Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser

Demirci, Hasan; Sierra, Raymond G.; Laksmono, Hartawan; Shoeman, Robert L.; Botha, Sabine; Barends, Thomas R.  M.; Nass, Karol; Schlichting, Ilme; Doak, R. Bruce; Gati, Cornelius; Williams, Garth J.; Boutet, Sébastien; Messerschmidt, Marc; Jogl, Gerwald; Dahlberg, Albert E.; Gregory, Steven T.; Bogan, Michael J.

doi:10.1107/s174430911302099x

Title: Serial femtosecond X-ray diffraction of 30S ribosomal subunit microcrystals in liquid suspension at ambient temperature using an X-ray free-electron laser

Journal Article · Mon Aug 19 00:00:00 EDT 2013 · Acta Crystallographica. Section F

DOI:https://doi.org/10.1107/s174430911302099x· OSTI ID:1625815

Demirci, Hasan ^[1]; Sierra, Raymond G. ^[2]; Laksmono, Hartawan ^[2]; Shoeman, Robert L. ^[3]; Botha, Sabine ^[4]; Barends, Thomas R. M. ^[4]; Nass, Karol ^[4]; Schlichting, Ilme ^[4]; Doak, R. Bruce ^[5]; Gati, Cornelius ^[6]; Williams, Garth J. ^[7]; Boutet, Sébastien ^[7]; Messerschmidt, Marc ^[7]; Jogl, Gerwald ^[8]; Dahlberg, Albert E. ^[8]; Gregory, Steven T. ^[8]; Bogan, Michael J. ^[2]

Brown Univ., Providence, RI (United States). Molecular Biology, Cell Biology and Biochemistry; SLAC National Accelerator Lab., Menlo Park, CA (United States). Photon Ultrafast Laser Science and Engineering Inst. (PULSE)
SLAC National Accelerator Lab., Menlo Park, CA (United States). Photon Ultrafast Laser Science and Engineering Inst. (PULSE)
Max-Planck-Institut fur medizinische Forschung, Heidelberg (Germany)
Max-Planck-Institut fur medizinische Forschung, Heidelberg (Germany)
Max-Planck-Institut fur medizinische Forschung, Heidelberg (Germany); Arizona State Univ., Tempe, AZ (United States). Dept. of Physics
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
Brown Univ., Providence, RI (United States). Molecular Biology, Cell Biology and Biochemistry

High-resolution ribosome structures determined by X-ray crystallography have provided important insights into the mechanism of translation. Such studies have thus far relied on large ribosome crystals kept at cryogenic temperatures to reduce radiation damage. Here, the application of serial femtosecond X-ray crystallography (SFX) using an X-ray free-electron laser (XFEL) to obtain diffraction data from ribosome microcrystals in liquid suspension at ambient temperature is described. 30S ribosomal subunit microcrystals diffracted to beyond 6 Å resolution, demonstrating the feasibility of using SFX for ribosome structural studies. The ability to collect diffraction data at near-physiological temperatures promises to provide fundamental insights into the structural dynamics of the ribosome and its functional complexes.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division; USDOE Laboratory Directed Research and Development (LDRD) Program

Grant/Contract Number:: AC02-76SF00515; GM019756; GM094157

OSTI ID:: 1625815

Journal Information:: Acta Crystallographica. Section F, Vol. 69, Issue 9; ISSN 1744-3091

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Structure of the 30S ribosomal decoding complex at ambient temperature Dao, E. Han; Poitevin, Frédéric; Sierra, Raymond G. RNA, Vol. 24, Issue 12 https://doi.org/10.1261/rna.067660.118	journal	August 2018
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