Suppression of innate immune responses during filoviral infection contributes to disease severity. Ebola (EBOV) and Marburg (MARV) viruses each encode a VP35 protein that suppresses RIG-I-like receptor signaling and interferon-α/β (IFN-α/β) production by several mechanisms, including direct binding to double stranded RNA (dsRNA). Here, we demonstrate that in cell culture, MARV infection results in a greater upregulation of IFN responses as compared to EBOV infection. This correlates with differences in the efficiencies by which EBOV and MARV VP35s antagonize RIG-I signaling. Furthermore, structural and biochemical studies suggest that differential recognition of RNA elements by the respective VP35 C-terminal IFN inhibitory domain (IID) rather than affinity for RNA by the respective VP35s is critical for this observation. Our studies reveal functional differences in EBOV versus MARV VP35 RNA binding that result in unexpected differences in the host response to deadly viral pathogens.
Edwards, Megan R., Liu, Gai, Mire, Chad E., Sureshchandra, Suhas, Luthra, Priya, Yen, Benjamin, Shabman, Reed S., Leung, Daisy W., Messaoudi, Ilhem, Geisbert, Thomas W., Amarasinghe, Gaya K., & Basler, Christopher F. (2016). Differential Regulation of Interferon Responses by Ebola and Marburg Virus VP35 Proteins. Cell Reports, 14(7). https://doi.org/10.1016/j.celrep.2016.01.049
Edwards, Megan R., Liu, Gai, Mire, Chad E., et al., "Differential Regulation of Interferon Responses by Ebola and Marburg Virus VP35 Proteins," Cell Reports 14, no. 7 (2016), https://doi.org/10.1016/j.celrep.2016.01.049
@article{osti_1351395,
author = {Edwards, Megan R. and Liu, Gai and Mire, Chad E. and Sureshchandra, Suhas and Luthra, Priya and Yen, Benjamin and Shabman, Reed S. and Leung, Daisy W. and Messaoudi, Ilhem and Geisbert, Thomas W. and others},
title = {Differential Regulation of Interferon Responses by Ebola and Marburg Virus VP35 Proteins},
annote = {Suppression of innate immune responses during filoviral infection contributes to disease severity. Ebola (EBOV) and Marburg (MARV) viruses each encode a VP35 protein that suppresses RIG-I-like receptor signaling and interferon-α/β (IFN-α/β) production by several mechanisms, including direct binding to double stranded RNA (dsRNA). Here, we demonstrate that in cell culture, MARV infection results in a greater upregulation of IFN responses as compared to EBOV infection. This correlates with differences in the efficiencies by which EBOV and MARV VP35s antagonize RIG-I signaling. Furthermore, structural and biochemical studies suggest that differential recognition of RNA elements by the respective VP35 C-terminal IFN inhibitory domain (IID) rather than affinity for RNA by the respective VP35s is critical for this observation. Our studies reveal functional differences in EBOV versus MARV VP35 RNA binding that result in unexpected differences in the host response to deadly viral pathogens.},
doi = {10.1016/j.celrep.2016.01.049},
url = {https://www.osti.gov/biblio/1351395},
journal = {Cell Reports},
issn = {ISSN 2211-1247},
number = {7},
volume = {14},
place = {United States},
publisher = {Elsevier},
year = {2016},
month = {02}}