Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery

Yang, Haibing; Zhang, Ximing; Luo, Hao; Liu, Baoyuan; Shiga, Tânia M.; Li, Xu; Kim, Jeong Im; Rubinelli, Peter; Overton, Jonathan C.; Subramanyam, Varun; Cooper, Bruce R.; Mo, Huaping; Abu-Omar, Mahdi M.; Chapple, Clint; Donohoe, Bryon S.; Makowski, Lee; Mosier, Nathan S.; McCann, Maureen C.; Carpita, Nicholas C.; Meilan, Richard

doi:10.1186/s13068-019-1503-y

Title: Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery

Journal Article · Sat Jun 29 00:00:00 EDT 2019 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-019-1503-y· OSTI ID:1618753

Yang, Haibing; Zhang, Ximing; Luo, Hao; Liu, Baoyuan; Shiga, Tânia M.; Li, Xu; Kim, Jeong Im; Rubinelli, Peter; Overton, Jonathan C.; Subramanyam, Varun; Cooper, Bruce R.; Mo, Huaping; Abu-Omar, Mahdi M.; Chapple, Clint; Donohoe, Bryon S.; Makowski, Lee; Mosier, Nathan S.; McCann, Maureen C.; Carpita, Nicholas C.; Meilan, Richard

Low-temperature swelling of cotton linter cellulose and subsequent gelatinization in trifluoroacetic acid (TFA) greatly enhance rates of enzymatic digestion or maleic acid-AlCl₃ catalyzed conversion to hydroxymethylfurfural (HMF) and levulinic acid (LA). However, lignin inhibits low-temperature swelling of TFA-treated intact wood particles from hybrid poplar (Populus tremula x P. alba) and results in greatly reduced yields of glucose or catalytic conversion compared to lignin-free cellulose. Previous studies have established that wood particles from transgenic lines of hybrid poplar with high syringyl (S) lignin content give greater glucose yields following enzymatic digestion. Low-temperature (-20 degrees C) treatment of S-lignin-rich poplar wood particles in TFA slightly increased yields of glucose from enzymatic digestions and HMF and LA from maleic acid-AlCl3 catalysis. Subsequent gelatinization at 55 °C resulted in over 80% digestion of cellulose in only 3 to 6 h with high-S-lignin wood, compared to 20-60% digestion in the wild-type poplar hybrid and transgenic lines high in guaiacyl lignin or 5-hydroxy-G lignin. Disassembly of lignin in woody particles by Ni/C catalytic systems improved yields of glucose by enzymatic digestion or catalytic conversion to HMF and LA. Although lignin was completely removed by Ni/C-catalyzed delignification (CDL) treatment, recalcitrance to enzymatic digestion of cellulose from the high-S lines was reduced compared to other lignin variants. However, cellulose still exhibited considerable recalcitrance to complete enzymatic digestion or catalytic conversion after complete delignification. Low-temperature swelling of the CDL-treated wood particles in TFA resulted in nearly complete enzymatic hydrolysis, regardless of original lignin composition. Genetic modification of lignin composition can enhance the portfolio of aromatic products obtained from lignocellulosic biomass while promoting disassembly into biofuel and bioproduct substrates. CDL enhances rates of enzymatic digestion and chemical conversion, but cellulose remains intrinsically recalcitrant. Cold TFA is sufficient to overcome this recalcitrance after CDL treatment. Our results inform a 'no carbon left behind' strategy to convert total woody biomass into lignin, cellulose, and hemicellulose value streams for the future biorefinery.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: AC36-08GO28308; BEX-10734/13-9

OSTI ID:: 1618753

Alternate ID(s):: OSTI ID: 1544526

Report Number(s):: NREL/JA-2700-74364; 171; PII: 1503

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Vol. 12 Journal Issue: 1; ISSN 1754-6834

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: Netherlands

Language:: English

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Cited by: 29 works

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09 BIOMASS FUELS
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MOESM1 of Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery Yang, Haibing; Zhang, Ximing; Luo, Hao https://doi.org/10.6084/m9.figshare.8378438 The current record is supplemented by this text	text	January 2019
Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery Yang, Haibing; Zhang, Ximing; Luo, Hao https://doi.org/10.6084/m9.figshare.c.4560710 The current record is supplemented by this collection	collection	January 2019
Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery Yang, Haibing; Zhang, Ximing; Luo, Hao https://doi.org/10.6084/m9.figshare.c.4560710.v1 The current record is supplemented by this collection	collection	January 2019
MOESM1 of Overcoming cellulose recalcitrance in woody biomass for the lignin-first biorefinery Yang, Haibing; Zhang, Ximing; Luo, Hao https://doi.org/10.6084/m9.figshare.8378438.v1 The current record is supplemented by this text	text	January 2019