Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation

Martin-Garcia, Jose M.; Conrad, Chelsie E.; Nelson, Garrett; Stander, Natasha; Zatsepin, Nadia A.; Zook, James; Zhu, Lan; Geiger, James; Chun, Eugene; Kissick, David; Hilgart, Mark C.; Ogata, Craig; Ishchenko, Andrii; Nagaratnam, Nirupa; Roy-Chowdhury, Shatabdi; Coe, Jesse; Subramanian, Ganesh; Schaffer, Alexander; James, Daniel; Ketwala, Gihan; Venugopalan, Nagarajan; Xu, Shenglan; Corcoran, Stephen; Ferguson, Dale; Weierstall, Uwe; Spence, John C. H.; Cherezov, Vadim; Fromme, Petra; Fischetti, Robert F.; Liu, Wei

doi:10.1107/S205225251700570X

Serial millisecond crystallography of membrane and soluble protein microcrystals using synchrotron radiation

Journal Article · Wed May 24 00:00:00 EDT 2017 · IUCrJ

DOI:https://doi.org/10.1107/S205225251700570X· OSTI ID:1374440

^[1]; Conrad, Chelsie E. ^[2]; Nelson, Garrett ^[1]; Stander, Natasha ^[1]; Zatsepin, Nadia A. ^[1]; Zook, James ^[1]; Zhu, Lan ^[1]; Geiger, James ^[1]; Chun, Eugene ^[1]; Kissick, David ^[3]; Hilgart, Mark C. ^[3]; Ogata, Craig ^[3]; Ishchenko, Andrii ^[4]; Nagaratnam, Nirupa ^[1]; Roy-Chowdhury, Shatabdi ^[1]; Coe, Jesse ^[1]; Subramanian, Ganesh ^[1]; Schaffer, Alexander ^[1]; ^[5]; Ketwala, Gihan ^[1] more »

Arizona State Univ., Tempe, AZ (United States)
Arizona State Univ., Tempe, AZ (United States); National Cancer Institute, Frederick, MD (United States)
Argonne National Lab. (ANL), Lemont, IL (United States)
Univ. of Southern California, Los Angeles, CA (United States)
Paul Scherrer Inst. (PSI), Villigen (Switzerland)

Crystal structure determination of biological macromolecules using the novel technique of serial femtosecond crystallography (SFX) is severely limited by the scarcity of X-ray free-electron laser (XFEL) sources. However, recent and future upgrades render microfocus beamlines at synchrotron-radiation sources suitable for room-temperature serial crystallography data collection also. Owing to the longer exposure times that are needed at synchrotrons, serial data collection is termed serial millisecond crystallography (SMX). As a result, the number of SMX experiments is growing rapidly, with a dozen experiments reported so far. Here, the first high-viscosity injector-based SMX experiments carried out at a US synchrotron source, the Advanced Photon Source (APS), are reported. Microcrystals (5–20 µm) of a wide variety of proteins, including lysozyme, thaumatin, phycocyanin, the human A_2A adenosine receptor (A_2AAR), the soluble fragment of the membrane lipoprotein Flpp3 and proteinase K, were screened. Crystals suspended in lipidic cubic phase (LCP) or a high-molecular-weight poly(ethylene oxide) (PEO; molecular weight 8 000 000) were delivered to the beam using a high-viscosity injector. In-house data-reduction (hit-finding) software developed at APS as well as the SFX data-reduction and analysis software suites Cheetah and CrystFEL enabled efficient on-site SMX data monitoring, reduction and processing. Complete data sets were collected for A_2AAR, phycocyanin, Flpp3, proteinase K and lysozyme, and the structures of A_2AAR, phycocyanin, proteinase K and lysozyme were determined at 3.2, 3.1, 2.65 and 2.05 Å resolution, respectively. The data demonstrate the feasibility of serial millisecond crystallography from 5–20 µm crystals using a high-viscosity injector at APS. The resolution of the crystal structures obtained in this study was dictated by the current flux density and crystal size, but upcoming developments in beamline optics and the planned APS-U upgrade will increase the intensity by two orders of magnitude. Furthermore, these developments will enable structure determination from smaller and/or weakly diffracting microcrystals.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Science Foundation (NSF); National Institutes of Health (NIH), National Cancer Institute; National Institutes of Health (NIH); USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1374440

Journal Information:: IUCrJ, Journal Name: IUCrJ Journal Issue: 4 Vol. 4; ISSN 2052-2525; ISSN IUCRAJ

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Iterative model building, structure refinement and density modification with the PHENIX AutoBuild wizard. Terwilliger, Thomas C.; Grosse-Kunstleve, Ralf W.; Afonine, Pavel V. International Union of Crystallography (IUCr) https://doi.org/10.17863/cam.52329	journalarticle	January 2008
Serial femtosecond crystallography of soluble proteins in lipidic cubic phase Fromme, Raimund; Ishchenko, Andrii; Metz, Markus Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2015-04280	text	January 2015

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serial millisecond crystallography
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