RNA-guided nucleic acid modifying enzymes and methods of use thereof

Doudna, Jennifer A.; Banfield, Jillian; Burstein, David; Harrington, Lucas Benjamin; Strutt, Steven C.

Title: RNA-guided nucleic acid modifying enzymes and methods of use thereof

Patent · Tue Oct 24 00:00:00 EDT 2023

OSTI ID:2293753

Doudna, Jennifer A.; Banfield, Jillian; Burstein, David; Harrington, Lucas Benjamin; Strutt, Steven C.

The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

View Patent

Cite

Export

Save

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE; National Science Foundation (NSF)

DOE Contract Number:: 1244557; AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 11,795,472

Application Number:: 16/422,824

OSTI ID:: 2293753

Resource Relation:: Patent File Date: 05/24/2019

Country of Publication:: United States

Language:: English

References (90)

An updated evolutionary classification of CRISPR–Cas systems Makarova, Kira S.; Wolf, Yuri I.; Alkhnbashi, Omer S. Nature Reviews Microbiology, Vol. 13, Issue 11, p. 722-736 https://doi.org/10.1038/nrmicro3569	journal	September 2015
SnapShot: Class 2 CRISPR-Cas Systems Makarova, Kira S.; Zhang, Feng; Koonin, Eugene V. Cell, Vol. 168, Issue 1-2 https://doi.org/10.1016/j.cell.2016.12.038	journal	January 2017
Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering Wright, Addison V.; Nuñez, James K.; Doudna, Jennifer A. Cell, Vol. 164, Issue 1-2 https://doi.org/10.1016/j.cell.2015.12.035	journal	January 2016
CRISPR enzymes and systems Zhang, Feng; Zetsche, Bernd; Slaymaker, Ian https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9790490 US Patent Document 9,790,490	patent	October 2017
Origins and evolution of CRISPR-Cas systems Koonin, Eugene V.; Makarova, Kira S. Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 374, Issue 1772 https://doi.org/10.1098/rstb.2018.0087	journal	March 2019
RNA-Guided Nucleic Acid Modifying Enzymes and Methods of Use Thereof Doudna, Jennifer A.; Banfield, Jillian F.; Burstein, David https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20190300908 US Patent Application 16/335630; 20190300908	patent-application	October 2019
CRISPR effector system based diagnostics Abudayyeh, Omar; Collins, James J.; Gootenberg, Jonathan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10266886 US Patent Document 10,266,886	patent	April 2019
Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities Liu, Liang; Li, Xueyan; Wang, Jiuyu Cell, Vol. 168, Issue 1-2 https://doi.org/10.1016/j.cell.2016.12.031	journal	January 2017
Methods and compositions for detecting a target RNA Doudna, Jennifer A.; O'Connell, Mitchell; East-Seletsky, Alexandra https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10337051 US Patent Document 10,337,051	patent	July 2019
Compositions and methods for visual ribonuclease detection assays Walder, Joseph A.; Behlke, Mark Aaron; Devor, Eric J. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6773885 US Patent Document 6,773,885	patent	August 2004
A CRISPR/Cas system mediates bacterial innate immune evasion and virulence Sampson, Timothy R.; Saroj, Sunil D.; Llewellyn, Anna C. Nature, Vol. 497, Issue 7448 https://doi.org/10.1038/nature12048	journal	April 2013
Computational Complexity, Protein Structure Prediction, and the Levinthal Paradox Ngo, J. Thomas; Marks, Joe; Karplus, Martin The Protein Folding Problem and Tertiary Structure Prediction, p. 433-506 https://doi.org/10.1007/978-1-4684-6831-1_14	book	January 1994
C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector Abudayyeh, Omar O.; Gootenberg, Jonathan S.; Konermann, Silvana Science, Vol. 353, Issue 6299 https://doi.org/10.1126/science.aaf5573	journal	June 2016
RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes East-Seletsky, Alexandra; O’Connell, Mitchell R.; Burstein, David Molecular Cell, Vol. 66, Issue 3 https://doi.org/10.1016/j.molcel.2017.04.008	journal	May 2017
Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6 Gootenberg, Jonathan S.; Abudayyeh, Omar O.; Kellner, Max J. Science, Vol. 360, Issue 6387 https://doi.org/10.1126/science.aaq0179	journal	February 2018
Use of DNAzymes for analysis of an RNA sample Zeiner, Gusti; Ach, Robert A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8815782 US Patent Document 8,815,782	patent	August 2014
RNA-guided nucleic acid modifying enzymes and methods of use thereof Doudna, Jennifer A.; Banfield, Jillian F.; Burstein, David https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10570415 US Patent Document 10,570,415	patent	February 2020
Using molecular beacons as a sensitive fluorescence assay for enzymatic cleavage of single-stranded DNA Li, J. J. Nucleic Acids Research, Vol. 28, Issue 11 https://doi.org/10.1093/nar/28.11.e52	journal	June 2000
Nucleic acid detection with CRISPR-Cas13a/C2c2 Gootenberg, Jonathan S.; Abudayyeh, Omar O.; Lee, Jeong Wook Science, Vol. 356, Issue 6336 https://doi.org/10.1126/science.aam9321	journal	April 2017
Diversity and evolution of class 2 CRISPR–Cas systems Shmakov, Sergey; Smargon, Aaron; Scott, David Nature Reviews Microbiology, Vol. 15, Issue 3 https://doi.org/10.1038/nrmicro.2016.184	journal	January 2017
Methods And Compositions For Rna-Directed Target Dna Modification And For Rna-Directed Modulation Of Transcription Doudna, Jennifer A.; Jinek, Martin; Charpentier, Emmanuelle https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20140068797 US Patent Application 13/842859; 20140068797	patent-application	March 2014
Compositions and Methods for Modifying Genomes Begemann, Matthew; Gray, Benjamin Neil https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170233756 US Patent Application 15/432109; 20170233756	patent-application	August 2017
Nicking and Extension Amplification Reaction for the Exponential Amplification of Nucleic Acids Maples, Brian K.; Holmberg, Rebecca C.; Miller, Andrew P. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20140093883 US Patent Application 14/067620; 20140093883	patent-application	April 2014
RNA-Guided Nucleic Acid Modifying Enzymes and Methods of Use Thereof Doudna, Jennifer A.; Banfield, Jilian; Burstein, David https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20190276842 US Patent Application 16/422824; 20190276842	patent-application	September 2019
Compositions and methods for modifying genomes Begemann, Matthew; Gray, Benjamin Neil https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10316324 US Patent Document 10,316,324	patent	June 2019
Expanding the CRISPR Toolbox: Targeting RNA with Cas13b Barrangou, Rodolphe; Gersbach, Charles A. Molecular Cell, Vol. 65, Issue 4 https://doi.org/10.1016/j.molcel.2017.02.002	journal	February 2017
Methods and Compositions for Using Argonaute to Modify a Single Stranded Target Nucleic Acid Doudna, Jennifer A.; Kaya, Emine; Knoll, Kilian R. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170175104 US Patent Application 15/129382; 20170175104	patent-application	June 2017
CasX enzymes comprise a distinct family of RNA-guided genome editors Liu, Jun-Jie; Orlova, Natalia; Oakes, Benjamin L. Nature, Vol. 566, Issue 7743 https://doi.org/10.1038/s41586-019-0908-x	journal	February 2019
Highly Sensitive Nuclease Assays Based on Chemically Modified DNA or RNA Sato, Shinobu; Takenaka, Shigeori Sensors, Vol. 14, Issue 7 https://doi.org/10.3390/s140712437	journal	July 2014
CRISPR-Cas: an adaptive immunity system in prokaryotes Koonin, Eugene V.; Makarova, Kira S. F1000 Biology Reports, Vol. 1 https://doi.org/10.3410/B1-95	journal	December 2009
RNA editing with CRISPR-Cas13 Cox, David B. T.; Gootenberg, Jonathan S.; Abudayyeh, Omar O. Science, Vol. 358, Issue 6366 https://doi.org/10.1126/science.aaq0180	journal	October 2017
The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a Liu, Liang; Li, Xueyan; Ma, Jun Cell, Vol. 170, Issue 4 https://doi.org/10.1016/j.cell.2017.06.050	journal	August 2017
Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein Yan, Winston X.; Chong, Shaorong; Zhang, Huaibin Molecular Cell, Vol. 70, Issue 2 https://doi.org/10.1016/j.molcel.2018.02.028	journal	April 2018
Classification and evolution of type II CRISPR-Cas systems Chylinski, Krzysztof; Makarova, Kira S.; Charpentier, Emmanuelle Nucleic Acids Research, Vol. 42, Issue 10 https://doi.org/10.1093/nar/gku241	journal	April 2014
The Bacteriophage Carrier State of Campylobacter jejuni Features Changes in Host Non-coding RNAs and the Acquisition of New Host-derived CRISPR Spacer Sequences Hooton, Steven P. T.; Brathwaite, Kelly J.; Connerton, Ian F. Frontiers in Microbiology, Vol. 7 https://doi.org/10.3389/fmicb.2016.00355	journal	March 2016
Methods and Compositions for Modifying a Single Stranded Target Nucleic Acid Doudna, Jennifer A.; Sternberg, Samuel H.; O'Connell, Mitchell https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160289659 Us Patent Application 15/036298; 20160289659 A1	patent-application	October 2016
Diversity, classification and evolution of CRISPR-Cas systems Koonin, Eugene V.; Makarova, Kira S.; Zhang, Feng Current Opinion in Microbiology, Vol. 37 https://doi.org/10.1016/j.mib.2017.05.008	journal	June 2017
Hairpin-Forming Peptide Nucleic Acid Oligomers Armitage, Bruce; Ly, Danith; Koch, Troels Biochemistry, Vol. 37, Issue 26 https://doi.org/10.1021/bi9729458	journal	June 1998
CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity Chen, Janice S.; Ma, Enbo; Harrington, Lucas B. Science, Vol. 360, Issue 6387 https://doi.org/10.1126/science.aar6245	journal	February 2018
Delivery strategies of the CRISPR-Cas9 gene-editing system for therapeutic applications Liu, Chang; Zhang, Li; Liu, Hao Journal of Controlled Release, Vol. 266 https://doi.org/10.1016/j.jconrel.2017.09.012	journal	November 2017
Phylogeny of Cas9 determines functional exchangeability of dual-RNA and Cas9 among orthologous type II CRISPR-Cas systems Fonfara, Ines; Le Rhun, Anaïs; Chylinski, Krzysztof Nucleic Acids Research, Vol. 42, Issue 4 https://doi.org/10.1093/nar/gkt1074	journal	November 2013
Virus-Induced Dormancy in the Archaeon Sulfolobus islandicus Bautista, Maria A.; Zhang, Changyi; Whitaker, Rachel J. mBio, Vol. 6, Issue 2 https://doi.org/10.1128/mBio.02565-14	journal	May 2015
Novel CRISPR Enzymes and Systems Smargon, Aaron Andrew; Zhang, Fenq https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170211142 US Patent Application 15/331792; 20170211142	patent-application	July 2017
New CRISPR-Cas systems discovered Yang, Hui; Patel, Dinshaw J. Cell Research, Vol. 27, Issue 3 https://doi.org/10.1038/cr.2017.21	journal	February 2017
Multiplex Genome Engineering Using CRISPR/Cas Systems Cong, L.; Ran, F. A.; Cox, D. Science, Vol. 339, Issue 6121, p. 819-823 https://doi.org/10.1126/science.1231143	journal	January 2013
RNA-Targeting System Zhang, Feng; Hsu, Patrick; Gootenberg, Jonathan S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170306335 US Patent Application 15/632067; 20170306335	patent-application	October 2017
Cas9-mediated targeting of viral RNA in eukaryotic cells Price, Aryn A.; Sampson, Timothy R.; Ratner, Hannah K. Proceedings of the National Academy of Sciences, Vol. 112, Issue 19 https://doi.org/10.1073/pnas.1422340112	journal	April 2015
Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA Yamano, Takashi; Nishimasu, Hiroshi; Zetsche, Bernd Cell, Vol. 165, Issue 4 https://doi.org/10.1016/j.cell.2016.04.003	journal	May 2016
Novel CRISPR Enzymes and Systems Severinov, Konstantin; Zhang, Feng; Wolf, Yuri I. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170321198 US Patent Application 15/482603; 20170321198	patent-application	November 2017
Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System Zetsche, Bernd; Gootenberg, Jonathan S.; Abudayyeh, Omar O. Cell, Vol. 163, Issue 3, p. 759-771 https://doi.org/10.1016/j.cell.2015.09.038	journal	October 2015
Method and system for treating cancer cachexia Kovarik, Katherine Rose; Kovarik, Joseph E. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9730967 US Patent Document 9,730,967	patent	August 2017
Compositions and Methods of Engineered CRISPR-CAS9 Systems Using Split-Nexus CAS9-Associated Polynucleotides Donohoue, Paul Daniel; May, Andrew Paul https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170037432 US Patent Application 14/835675; 20170037432	patent-application	February 2017
Programmed DNA destruction by miniature CRISPR-Cas14 enzymes Harrington, Lucas B.; Burstein, David; Chen, Janice S. Science, Vol. 362, Issue 6416 https://doi.org/10.1126/science.aav4294	journal	October 2018
Novel CRISPR Enzymes and Systems Zhang, Feng; Zetsche, Bernd; Slaymaker, Ian https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160208243 US Patent Application 14/975085; 20160208243	patent-application	July 2016
Enigmatic, ultrasmall, uncultivated Archaea Baker, B. J.; Comolli, L. R.; Dick, G. J. Proceedings of the National Academy of Sciences, Vol. 107, Issue 19 https://doi.org/10.1073/pnas.0914470107	journal	April 2010
RNA targeting with CRISPR–Cas13 Abudayyeh, Omar O.; Gootenberg, Jonathan S.; Essletzbichler, Patrick Nature, Vol. 550, Issue 7675 https://doi.org/10.1038/nature24049	journal	October 2017
CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III Deltcheva, Elitza; Chylinski, Krzysztof; Sharma, Cynthia M. Nature, Vol. 471, Issue 7340 https://doi.org/10.1038/nature09886	journal	March 2011
New CRISPR–Cas systems from uncultivated microbes Burstein, David; Harrington, Lucas B.; Strutt, Steven C. Nature, Vol. 542, Issue 7640 https://doi.org/10.1038/nature21059	journal	December 2016
Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles Hyun, Youbong; Kim, Jungeun; Cho, Seung Woo Planta, Vol. 241, Issue 1 https://doi.org/10.1007/s00425-014-2180-5	journal	October 2014
CRISPR/CAS Systems for Genomic Modification and Gene Modulation Wu, Fangting https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20140273226 US Patent Application 14/216655; 20140273226	patent-application	September 2014
Thousands of microbial genomes shed light on interconnected biogeochemical processes in an aquifer system Anantharaman, Karthik; Brown, Christopher T.; Hug, Laura A. Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms13219	journal	October 2016
Methods and Compositions for RNA-Directed Target DNA Modification and for RNA-Directed Modulation of Transcription Doudna, Jennifer A.; Jinel, Martin; Chylinski, Krzysztof https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20160138008 US Patent Application 14/942782; 20160138008	patent-application	May 2016
Target RNA capture and cleavage by the Cmr type III-B CRISPR–Cas effector complex Hale, Caryn R.; Cocozaki, Alexis; Li, Hong Genes & Development, Vol. 28, Issue 21 https://doi.org/10.1101/gad.250712.114	journal	November 2014
Methods for Identifying Class 2 CRISPR-CAS Systems Shmakov, Sergey; Makarova, Kira S.; Wolf, Yuri I. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20210166783 US Patent Application 16/326132; 20210166783	patent-application	June 2021
Guide-bound structures of an RNA-targeting A-cleaving CRISPR–Cas13a enzyme Knott, Gavin J.; East-Seletsky, Alexandra; Cofsky, Joshua C. Nature Structural & Molecular Biology, Vol. 24, Issue 10 https://doi.org/10.1038/nsmb.3466	journal	September 2017
Nucleic Acids For Multiplex Organism Detection and Methods Of Use And Making The Same Diamond, Lisa; Kumm, Jochen; Rolfe, Philip Alexander https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20130261196 US Patent Application 13/703489; 20130261196	patent-application	October 2013
Diverse evolutionary roots and mechanistic variations of the CRISPR-Cas systems Mohanraju, Prarthana; Makarova, Kira S.; Zetsche, Bernd Science, Vol. 353, Issue 6299 https://doi.org/10.1126/science.aad5147	journal	August 2016
RNA-Guided RNA Cleavage by a CRISPR RNA-Cas Protein Complex Hale, Caryn R.; Zhao, Peng; Olson, Sara Cell, Vol. 139, Issue 5 https://doi.org/10.1016/j.cell.2009.07.040	journal	November 2009
Methods and materials for detecting viral or microbial infections Smith, Kenneth D.; Yazvenko, Nina; Smit, Mariya https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/8597886 US Patent Document 8,597,886	patent	December 2013
CAS9-based Isothermal Method of Detection of Specific DNA Sequence Carstens, Carsten-Peter https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20150211058 US Patent Application 14/607781; 20150211058	patent-application	July 2015
CRISPR-dCas9 mediated TET1 targeting for selective DNA demethylation at BRCA1 promoter Choudhury, Samrat Roy; Cui, Yi; Lubecka, Katarzyna Oncotarget, Vol. 7, Issue 29 https://doi.org/10.18632/oncotarget.10234	journal	June 2016
Compositions and Methods for Improving Homogeneity of DNA Generated Using a CRISPR/Cas9 Cleavage System Kmiec, Eric Brian; Bialk, Pawel Alexander https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170198277 US Patent Application 15/402833; 20170198277	patent-application	July 2017
Hypersensitive substrate for ribonucleases Kelemen, B. R.; Klink, T. A.; Behike, M. A. Nucleic Acids Research, Vol. 27, Issue 18 https://doi.org/10.1093/nar/27.18.3696	journal	September 1999
CRISPR Effector System Based Diagnostics Abudayyeh, Omar; Collins, James Joseph; Gootenberg, Jonathan https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20180340218 US Patent Application 15/917546; 20180340218	patent-application	November 2018
Specific and sensitive detection of nucleic acids and RNases using gold nanoparticle–RNA–fluorescent dye conjugates Kim, Joong H.; Estabrook, R. August; Braun, Gary Chemical Communications, Issue 42 https://doi.org/10.1039/b710306a	journal	January 2007
Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants Makarova, Kira S.; Wolf, Yuri I.; Iranzo, Jaime Nature Reviews Microbiology, Vol. 18, Issue 2 https://doi.org/10.1038/s41579-019-0299-x	journal	December 2019
Class 2 CRISPR–Cas RNA-guided endonucleases: Swiss Army knives of genome editing Stella, Stefano; Alcón, Pablo; Montoya, Guillermo Nature Structural & Molecular Biology, Vol. 24, Issue 11 https://doi.org/10.1038/nsmb.3486	journal	October 2017
Methods and compositions for detecting a target RNA Doudna, Jennifer A.; O'Connell, Mitchell; East-Seletsky, Alexandra https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10494664 US Patent Document 10,494,664	patent	December 2019
Zinc Fingers, TAL Effectors, or Cas9-Based DNA Binding Proteins: What’s Best for Targeting Desired Genome Loci? Strauβ, Annett; Lahaye, Thomas Molecular Plant, Vol. 6, Issue 5 https://doi.org/10.1093/mp/sst075	journal	September 2013
Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems Shmakov, Sergey; Abudayyeh, Omar O.; Makarova, Kira S. Molecular Cell, Vol. 60, Issue 3, p. 385-397 https://doi.org/10.1016/j.molcel.2015.10.008	journal	November 2015
Compositions And Methods Of Nucleic Acid-Targeting Nucleic Acids May, Andrew Paul; Haurwitz, Rachel E.; Doudna, Jennifer A. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170051276 US Patent Application 15/344487; 20170051276	patent-application	February 2017
Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection East-Seletsky, Alexandra; O’Connell, Mitchell R.; Knight, Spencer C. Nature, Vol. 538, Issue 7624 https://doi.org/10.1038/nature19802	journal	September 2016
Cas13b Is a Type VI-B CRISPR-Associated RNA-Guided RNase Differentially Regulated by Accessory Proteins Csx27 and Csx28 Smargon, Aaron A.; Cox, David B. T.; Pyzocha, Neena K. Molecular Cell, Vol. 65, Issue 4 https://doi.org/10.1016/j.molcel.2016.12.023	journal	February 2017
Method for Generating Barcoded Combinatorial Libraries Gil, Ryan T.; Garst, Andrew; Lipscomb, Tanya Elizabeth https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170369870 Us Patent Application 15/632222; 20170369870	patent-application	December 2017
Molecular Mechanisms of RNA Targeting by Cas13-containing Type VI CRISPR–Cas Systems O'Connell, Mitchell R. Journal of Molecular Biology, Vol. 431, Issue 1 https://doi.org/10.1016/j.jmb.2018.06.029	journal	January 2019
Dead Guides for Crisper Transcription Factors Zhang, Feng; Konermann, Silvana; Dahlman, James https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20170321214 US Patent Application 15/620391; 20170321214	patent-application	November 2017
RNA-Guided Genome Editing in Plants Using a CRISPR–Cas System Xie, Kabin; Yang, Yinong Molecular Plant, Vol. 6, Issue 6 https://doi.org/10.1093/mp/sst119	journal	November 2013
Type V CRISPR/Cas effector proteins for cleaving ssDNAs and detecting target DNAs Doudna, Jennifer A.; Chen, Janice S.; Harrington, Lucas Benjamin https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/10253365 US Patent Document 10,253,365	patent	April 2019
RNA-Guided Nucleic Acid Modifying Enzymes and Methods of Use Thereof Doudna, Jennifer A.; Banfield, Jillian F.; Burstein, David https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20200017879 US Patent Application 16/335516; 20200017879	patent-application	January 2020
CASZ Compositions and Methods of Use Doudna, Jennifer A.; Burstein, David; Chen, Janice S. https://doi.org/https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20200087640 US Patent Application 16/694720; 20200087640	patent-application	March 2020

Similar Records

RNA-guided nucleic acid modifying enzymes and methods of use thereof

Patent · Tue Feb 25 00:00:00 EST 2020 · OSTI ID:2293753

Doudna, Jennifer A.; Banfield, Jillian; Burstein, David; +2 more

RNA-guided nucleic acid modifying enzymes and methods of use thereof

Patent · Tue Jun 28 00:00:00 EDT 2022 · OSTI ID:2293753

Doudna, Jennifer A.; Banfield, Jillian F.; Burstein, David; +1 more

CasZ compositions and methods of use

Patent · Tue Jun 28 00:00:00 EDT 2022 · OSTI ID:2293753

Doudna, Jennifer A.; Burstein, David; Chen, Janice Sha; +3 more

Title: RNA-guided nucleic acid modifying enzymes and methods of use thereof

Citation Formats

References (90)

Similar Records

Related Subjects