Microwave irradiation is effective in the rapid fixation of gastric mucosa for determination of the prostaglandin content

Journal Article · Sat Dec 01 04:00:00 EST 1990 · Journal of Pharmacological Methods; (USA)

OSTI ID:5921777

Ueda, F; Watanabe, M; Hirata, Y; Kimura, K ^[1]

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1. Nippon Shinyaku Co., Ltd., Kyoto (Japan)

The gastric mucosal content of prostaglandin (PG) E2, 6-keto PGF1 alpha, and thromboxane (TX) B2 was determined by radioimmunoassay in unfed rats. The stomach was removed, and gastric mucosal specimens were prepared by microwave irradiation of the (1) intact stomach, (2) frozen stomach, (3) separated frozen glandular portion, (4) frozen gastric mucosa separated from the stomach wall, and (5) separation of frozen gastric mucosa with no microwave irradiation. Procedure 1 resulted in PGE2, TXB2, and 6-keto PGF1 alpha levels of 0.75, 2.33, and 3.04 ng/g tissue in the fundus, and 0.33, 1.92, and 1.76 ng/g tissue in the antrum, respectively. In procedures 2-4 these values were much higher, indicating that the PG content of the gastric mucosa is liable to be changed by various artificial procedures, such as freezing and surgical and mechanical handling. In procedure 5, values of PG contents were quite unreliable. These results suggest that microwave irradiation immediately after removal of the stomach may be the most reliable procedure for the accurate determination of the PG content of the gastric mucosa in vivo.

• OSTI ID: 5921777
• Journal Information: Journal of Pharmacological Methods; (USA), Journal Name: Journal of Pharmacological Methods; (USA) Vol. 24:4; ISSN 0160-5402; ISSN JPMED
• Country of Publication: United States
• Language: English

Related Subjects

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560400* -- Other Environmental Pollutant Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
BIOASSAY
BIOLOGICAL EFFECTS
BIOSYNTHESIS
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
ELECTROMAGNETIC RADIATION
GASTROINTESTINAL TRACT
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MAMMALS
MEMBRANES
MICROWAVE RADIATION
MUCOUS MEMBRANES
PROSTAGLANDINS
RADIATIONS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
RATS
RODENTS
SAMPLE PREPARATION
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES