Effects of ozone on lung mechanics and cyclooxygenase metabolites in dogs

Journal Article · Tue Oct 01 04:00:00 EDT 1991 · Prostaglandins; (United States)

DOI:https://doi.org/10.1016/0090-6980(91)90083-R· OSTI ID:5307113

Fouke, J M; Wolin, A D; McFadden, Jr, E R ^[1]

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1. Department of Biomedical Engineering, Case Western Reserve University, Cleveland, OH (United States)

To determine if acute exposure to ozone can cause changes in the production of cyclooxygenase metabolites of arachidonic acid (AA) in the lung which are associated with changes in lung mechanics, we exposed mongrel dogs to 0.5 ppm ozone for two hours. We measured pulmonary resistance (RL) and dynamic compliance (Cdyn) and obtained methacholine dose response curves and bronchoalveolar lavagate (BAL) before and after the exposures. We calculated the provocative dose of methacholine necessary to increase RL 50% (PD50) and analyzed the BAL for four cyclooxygenase metabolites of AA: a stable hydrolysis product of prostacyclin, 6-keto-prostaglandin F1 alpha (6-keto-PgF1 alpha); prostaglandin E2 (PgE2); a stable hydrolysis product of thromboxane A2, thromboxane B2 (TxB2); and prostaglandin F2 alpha (PgF2 alpha). Following ozone exposure, RL increased from 4.75 +/- 1.06 to 6.08 +/- 1.3 cm H2O/L/sec (SEM) (p less than 0.05), Cdyn decreased from 0.0348 +/- 0.0109 TO .0217 +/- .0101 L/cm H2O (p less than 0.05), and PD50 decreased from 4.32 +/- 2.41 to 0.81 +/- 0.49 mg/cc (p less than 0.05). The baseline metabolite levels were as follows: 6-keto PgF1 alpha: 96.1 +/- 28.8 pg/ml; PgE2: 395.8 +/- 67.1 pg/ml; TxB2: 48.5 +/- 11.1 pg/ml; PgF2 alpha: 101.5 +/- 22.6 pg/ml. Ozone had no effect on any of these prostanoids. These studies quantify the magnitude of cyclooxygenase products of AA metabolism in BAL from dog lungs and demonstrate that changes in their levels are not prerequisites for ozone-induced changes in lung mechanics or airway reactivity.

• OSTI ID: 5307113
• Journal Information: Prostaglandins; (United States), Journal Name: Prostaglandins; (United States) Vol. 42:4; ISSN PRGLB; ISSN 0090-6980
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACUTE EXPOSURE
ANIMALS
ARACHIDONIC ACID
BIOSYNTHESIS
BODY
CARBOXYLIC ACIDS
DOGS
DOSE-RESPONSE RELATIONSHIPS
ENZYMES
LAVAGE
LUNGS
MAMMALS
METABOLISM
METABOLITES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
OZONE
PATHOLOGICAL CHANGES
PROSTAGLANDINS
PROTEINS
RESPIRATORY SYSTEM
SYNTHESIS
TOXICITY
VERTEBRATES