Steam explosion pretreatment coupling high-temperature short-time sterilization facilitating cellulose degradation and sporulation-regulatory gene expression in high-solid fermentation
Abstract
Steam explosion coupling high-temperature short-time sterilization (SE-HTST) was exploited to modify cellulosic biomass medium properties and promote high-solid fermentation (HSF). Biomass characterization analysis showed that SE-HTST enlarged microstructural pores and cavities in solid media, providing more effective space for microbial growth. Meanwhile, SE-HTST helped to release glucose from the cellulose with 35.8 ± 4.5, 20.0 ± 2.3, and 12.3 ± 5.7 mg glucose/g dry medium at 24, 48, and 72 h of fermentation, which were 3.1, 2.3, and 1.5 times higher than that in medium from conventional thermal sterilization (CTS), respectively. SE-HTST increased the viable cell and spore number of Bacillus subtilis by 1.8 and 1.6 times at 72 h of fermentation compared to CTS. Moreover, the expressions of master transcriptional gene spo0A and the early sigma factors of sigF and sigE genes gradually increased in the SE-HTST medium, showing enhanced sporulation in HSF. Therefore, SE-HTST is an effective strategy for facilitating cellulose degradation, improving glucose nutrients in biomass medium, and promoting sporulation-regulatory gene expression during high-solid fermentation, which enhances the production of microbial ecological agents using B. subtilis significantly.
- Authors:
-
[5]
- Inner Mongolia Univ., Hohhot (China)
- Tianjin Univ. (China)
- Univ. of Tennessee, Knoxville, TN (United States)
- Inner Mongolia Univ., Hohhot (China); Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)
- Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1985376
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- International Journal of Biological Macromolecules
- Additional Journal Information:
- Journal Volume: 232; Journal Issue: 1; Journal ID: ISSN 0141-8130
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; cellulosic biomass; cellulose; steam explosion; high-solid fermentation; sporulation; Bacillus subtilis
Citation Formats
Li, Chonglei, Du, Xiaoyu, Liu, Zhi-Hua, Li, Bing-Zhi, Meng, Xianzhi, Zhao, Ji, Zhao, Zhi-Min, and Ragauskas, Arthur J. Steam explosion pretreatment coupling high-temperature short-time sterilization facilitating cellulose degradation and sporulation-regulatory gene expression in high-solid fermentation. United States: N. p., 2023.
Web. doi:10.1016/j.ijbiomac.2023.123475.
Li, Chonglei, Du, Xiaoyu, Liu, Zhi-Hua, Li, Bing-Zhi, Meng, Xianzhi, Zhao, Ji, Zhao, Zhi-Min, & Ragauskas, Arthur J. Steam explosion pretreatment coupling high-temperature short-time sterilization facilitating cellulose degradation and sporulation-regulatory gene expression in high-solid fermentation. United States. https://doi.org/10.1016/j.ijbiomac.2023.123475
Li, Chonglei, Du, Xiaoyu, Liu, Zhi-Hua, Li, Bing-Zhi, Meng, Xianzhi, Zhao, Ji, Zhao, Zhi-Min, and Ragauskas, Arthur J. Sat .
"Steam explosion pretreatment coupling high-temperature short-time sterilization facilitating cellulose degradation and sporulation-regulatory gene expression in high-solid fermentation". United States. https://doi.org/10.1016/j.ijbiomac.2023.123475. https://www.osti.gov/servlets/purl/1985376.
@article{osti_1985376,
title = {Steam explosion pretreatment coupling high-temperature short-time sterilization facilitating cellulose degradation and sporulation-regulatory gene expression in high-solid fermentation},
author = {Li, Chonglei and Du, Xiaoyu and Liu, Zhi-Hua and Li, Bing-Zhi and Meng, Xianzhi and Zhao, Ji and Zhao, Zhi-Min and Ragauskas, Arthur J.},
abstractNote = {Steam explosion coupling high-temperature short-time sterilization (SE-HTST) was exploited to modify cellulosic biomass medium properties and promote high-solid fermentation (HSF). Biomass characterization analysis showed that SE-HTST enlarged microstructural pores and cavities in solid media, providing more effective space for microbial growth. Meanwhile, SE-HTST helped to release glucose from the cellulose with 35.8 ± 4.5, 20.0 ± 2.3, and 12.3 ± 5.7 mg glucose/g dry medium at 24, 48, and 72 h of fermentation, which were 3.1, 2.3, and 1.5 times higher than that in medium from conventional thermal sterilization (CTS), respectively. SE-HTST increased the viable cell and spore number of Bacillus subtilis by 1.8 and 1.6 times at 72 h of fermentation compared to CTS. Moreover, the expressions of master transcriptional gene spo0A and the early sigma factors of sigF and sigE genes gradually increased in the SE-HTST medium, showing enhanced sporulation in HSF. Therefore, SE-HTST is an effective strategy for facilitating cellulose degradation, improving glucose nutrients in biomass medium, and promoting sporulation-regulatory gene expression during high-solid fermentation, which enhances the production of microbial ecological agents using B. subtilis significantly.},
doi = {10.1016/j.ijbiomac.2023.123475},
journal = {International Journal of Biological Macromolecules},
number = 1,
volume = 232,
place = {United States},
year = {Sat Jan 28 00:00:00 EST 2023},
month = {Sat Jan 28 00:00:00 EST 2023}
}
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