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Title: Mutation of the rice XA21 predicted nuclear localization sequence does not affect resistance to Xanthomonas oryzae pv. oryzae

Journal Article · · PeerJ
DOI:https://doi.org/10.7717/peerj.2507· OSTI ID:1378728
 [1];  [2];  [2];  [1]
  1. Univ. of California, Davis, CA (United States). Department of Plant Pathology and the Genome Center; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Feedstocks Division, Joint BioEnergy Institute and Biological Systems and Engineering Division
  2. Univ. of California, Davis, CA (United States). Department of Plant Pathology and the Genome Center
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Background: The rice receptor kinase XA21 confers robust resistance to the bacterial pathogenXanthomonas oryzae pv.oryzae(Xoo). We previously reported that XA21 is cleaved in transgenic plants overexpressing XA21 with a GFP tag (Ubi-XA21-GFP) and that the released C-terminal domain is localized to the nucleus. XA21 carries a predicted nuclear localization sequence (NLS) that directs the C-terminal domain to the nucleus in transient assays, whereas alanine substitutions in the NLS disrupt the nuclear localization. Methods: To determine if the predicted NLS is required for XA21-mediated immunityin planta, we generated transgenic plants overexpressing an XA21 variant carrying the NLS with the same alanine substitutions (Ubi-XA21nls-GFP). Results: Ubi- XA21nls-GFP plants displayed slightly longer lesion lengths, higherXoo bacterial populations after inoculation and lower levels of reactive oxygen species production compared with the Ubi- XA21-GFP control plants. However, the Ubi- XA21nls-GFP plants express lower levels of protein than that observed inUbi- XA21-GFP. Discussion: These results demonstrate that the predicted NLS is not required for XA21-mediated immunity.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1378728
Journal Information:
PeerJ, Vol. 4, Issue 10; ISSN 2167-8359
Publisher:
PeerJ Inc.Copyright Statement
Country of Publication:
United States
Language:
English
Citation Metrics:
Cited by: 4 works
Citation information provided by
Web of Science

References (14)

The Xanthomonas Ax21 protein is processed by the general secretory system and is secreted in association with outer membrane vesicles journal January 2014
Rice XB15, a Protein Phosphatase 2C, Negatively Regulates Cell Death and XA21-Mediated Innate Immunity journal September 2008
A Receptor Kinase-Like Protein Encoded by the Rice Disease Resistance Gene, Xa21 journal December 1995
Towards Establishment of a Rice Stress Response Interactome journal April 2011
Plant and Animal Sensors of Conserved Microbial Signatures journal November 2010
Overexpression of the Endoplasmic Reticulum Chaperone BiP3 Regulates XA21-Mediated Innate Immunity in Rice journal February 2010
Rice NRR, a negative regulator of disease resistance, interacts with Arabidopsis NPR1 and rice NH1: NRR, a negative regulator of resistance journal August 2005
Cleavage and nuclear localization of the rice XA21 immune receptor journal January 2012
The rice immune receptor XA21 recognizes a tyrosine-sulfated protein from a Gram-negative bacterium journal July 2015
Rice XA21 Binding Protein 3 Is a Ubiquitin Ligase Required for Full Xa21 -Mediated Disease Resistance journal December 2006
An ATPase promotes autophosphorylation of the pattern recognition receptor XA21 and inhibits XA21-mediated immunity journal April 2010
Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA journal August 1994
A Conserved Threonine Residue in the Juxtamembrane Domain of the XA21 Pattern Recognition Receptor Is Critical for Kinase Autophosphorylation and XA21-mediated Immunity journal January 2010
Plant Innate Immunity: Perception of Conserved Microbial Signatures journal June 2012

Cited By (1)

Four tyrosine residues of the rice immune receptor XA21 are not required for interaction with the co-receptor OsSERK2 or resistance to Xanthomonas oryzae pv. oryzae journal January 2018

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Nuclear localization
Bacterial blight disease
Xanthomonas oryzae pv. oryzae
XA21-mediated immunity