RNA-guided nucleic acid modifying enzymes and methods of use thereof

Doudna, Jennifer A.; Banfield, Jillian; Burstein, David; Harrington, Lucas Benjamin; Strutt, Steven C.

RNA-guided nucleic acid modifying enzymes and methods of use thereof

Patent · Tue Oct 24 04:00:00 EDT 2023

OSTI ID:2293753

Doudna, Jennifer A.; Banfield, Jillian; Burstein, David; Harrington, Lucas Benjamin; Strutt, Steven C.

The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE; National Science Foundation (NSF)

DOE Contract Number:: AC02-05CH11231

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Number(s):: 11,795,472

Application Number:: 16/422,824

OSTI ID:: 2293753

Country of Publication:: United States

Language:: English

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