High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source

Martin-Garcia, Jose M.; Zhu, Lan; Mendez, Derek; Lee, Ming-Yue; Chun, Eugene; Li, Chufeng; Hu, Hao; Subramanian, Ganesh; Kissick, David; Ogata, Craig; Henning, Robert; Ishchenko, Andrii; Dobson, Zachary; Zhang, Shangji; Weierstall, Uwe; Spence, John C. H.; Fromme, Petra; Zatsepin, Nadia A.; Fischetti, Robert F.; Cherezov, Vadim; Liu, Wei

doi:10.1107/S205225251900263X

Title: High-viscosity injector-based pink-beam serial crystallography of microcrystals at a synchrotron radiation source

Journal Article · Fri Apr 05 00:00:00 EDT 2019 · IUCrJ

DOI:https://doi.org/10.1107/S205225251900263X· OSTI ID:1617937

; Zhu, Lan; Mendez, Derek; Lee, Ming-Yue; Chun, Eugene;

; Hu, Hao; Subramanian, Ganesh; Kissick, David; Ogata, Craig; Henning, Robert;

; Dobson, Zachary; Zhang, Shangji; Weierstall, Uwe;

; Fromme, Petra;

;

; Cherezov, Vadim more »

Since the first successful serial crystallography (SX) experiment at a synchrotron radiation source, the popularity of this approach has continued to grow showing that third-generation synchrotrons can be viable alternatives to scarce X-ray free-electron laser sources. Synchrotron radiation flux may be increased ~100 times by a moderate increase in the bandwidth (`pink beam' conditions) at some cost to data analysis complexity. Here, we report the first high-viscosity injector-based pink-beam SX experiments. The structures of proteinase K (PK) and A_2Aadenosine receptor (A_2AAR) were determined to resolutions of 1.8 and 4.2 Å using 4 and 24 consecutive 100 ps X-ray pulse exposures, respectively. Strong PK data were processed using existing Laue approaches, while weaker A_2AAR data required an alternative data-processing strategy. This demonstration of the feasibility presents new opportunities for time-resolved experiments with microcrystals to study structural changes in real time at pink-beam synchrotron beamlines worldwide.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE; National Institutes of Health (NIH), National Institute of General Medical Sciences; National Institutes of Health (NIH), National Cancer Institute; Mayo Clinic; Arizona State University; National Science Foundation (NSF), BioXFEL; National Science Foundation (NSF)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1617937

Alternate ID(s):: OSTI ID: 1542189

Journal Information:: IUCrJ, Journal Name: IUCrJ Vol. 6 Journal Issue: 3; ISSN 2052-2525

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: United Kingdom

Language:: English

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Cited by: 27 works

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Related Subjects

36 MATERIALS SCIENCE
lipidic cubic phase
pink beam
protein dynamics
serial crystallography
time-resolved
third-generation synchrotrons
injector-based serial crystallography
structure determination
membrane proteins
protein structures
X-ray crystallography
structural biology