The effect of non-steroidal anti-inflammatory drugs on the metabolism of /sup 14/C-arachidonic acid by human gingival tissue in vitro

Journal Article · Thu Sep 01 00:00:00 EDT 1983 · J. Dent. Res.; (United States)

OSTI ID:5606884

Elattar, T M; Lin, H S; Tira, D E

We investigated the effect of non-steroidal anti-inflammatory drugs on prostaglandins (PGs) and 12-hydroxyeicosatetraenoic acid (12-HETE) formation by inflamed human gingival tissues. Gingival tissue homogenates were incubated with /sup 14/C-arachidonic acid in the presence of indomethacin, piroxicam, or ibuprofen, and the organic solvent extracts were chromatographed on silica gel plates with standards for radiometric assay. There was a significant negative trend between the doses (10(-7)-10(-3) M) of each of indomethacin, piroxicam, and ibuprofen, and the amounts of PGF2 alpha, PGE2, PGD2, and 15-keto-PGE2 produced. All three drugs have a significant inhibitory effect on PGs and 12-HETE production at 10(-3) M when compared with the control. The rank order effectiveness of the drugs, at 10(-3) M, on PG inhibition was indomethacin greater than piroxicam greater than ibuprofen, and on 12-HETE inhibition was indomethacin greater than ibuprofen greater than piroxicam.

• Research Organization: Laboratory of Hormone Research, University of Missouri, School of Dentistry, Kansas City
• OSTI ID: 5606884
• Journal Information: J. Dent. Res.; (United States), Vol. 62:9
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTIPYRETICS
BIOLOGICAL EFFECTS
COMPARATIVE EVALUATIONS
ARACHIDONIC ACID
METABOLISM
ORAL CAVITY
INFLAMMATION
PROSTAGLANDINS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
IN VITRO
INHIBITION
MAN
TRACER TECHNIQUES
ANIMALS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DIGESTIVE SYSTEM
DRUGS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PRIMATES
SYMPTOMS
SYNTHESIS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques