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Title: XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves

Journal Article · · PeerJ
DOI:https://doi.org/10.7717/peerj.2446· OSTI ID:1904156
 [1];  [2];  [1];  [3];  [1];  [4];  [4];  [1]
  1. Univ. of California, Davis, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States)
  2. Univ. of California, Davis, CA (United States); Joint BioEnergy Institute (JBEI), Emeryville, CA (United States); Australian National Univ., Acton, ACT (Australia)
  3. Univ. of California, Davis, CA (United States); Chinese Academy of Agricultural Sciences, Beijing (China)
  4. Univ. of California, Davis, CA (United States)
+ Show Author Affiliations

The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzaepv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis, we identified eight genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.

Research Organization:
USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH)
Grant/Contract Number:
AC02-05CH11231; GM59962
OSTI ID:
1904156
Journal Information:
PeerJ, Vol. 4; ISSN 2167-8359
Publisher:
PeerJ Inc.Copyright Statement
Country of Publication:
United States
Language:
English

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Cited By (1)

The Rice Xa3 Gene Confers Resistance to Xanthomonas oryzae pv. oryzae in the Model Rice Kitaake Genetic Background journal February 2020

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
XA21
Xanthomonas
EFR
Rice
RNAseq