Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Lawrence, Robert M.; Conrad, Chelsie E.; Zatsepin, Nadia A.; Grant, Thomas D.; Liu, Haiguang; James, Daniel; Nelson, Garrett; Subramanian, Ganesh; Aquila, Andrew; Hunter, Mark S.; Liang, Mengning; Boutet, Sébastien; Coe, Jesse; Spence, John C.  H.; Weierstall, Uwe; Liu, Wei; Fromme, Petra; Cherezov, Vadim; Hogue, Brenda G.

doi:10.1063/1.4929410

Title: Serial femtosecond X-ray diffraction of enveloped virus microcrystals

Journal Article · Thu Aug 20 00:00:00 EDT 2015 · Structural Dynamics

DOI:https://doi.org/10.1063/1.4929410· OSTI ID:1222954

Lawrence, Robert M. ^[1]; Conrad, Chelsie E. ^[1]; Zatsepin, Nadia A. ^[1]; Grant, Thomas D. ^[2]; Liu, Haiguang ^[3]; James, Daniel ^[1]; Nelson, Garrett ^[1]; Subramanian, Ganesh ^[1]; Aquila, Andrew ^[4]; Hunter, Mark S. ^[4]; Liang, Mengning ^[4]; Boutet, Sébastien ^[4]; Coe, Jesse ^[1]; Spence, John C. H. ^[1]; Weierstall, Uwe ^[1]; Liu, Wei ^[1]; Fromme, Petra ^[1]; Cherezov, Vadim ^[5]; Hogue, Brenda G. ^[1]

Arizona State Univ., Tempe, AZ (United States)
State Univ. of New York, Buffalo, NY (United States)
Arizona State Univ., Tempe, AZ (United States); Beijing Computational Science Research Center, Beijing (China)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Univ. of Southern California, Los Angeles, CA (United States)

Serial femtosecond crystallography (SFX) using X-ray free-electron lasers has produced high-resolution, room temperature, time-resolved protein structures. We report preliminary SFX of Sindbis virus, an enveloped icosahedral RNA virus with ~700 Å diameter. Microcrystals delivered in viscous agarose medium diffracted to ~40 Å resolution. Small-angle diffuse X-ray scattering overlaid Bragg peaks and analysis suggests this results from molecular transforms of individual particles. Viral proteins undergo structural changes during entry and infection, which could, in principle, be studied with SFX. This is a pertinent step toward determining room temperature structures from virus microcrystals that may enable time-resolved studies of enveloped viruses.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC03-76SF00515

OSTI ID:: 1222954

Journal Information:: Structural Dynamics, Vol. 2, Issue 4; ISSN 2329-7778

Publisher:: American Crystallographic Association/AIPCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 10 works

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