Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population

Ohlsson, Jonas A.; Hallingbäck, Henrik R.; Jebrane, Mohamed; Harman-Ware, Anne E.; Shollenberger, Todd; Decker, Stephen R.; Sandgren, Mats; Rönnberg-Wästljung, Ann-Christin

doi:10.1186/s13068-019-1479-7

Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population

Journal Article · Mon Jun 03 00:00:00 EDT 2019 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-019-1479-7· OSTI ID:1542755

Ohlsson, Jonas A. ^[1]; Hallingbäck, Henrik R. ^[1]; Jebrane, Mohamed ^[1]; Harman-Ware, Anne E. ^[2]; Shollenberger, Todd ^[2]; Decker, Stephen R. ^[2]; Sandgren, Mats ^[1]; ^[1]

Swedish Univ. of Agricultural Sciences, Uppsala (Sweden)
National Renewable Energy Lab. (NREL), Golden, CO (United States)

Background

Salix spp. are high-productivity crops potentially used for lignocellulosic biofuels such as bioethanol. In general, pretreatment is needed to facilitate the enzymatic depolymerization process. Biomass resistance to degradation, i.e., biomass recalcitrance, is a trait which can be assessed by measuring the sugar released after combined pretreatment and enzymatic hydrolysis. We have examined genetic parameters of enzymatic sugar release and other traits related to biorefinery use in a population of 286 natural Salix viminalis clones. Moreover, we have evaluated phenotypic and genetic correlations between these traits and performed a genomewide association mapping analysis using a set of 19,411 markers.

Results

Sugar release (glucose and xylose) after pretreatment and enzymatic saccharification proved highly variable with large genetic and phenotypic variations, and chip heritability estimates (h²) of 0.23-0.29. Lignin syringyl/guaiacyl (S/G) ratio and wood density were the most heritable traits (h² = 0.42 and 0.59, respectively). Sugar release traits were positively correlated, phenotypically and genetically, with biomass yield and lignin S/G ratio. Association mapping revealed seven marker-trait associations below a suggestive significance threshold, including one marker associated with glucose release.

Conclusions

We identified lignin S/G ratio and shoot diameter as heritable traits that could be relatively easily evaluated by breeders, making them suitable proxy traits for developing low-recalcitrance varieties. One marker below the implied threshold for marker associations was identified for sugar release, meriting further investigation while also highlighting the difficulties in employing genomewide association mapping for complex traits.

View Accepted Manuscript (DOE)

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23), Center for Bioenergy Innovation (CBI)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1542755

Report Number(s):: NREL/JA--2700-73918

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 12; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population Ohlsson, Jonas; Hallingbäck, Henrik; Jebrane, Mohamed Figshare https://doi.org/10.6084/m9.figshare.c.4528352.v1	collection	January 2019

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Related Subjects

09 BIOMASS FUELS
Salix viminalis
bioenergy crops
biomass recalcitrance
enzymatic saccharification
genetic parameters
genomewide association study
lignocellulosic biofuels
plant breeding

MOESM1 of Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population Ohlsson, Jonas; Hallingbäck, Henrik; Jebrane, Mohamed https://doi.org/10.6084/m9.figshare.8223185 The current record is supplemented by this text	text	January 2019
MOESM1 of Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population Ohlsson, Jonas; Hallingbäck, Henrik; Jebrane, Mohamed https://doi.org/10.6084/m9.figshare.8223185.v1 The current record is supplemented by this text	text	January 2019
Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population Ohlsson, Jonas; Hallingbäck, Henrik; Jebrane, Mohamed https://doi.org/10.6084/m9.figshare.c.4528352 The current record is supplemented by this collection	collection	January 2019
Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population Ohlsson, Jonas; Hallingbäck, Henrik; Jebrane, Mohamed https://doi.org/10.6084/m9.figshare.c.4528352.v1 The current record is supplemented by this collection	collection	January 2019

Genetic variation of biomass recalcitrance in a natural Salix viminalis (L.) population

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