RNA-guided nucleic acid modifying enzymes and methods of use thereof

Doudna, Jennifer A.; Banfield, Jillian F.; Burstein, David; Harrington, Lucas Benjamin

RNA-guided nucleic acid modifying enzymes and methods of use thereof

Patent · Tue Jun 28 00:00:00 EDT 2022

OSTI ID:1893077

Doudna, Jennifer A.; Banfield, Jillian F.; Burstein, David; Harrington, Lucas Benjamin

The present disclosure provides CasY proteins, nucleic acids encoding the CasY proteins, and modified host cells comprising the CasY proteins and/or nucleic acids encoding same. CasY proteins are useful in a variety of applications, which are provided. The present disclosure provides CasY guide RNAs that bind to and provide sequence specificity to the CasY proteins, nucleic acids encoding the CasY guide RNAs, and modified host cells comprising the CasY guide RNAs and/or nucleic acids encoding same. CasY guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides methods of identifying a CRISPR RNA-guided endonuclease.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE; National Science Foundation (NSF)

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 11,371,062

Application Number:: 16/335,630

OSTI ID:: 1893077

Country of Publication:: United States

Language:: English

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