Ebola virus VP30 and nucleoprotein interactions modulate viral RNA synthesis
- Guangzhou Medical Univ. (China); Washington Univ. School of Medicine, St. Louis, MO (United States)
- Georgia State Univ., Atlanta, GA (United States)
- Washington Univ. School of Medicine, St. Louis, MO (United States)
Ebola virus (EBOV) is an enveloped negative-sense RNA virus that causes sporadic outbreaks with high case fatality rates. Ebola viral protein 30 (eVP30) plays a critical role in EBOV transcription initiation at the nucleoprotein (eNP) gene, with additional roles in the replication cycle such as viral assembly. However, the mechanistic basis for how eVP30 functions during the virus replication cycle is currently unclear. Here we define a key interaction between eVP30 and a peptide derived from eNP that is important to facilitate interactions leading to the recognition of the RNA template. We present crystal structures of the eVP30 C-terminus in complex with this eNP peptide. Functional analyses of the eVP30–eNP interface identify residues that are critical for viral RNA synthesis. Altogether, these results support a model where the eVP30–eNP interaction plays a critical role in transcription initiation and provides a novel target for the development of antiviral therapy.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Organization:
- USDOE; National Institutes of Health (NIH); Dept. of the Defense, Defense Threat Reduction Agency
- Grant/Contract Number:
- AC02-06CH11357; R01AI107056; R01AI123926; P01AI120943; R01AI114654; U191099565; U19AI109945; U19AI109664; HDTRA1-14-0013; HDTRA1-12-1-0051
- OSTI ID:
- 1368315
- Journal Information:
- Nature Communications, Vol. 8, Issue 1; ISSN 2041-1723
- Publisher:
- Nature Publishing GroupCopyright Statement
- Country of Publication:
- United States
- Language:
- ENGLISH
Web of Science
Conformational Selection of a Polyproline Peptide by Ebola Virus VP30
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Host factor SMYD3 is recruited by Ebola virus nucleoprotein to facilitate viral mRNA transcription
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journal | January 2019 |
Serine-Arginine Protein Kinase 1 Regulates Ebola Virus Transcription
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journal | February 2020 |
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