Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance

Paës, Gabriel; Navarro, David; Benoit, Yves; Blanquet, Senta; Chabbert, Brigitte; Chaussepied, Bernard; Coutinho, Pedro M.; Durand, Sylvie; Grigoriev, Igor V.; Haon, Mireille; Heux, Laurent; Launay, Charlène; Margeot, Antoine; Nishiyama, Yoshiharu; Raouche, Sana; Rosso, Marie-Noëlle; Bonnin, Estelle; Berrin, Jean-Guy

doi:10.1186/s13068-019-1417-8

Title: Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance

Journal Article · Mon Apr 01 00:00:00 EDT 2019 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-019-1417-8· OSTI ID:1560574

Paës, Gabriel ^[1]; Navarro, David ^[2]; Benoit, Yves ^[3]; Blanquet, Senta ^[3]; Chabbert, Brigitte ^[1]; Chaussepied, Bernard ^[3]; Coutinho, Pedro M. ^[4]; Durand, Sylvie ^[5]; Grigoriev, Igor V. ^[6]; Haon, Mireille ^[2]; Heux, Laurent ^[7]; Launay, Charlène ^[5]; Margeot, Antoine ^[3]; Nishiyama, Yoshiharu ^[7]; Raouche, Sana ^[2]; Rosso, Marie-Noëlle ^[2]; Bonnin, Estelle ^[5];

^[2]

Univ. de Reims Champagne-Ardenne, Reims (France). l’Inst. National de Recherche Agronomique (INRA), FARE Lab.
Aix-Marseille Univ., Marseille (France)
IFP Energies Nouvelles, Rueil-Malmaison (France)
Aix-Marseille Univ., and CNRS, Marseille (France)
l’Inst. National de Recherche Agronomique (INRA), Nantes (France)
Univ. of California, Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Univ. Grenoble Alpes, Grenoble (France)

Background:Lignocellulose biomass is known as a recalcitrant material towards enzymatic hydrolysis, increasing the process cost in biorefinery. In nature, filamentous fungi naturally degrade lignocellulose, using an arsenal of hydrolytic and oxidative enzymes. Assessment of enzyme hydrolysis efficiency generally relies on the yield of glucose for a given biomass. To better understand the markers governing recalcitrance to enzymatic degradation, there is a need to enlarge the set of parameters followed during deconstruction. Results:Industrially-pretreated biomass feedstocks from wheat straw, miscanthus and poplar were sequentially hydrolysed following two steps. First, standard secretome from Trichoderma reesei was used to maximize cellulose hydrolysis, producing three recalcitrant lignin-enriched solid substrates. Then fungal secretomes from three basidiomycete saprotrophs (Laetisaria arvalis, Artolenzites elegans and Trametes ljubarskyi) displaying various hydrolytic and oxidative enzymatic profiles were applied to these recalcitrant substrates, and compared to the T. reesei secretome. As a result, most of the glucose was released after the first hydrolysis step. After the second hydrolysis step, half of the remaining glucose amount was released. Overall, glucose yield after the two sequential hydrolyses was more dependent on the biomass source than on the fungal secretomes enzymatic profile. Solid residues obtained after the two hydrolysis steps were characterized using complementary methodologies. Correlation analysis of several physico-chemical parameters showed that released glucose yield was negatively correlated with lignin content and cellulose crystallinity while positively correlated with xylose content and water sorption. Water sorption appears as a pivotal marker of the recalcitrance as it reflects chemical and structural properties of lignocellulosic biomass. Conclusions:Fungal secretomes applied to highly recalcitrant biomass samples can further extend the release of the remaining glucose. The glucose yield can be correlated to chemical and physical markers, which appear to be independent from the biomass type and secretome. Overall, correlations between these markers reveal how nano-scale properties (polymer content and organization) influence macro-scale properties (particle size and water sorption). Further systematic assessment of these markers during enzymatic degradation will foster the development of novel cocktails to unlock the degradation of lignocellulose biomass.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1560574

Journal Information:: Biotechnology for Biofuels, Vol. 12, Issue 1; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 17 works

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MOESM1 of Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance PaĂŤs, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.7936157.v1 The current record is supplemented by this text	text	January 2019
MOESM2 of Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance PaĂŤs, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.7936163 The current record is supplemented by this text	text	January 2019
MOESM2 of Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance PaĂŤs, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.7936163.v1 The current record is supplemented by this text	text	January 2019
MOESM3 of Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance Paës, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.7936172 The current record is supplemented by this text	text	January 2019
MOESM3 of Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance Paës, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.7936172.v1 The current record is supplemented by this text	text	January 2019
MOESM4 of Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance PaĂŤs, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.7936184 The current record is supplemented by this text	text	January 2019
MOESM4 of Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance PaĂŤs, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.7936184.v1 The current record is supplemented by this text	text	January 2019
Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance Paës, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.c.4455497 The current record is supplemented by this collection	collection	January 2019
Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance Paës, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.c.4455497.v1 The current record is supplemented by this collection	collection	January 2019
MOESM1 of Tracking of enzymatic biomass deconstruction by fungal secretomes highlights markers of lignocellulose recalcitrance PaĂŤs, Gabriel; Navarro, David; Benoit, Yves https://doi.org/10.6084/m9.figshare.7936157 The current record is supplemented by this text	text	January 2019