The effect of non-steroidal anti-inflammatory drugs on the metabolism of /sup 14/C-arachidonic acid by human gingival tissue in vitro
We investigated the effect of non-steroidal anti-inflammatory drugs on prostaglandins (PGs) and 12-hydroxyeicosatetraenoic acid (12-HETE) formation by inflamed human gingival tissues. Gingival tissue homogenates were incubated with /sup 14/C-arachidonic acid in the presence of indomethacin, piroxicam, or ibuprofen, and the organic solvent extracts were chromatographed on silica gel plates with standards for radiometric assay. There was a significant negative trend between the doses (10(-7)-10(-3) M) of each of indomethacin, piroxicam, and ibuprofen, and the amounts of PGF2 alpha, PGE2, PGD2, and 15-keto-PGE2 produced. All three drugs have a significant inhibitory effect on PGs and 12-HETE production at 10(-3) M when compared with the control. The rank order effectiveness of the drugs, at 10(-3) M, on PG inhibition was indomethacin greater than piroxicam greater than ibuprofen, and on 12-HETE inhibition was indomethacin greater than ibuprofen greater than piroxicam.
- Research Organization:
- Laboratory of Hormone Research, University of Missouri, School of Dentistry, Kansas City
- OSTI ID:
- 5606884
- Journal Information:
- J. Dent. Res.; (United States), Journal Name: J. Dent. Res.; (United States) Vol. 62:9; ISSN JDREA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ANTIPYRETICS
ARACHIDONIC ACID
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
COMPARATIVE EVALUATIONS
DIGESTIVE SYSTEM
DRUGS
IN VITRO
INFLAMMATION
INHIBITION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MAN
METABOLISM
MONOCARBOXYLIC ACIDS
ORAL CAVITY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PRIMATES
PROSTAGLANDINS
SYMPTOMS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES