Lipoxygenase- and cyclooxygenase-reaction products and incorporation into glycerolipids or radiolabeled arachidonic acid in the bovine retina

Journal Article · Wed Feb 01 04:00:00 EST 1984 · Prostaglandins; (United States)

DOI:https://doi.org/10.1016/0090-6980(84)90074-1· OSTI ID:6749733

Birkle, D L; Bazan, N G

The metabolism of radiolabeled arachidonic acid (AA) by the intact bovine retina in vitro has been studied. Synthesis of prostaglandins (PGs) and hydroxyeicosatetraenoic acids (HETEs), and incorporation of AA into glycerolipids has been measured by reverse-phase and straight-phase high performance liquid chromatography with flow scintillation detection, and by thin-layer chromatography. AA was actively acylated into glycerolipids, particularly triglycerides, phosphatidylcholine and phosphatidylinositol. AA was also converted to the major PGs, PGF2 alpha, PGE2, PGD2, 6-keto-PGF1 alpha and TXB2, and to the lipoxygenase reaction products, 12-HETE, 5-HETE, and other monohydroxy isomers. Approximately 6% of the radiolabeled AA was converted to eicosanoids. The synthesis of HETEs was inhibited in a concentration-dependent manner (IC50 . 8.3 nM) by nordihydroguaiaretic acid (NDGA). PG synthesis was inhibited by aspirin (10 microM), indomethacin (1 microM) and NDGA (IC50 . 380 nM). Metabolism of AA via lipoxygenase, cyclooxygenase and activation-acylation was inhibited by boiling retinal tissue prior to incubation. These studies demonstrate an active system for the uptake and utilization of AA in the bovine retina, and provide the first evidence of lipoxygenase-mediated metabolism of AA, resulting in the synthesis of mono-hydroxyeicosatetraenoic acids, in the retina.

• Research Organization: LSU Eye Center, Louisiana State University Medical Center, New Orleans
• OSTI ID: 6749733
• Journal Information: Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 27:2; ISSN PRGLB
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ARACHIDONIC ACID
BIOSYNTHESIS
BODY
BODY AREAS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ENZYMES
EYES
FACE
HEAD
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PROSTAGLANDINS
RETINA
SENSE ORGANS
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES