311-1. journal January 1994
Monocentric and polycentric anaerobic fungi produce structurally related cellulases and xylanases. journal January 1997
Detection of cellulase activity in polyacrylamide gels using Congo red-stained agar replicas journal June 1983
Evidence that the Piromyces gene family encoding endo-l,4-mannanases arose through gene duplication journal August 1996
Three Neocallimastix patriciarum esterases associated with the degradation of complex polysaccharides are members of a new family of hydrolases journal August 1997
Cellulase families and their genes journal September 1987
Molecular cloning of genes from the rumen anaerobic fungus Neocallimastix frontalis : expression during hydrolase induction journal January 1991
Two cellulases, CelA and CelC, from the polycentric anaerobic fungus Orpinomyces strain PC-2 contain N-terminal docking domains for a cellulase-hemicellulase complex. journal January 1997
Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in Escherichia coli journal July 1992
Molecular characterization of xyn3, a member of the endoxylanase multigene family of the rumen anaerobic fungus Neocallimastix frontalis journal December 1996

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