Lipoxygenase products mediate the attachment of rat macrophages to glomeruli in vitro

Journal Article · Sat Jun 01 04:00:00 EDT 1985 · Kidney Int.; (United States)

DOI:https://doi.org/10.1038/ki.1985.92· OSTI ID:5127948

Baud, L; Sraer, J; Delarue, F; Bens, M; Balavoine, F; Schlondorff, D; Ardaillou, R; Sraer, J D

Because there is an accumulation of macrophages in the Bowman's space during human and experimental glomerulonephritis, the authors have studied the binding of (/sup 3/H)-uridine labeled macrophages to isolated glomeruli. Binding was related to the glomerular protein and macrophage concentrations, temperature, time of incubation, and was a saturable process. Macrophage adherence depended on glomerular lipoxygenase activity but not on glomerular cyclooxygenase activity since preincubation of glomeruli with nordihydroguaiaretic acid (NDGA) inhibited this phenomenon whereas preincubation with indomethacin was ineffective. Glomeruli interacted with macrophages in converting arachidonic acid (C20:4) to prostaglandins (PG) since productions of 6 keto-PGF1 alpha, TXB2, and PGD2 by glomeruli and macrophages incubated in combination were much greater than the sums of their respective productions by glomeruli and macrophages incubated separately. Macrophages were the source of the supplementary synthesis of PG which was abolished when these cells were pretreated with aspirin. Stimulation of macrophages by glomeruli was blunted by pretreatment of glomeruli with NDGA. Production of PG and of 12-HETE by macrophages was stimulated by a lipid extract of glomeruli containing the oxygenated metabolites of C20:4. Direct addition of 12-HPETE also stimulated macrophage functions. These data suggest that macrophage attachment to glomeruli and macrophage stimulation in the presence of glomeruli depend on glomerular lipoxygenase activity.

• Research Organization: Tenon Hospital, Paris, France
• OSTI ID: 5127948
• Journal Information: Kidney Int.; (United States), Journal Name: Kidney Int.; (United States) Vol. 6; ISSN KDYIA
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
ANTIPYRETICS
ARACHIDONIC ACID
AZINES
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CHEMICAL BONDS
CONNECTIVE TISSUE CELLS
DRUGS
ENZYME ACTIVITY
ENZYMES
GLOMERULI
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KIDNEYS
KINETICS
LABELLED COMPOUNDS
MACROPHAGES
MAMMALS
MONOCARBOXYLIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PHAGOCYTES
PROSTAGLANDINS
PYRIMIDINES
RADIOASSAY
RADIOIMMUNOASSAY
RATS
REACTION KINETICS
RIBOSIDES
RODENTS
SOMATIC CELLS
SYNTHESIS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
URACILS
URIDINE
VERTEBRATES