Biosynthesis of prostaglandins in gingiva of patients with chronic periodontitis
This study was undertaken to determine the ability of inflamed and normal gingival tissues to synthesize prostaglandins (PGs) from the precursor arachidonic acid. Thirteen samples of inflamed human gingival tissue and six samples of normal human gingival tissue were studied. The inflammation was characterized histologically. After incubation of the tissue with (/sup 14/C)arachidonate, PG metabolites were separated by thin-layer chromatography and identified by comparison with co-chromatographed standards. Inflamed gingival tissue synthesized significantly larger amounts, compared to normal tissue, of 6-keto-PGF1 alpha (P less than 0.05), thromboxane B2, PGD2, and PGA2. Some unidentified metabolites, possibly lipoxygenase products were detected in significantly larger amounts in inflamed than in normal tissue.
- Research Organization:
- Mayo Clinic, Rochester, MN
- OSTI ID:
- 5480675
- Journal Information:
- J. Periodontol.; (United States), Vol. 56:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ARACHIDONIC ACID
METABOLISM
ORAL CAVITY
INFLAMMATION
PROSTAGLANDINS
BIOSYNTHESIS
THIN-LAYER CHROMATOGRAPHY
CARBON 14 COMPOUNDS
MEN
PATHOLOGICAL CHANGES
TRACER TECHNIQUES
ANIMALS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MALES
MAMMALS
MAN
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
SEPARATION PROCESSES
SYMPTOMS
SYNTHESIS
VERTEBRATES
550901* - Pathology- Tracer Techniques