Biosynthesis of prostaglandins in gingiva of patients with chronic periodontitis

Journal Article · Tue Jan 01 04:00:00 EST 1985 · J. Periodontol.; (United States)

DOI:https://doi.org/10.1902/jop.1985.56.1.44· OSTI ID:5480675

Mendieta, C F; Reeve, C M; Romero, J C

This study was undertaken to determine the ability of inflamed and normal gingival tissues to synthesize prostaglandins (PGs) from the precursor arachidonic acid. Thirteen samples of inflamed human gingival tissue and six samples of normal human gingival tissue were studied. The inflammation was characterized histologically. After incubation of the tissue with (/sup 14/C)arachidonate, PG metabolites were separated by thin-layer chromatography and identified by comparison with co-chromatographed standards. Inflamed gingival tissue synthesized significantly larger amounts, compared to normal tissue, of 6-keto-PGF1 alpha (P less than 0.05), thromboxane B2, PGD2, and PGA2. Some unidentified metabolites, possibly lipoxygenase products were detected in significantly larger amounts in inflamed than in normal tissue.

• Research Organization: Mayo Clinic, Rochester, MN
• OSTI ID: 5480675
• Journal Information: J. Periodontol.; (United States), Journal Name: J. Periodontol.; (United States) Vol. 56:1; ISSN JOPRA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CHROMATOGRAPHY
DIGESTIVE SYSTEM
INFLAMMATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MALES
MAMMALS
MAN
MEN
METABOLISM
MONOCARBOXYLIC ACIDS
ORAL CAVITY
ORGANIC ACIDS
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PRIMATES
PROSTAGLANDINS
SEPARATION PROCESSES
SYMPTOMS
SYNTHESIS
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
VERTEBRATES