Synthesis of tritium-labeled 12-({sup 3}H)HPETE and 12-({sup 3}H)HPETE using 12-lipoxygenase
Journal Article
·
· Radiochemistry
OSTI ID:171786
- Inst. of Molecular Genetics, Moscow (Russian Federation); and others
12-({sup 3}H)HPETE was synthesized by oxidation of ({sup 3}H)arachiodonic acid with a preparation of 12-lipoxygenase isolated and partially purified from human thrombocytes. Inhibition of peroxidase activity of the 12-lipoxygenase with indomethacin allowed us to prepare 12-({sup 3}H)PETE. The yields of 12-({sup 3}H)HPETE and 12-({sup 3}H)HETE were as high as 30-40 and 40-50% respectively. The molar radioactivity of these preparations was 140-142 Ci mmol{sup {minus}1}.
- OSTI ID:
- 171786
- Journal Information:
- Radiochemistry, Journal Name: Radiochemistry Journal Issue: 3 Vol. 37; ISSN RDIOEO; ISSN 1066-3622
- Country of Publication:
- United States
- Language:
- English
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