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Title: RNA-guided nucleic acid modifying enzymes and methods of use thereof

Abstract

The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1632606
Patent Number(s):
10,570,415
Application Number:
16/054,513
Assignee:
The Regents of the University of California (Oakland, CA)
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 08/03/2018
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Doudna, Jennifer A., Banfield, Jillian, Burstein, David, Harrington, Lucas Benjamin, and Strutt, Steven C. RNA-guided nucleic acid modifying enzymes and methods of use thereof. United States: N. p., 2020. Web.
Doudna, Jennifer A., Banfield, Jillian, Burstein, David, Harrington, Lucas Benjamin, & Strutt, Steven C. RNA-guided nucleic acid modifying enzymes and methods of use thereof. United States.
Doudna, Jennifer A., Banfield, Jillian, Burstein, David, Harrington, Lucas Benjamin, and Strutt, Steven C. Tue . "RNA-guided nucleic acid modifying enzymes and methods of use thereof". United States. https://www.osti.gov/servlets/purl/1632606.
@article{osti_1632606,
title = {RNA-guided nucleic acid modifying enzymes and methods of use thereof},
author = {Doudna, Jennifer A. and Banfield, Jillian and Burstein, David and Harrington, Lucas Benjamin and Strutt, Steven C.},
abstractNote = {The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {2}
}

Patent:

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