The Clostridium Sporulation Programs: Diversity and Preservation of Endospore Differentiation
Abstract
Bacillus and Clostridium organisms initiate the sporulation process when unfavorable conditions are detected. The sporulation process is a carefully orchestrated cascade of events at both the transcriptional and posttranslational levels involving a multitude of sigma factors, transcription factors, proteases, and phosphatases. Like Bacillus genomes, sequenced Clostridium genomes contain genes for all major sporulation-specific transcription and sigma factors (spo0A, sigH, sigF, sigE, sigG, and sigK) that orchestrate the sporulation program. However, recent studies have shown that there are substantial differences in the sporulation programs between the two genera as well as among different Clostridium species. First, in the absence of a Bacillus-like phosphorelay system, activation of Spo0A in Clostridium organisms is carried out by a number of orphan histidine kinases. Second, downstream of Spo0A, the transcriptional and posttranslational regulation of the canonical set of four sporulation-specific sigma factors (σF, σE, σG, and σK) display different patterns, not only compared to Bacillus but also among Clostridium organisms. Finally, recent studies demonstrated that σK, the last sigma factor to be activated according to the Bacillus subtilis model, is involved in the very early stages of sporulation in Clostridium acetobutylicum, C. perfringens, and C. botulinum as well as in the very late stages ofmore »
- Authors:
-
- Univ. of Delaware, Newark, DE (United States); Sultan Qaboos Univ., Muscat (Oman)
- Elcriton Inc., New Castle, DE (United States)
- Elcriton Inc., New Castle, DE (United States); Univ. of Delaware, Newark, DE (United States)
- Publication Date:
- Research Org.:
- Univ. of Delaware, Newark, DE (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1454688
- Grant/Contract Number:
- sc0007092
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Microbiology and Molecular Biology Reviews
- Additional Journal Information:
- Journal Volume: 79; Journal Issue: 1; Journal ID: ISSN 1092-2172
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Al-Hinai, Mohab A., Jones, Shawn W., and Papoutsakis, Eleftherios T. The Clostridium Sporulation Programs: Diversity and Preservation of Endospore Differentiation. United States: N. p., 2015.
Web. doi:10.1128/MMBR.00025-14.
Al-Hinai, Mohab A., Jones, Shawn W., & Papoutsakis, Eleftherios T. The Clostridium Sporulation Programs: Diversity and Preservation of Endospore Differentiation. United States. https://doi.org/10.1128/MMBR.00025-14
Al-Hinai, Mohab A., Jones, Shawn W., and Papoutsakis, Eleftherios T. Wed .
"The Clostridium Sporulation Programs: Diversity and Preservation of Endospore Differentiation". United States. https://doi.org/10.1128/MMBR.00025-14. https://www.osti.gov/servlets/purl/1454688.
@article{osti_1454688,
title = {The Clostridium Sporulation Programs: Diversity and Preservation of Endospore Differentiation},
author = {Al-Hinai, Mohab A. and Jones, Shawn W. and Papoutsakis, Eleftherios T.},
abstractNote = {Bacillus and Clostridium organisms initiate the sporulation process when unfavorable conditions are detected. The sporulation process is a carefully orchestrated cascade of events at both the transcriptional and posttranslational levels involving a multitude of sigma factors, transcription factors, proteases, and phosphatases. Like Bacillus genomes, sequenced Clostridium genomes contain genes for all major sporulation-specific transcription and sigma factors (spo0A, sigH, sigF, sigE, sigG, and sigK) that orchestrate the sporulation program. However, recent studies have shown that there are substantial differences in the sporulation programs between the two genera as well as among different Clostridium species. First, in the absence of a Bacillus-like phosphorelay system, activation of Spo0A in Clostridium organisms is carried out by a number of orphan histidine kinases. Second, downstream of Spo0A, the transcriptional and posttranslational regulation of the canonical set of four sporulation-specific sigma factors (σF, σE, σG, and σK) display different patterns, not only compared to Bacillus but also among Clostridium organisms. Finally, recent studies demonstrated that σK, the last sigma factor to be activated according to the Bacillus subtilis model, is involved in the very early stages of sporulation in Clostridium acetobutylicum, C. perfringens, and C. botulinum as well as in the very late stages of spore maturation in C. acetobutylicum. Despite profound differences in initiation, propagation, and orchestration of expression of spore morphogenetic components, these findings demonstrate not only the robustness of the endospore sporulation program but also the plasticity of the program to generate different complex phenotypes, some apparently regulated at the epigenetic level.},
doi = {10.1128/MMBR.00025-14},
journal = {Microbiology and Molecular Biology Reviews},
number = 1,
volume = 79,
place = {United States},
year = {Wed Jan 28 00:00:00 EST 2015},
month = {Wed Jan 28 00:00:00 EST 2015}
}
Web of Science
Works referenced in this record:
The identification of four histidine kinases that influence sporulation in Clostridium thermocellum
journal, August 2014
- Mearls, Elizabeth B.; Lynd, Lee R.
- Anaerobe, Vol. 28
Control of the Expression and Compartmentalization of G Activity during Sporulation of Bacillus subtilis by Regulators of F and E
journal, September 2005
- Chary, V. K.; Meloni, M.; Hilbert, D. W.
- Journal of Bacteriology, Vol. 187, Issue 19
Clostridia: the importance of their exceptional substrate and metabolite diversity for biofuel and biorefinery applications
journal, June 2012
- Tracy, Bryan P.; Jones, Shawn W.; Fast, Alan G.
- Current Opinion in Biotechnology, Vol. 23, Issue 3
Localization of a second SigH promoter in the Bacillus subtilis sigA operon and regulation of dnaE expression by the promoter.
journal, October 1990
- Qi, F. X.; Doi, R. H.
- Journal of Bacteriology, Vol. 172, Issue 10
Fermentative production of butanol—the industrial perspective
journal, June 2011
- Green, Edward M.
- Current Opinion in Biotechnology, Vol. 22, Issue 3
Multiple orphan histidine kinases interact directly with Spo0A to control the initiation of endospore formation in Clostridium acetobutylicum: Sporulation initiation in clostridia
journal, March 2011
- Steiner, Elisabeth; Dago, Angel E.; Young, Danielle I.
- Molecular Microbiology, Vol. 80, Issue 3
Sigma factor and sporulation genes in Clostridium
journal, October 1995
- Sauer, Uwe; Santangelo, Joseph D.; Treuner, Anke
- FEMS Microbiology Reviews, Vol. 17, Issue 3
The genome sequence of Clostridium tetani, the causative agent of tetanus disease
journal, January 2003
- Bruggemann, H.; Baumer, S.; Fricke, W. F.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 3
Involvement of Clostridium botulinum ATCC 3502 Sigma Factor K in Early-Stage Sporulation
journal, April 2012
- Kirk, David G.; Dahlsten, Elias; Zhang, Zhen
- Applied and Environmental Microbiology, Vol. 78, Issue 13
Expression of abrB310 and sinR, and Effects of Decreased abrB310 Expression on the Transition from Acidogenesis to Solventogenesis, in Clostridium acetobutylicum ATCC 824
journal, April 2005
- Scotcher, M. C.; Rudolph, F. B.; Bennett, G. N.
- Applied and Environmental Microbiology, Vol. 71, Issue 4
A Life with Acetogens, Thermophiles, and Cellulolytic Anaerobes
journal, October 2009
- Ljungdahl, Lars G.
- Annual Review of Microbiology, Vol. 63, Issue 1
Development and Characterization of a Gene Expression Reporter System for Clostridium acetobutylicumATCC 824
journal, September 1999
- Tummala, Seshu B.; Welker, Neil E.; Papoutsakis, Eleftherios T.
- Applied and Environmental Microbiology, Vol. 65, Issue 9
Sporulation and Enterotoxin (CPE) Synthesis Are Controlled by the Sporulation-Specific Sigma Factors SigE and SigK in Clostridium perfringens
journal, February 2009
- Harry, K. H.; Zhou, R.; Kroos, L.
- Journal of Bacteriology, Vol. 191, Issue 8
Bet-hedging and epigenetic inheritance in bacterial cell development
journal, March 2008
- Veening, J. -W.; Stewart, E. J.; Berngruber, T. W.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 11
The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein.
journal, May 1989
- Strauch, M. A.; Spiegelman, G. B.; Perego, M.
- The EMBO Journal, Vol. 8, Issue 5
Bistability, Epigenetics, and Bet-Hedging in Bacteria
journal, October 2008
- Veening, Jan-Willem; Smits, Wiep Klaas; Kuipers, Oscar P.
- Annual Review of Microbiology, Vol. 62, Issue 1
Expression of kinA and kinB of Bacillus subtilis, Necessary for Sporulation Initiation, Is under Positive Stringent Transcription Control
journal, February 2013
- Tojo, S.; Hirooka, K.; Fujita, Y.
- Journal of Bacteriology, Vol. 195, Issue 8
Regulation of transcription of the Bacillus subtilis spoIIA locus.
journal, February 1989
- Wu, J. J.; Howard, M. G.; Piggot, P. J.
- Journal of Bacteriology, Vol. 171, Issue 2
Promoter specificity of sigma G-containing RNA polymerase from sporulating cells of Bacillus subtilis: identification of a group of forespore-specific promoters.
journal, May 1989
- Nicholson, W. L.; Sun, D. X.; Setlow, B.
- Journal of Bacteriology, Vol. 171, Issue 5
Deciding fate in adverse times: Sporulation and competence in Bacillus subtilis
journal, December 2009
- Schultz, Daniel; Wolynes, Peter G.; Jacob, Eshel Ben
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 50
Role of the Anti-Sigma Factor SpoIIAB in Regulation of G during Bacillus subtilis Sporulation
journal, June 2004
- Serrano, M.; Neves, A.; Soares, C. M.
- Journal of Bacteriology, Vol. 186, Issue 12
Control of developmental transcription factor sigma F by sporulation regulatory proteins SpoIIAA and SpoIIAB in Bacillus subtilis.
journal, December 1990
- Schmidt, R.; Margolis, P.; Duncan, L.
- Proceedings of the National Academy of Sciences, Vol. 87, Issue 23
Control of the initiation of sporulation in Bacillus subtilis by a phosphorelay
journal, January 1991
- Trach, K.; Burbulys, D.; Strauch, M.
- Research in Microbiology, Vol. 142, Issue 7-8
Bile Salts and Glycine as Cogerminants for Clostridium difficile Spores
journal, February 2008
- Sorg, J. A.; Sonenshein, A. L.
- Journal of Bacteriology, Vol. 190, Issue 7
Binding of the Bacillus subtilis spoIVCA product to the recombination sites of the element interrupting the sigma K-encoding gene.
journal, July 1992
- Popham, D. L.; Stragier, P.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 13
Regulation of the Phosphorelay and the Initiation of Sporulation in Bacillus Subtilis
journal, October 1993
- Hoch, J. A.
- Annual Review of Microbiology, Vol. 47, Issue 1
Genome Sequence and Comparative Analysis of the Solvent-Producing Bacterium Clostridium acetobutylicum
journal, August 2001
- Nolling, J.; Breton, G.; Omelchenko, M. V.
- Journal of Bacteriology, Vol. 183, Issue 16
Cell-cell signaling pathway activating a developmental transcription factor in Bacillus subtilis.
journal, February 1995
- Londono-Vallejo, J. A.; Stragier, P.
- Genes & Development, Vol. 9, Issue 4
The transcriptional program underlying the physiology of clostridial sporulation
journal, January 2008
- Jones, Shawn W.; Paredes, Carlos J.; Tracy, Bryan
- Genome Biology, Vol. 9, Issue 7
K of Clostridium acetobutylicum Is the First Known Sporulation-Specific Sigma Factor with Two Developmentally Separated Roles, One Early and One Late in Sporulation
journal, November 2013
- Al-Hinai, M. A.; Jones, S. W.; Papoutsakis, E. T.
- Journal of Bacteriology, Vol. 196, Issue 2
Switch protein alters specificity of RNA polymerase containing a compartment-specific sigma factor
journal, January 1989
- Kroos, L.; Kunkel, B.; Losick, R.
- Science, Vol. 243, Issue 4890
Sporulation Regulatory Protein SpoIIID from Bacillus subtilis Activates and Represses Transcription by Both Mother-cell-specific Forms of RNA Polymerase
journal, October 1994
- Halberg, Richard; Kroos, Lee
- Journal of Molecular Biology, Vol. 243, Issue 3
Timing of spoII gene expression relative to septum formation during sporulation of Bacillus subtilis.
journal, October 1989
- Gholamhoseinian, A.; Piggot, P. J.
- Journal of Bacteriology, Vol. 171, Issue 10
Colonisation ofClostridiumin the body is restricted to hypoxic and necrotic areas of tumours
journal, August 1998
- Lambin, P.; Theys, J.; Landuyt, W.
- Anaerobe, Vol. 4, Issue 4
A comparative genomic view of clostridial sporulation and physiology
journal, October 2005
- Paredes, Carlos J.; Alsaker, Keith V.; Papoutsakis, Eleftherios T.
- Nature Reviews Microbiology, Vol. 3, Issue 12
Bacillus sporulation gene spo0H codes for sigma 30 (sigma H).
journal, March 1988
- Dubnau, E.; Weir, J.; Nair, G.
- Journal of Bacteriology, Vol. 170, Issue 3
The relationship between sporulation and solvent production in clostridium acetobutylicum P262
journal, August 1984
- Long, Susan; Jones, David T.; Woods, David R.
- Biotechnology Letters, Vol. 6, Issue 8
Novel System for Efficient Isolation of Clostridium Double-Crossover Allelic Exchange Mutants Enabling Markerless Chromosomal Gene Deletions and DNA Integration
journal, September 2012
- Al-Hinai, Mohab A.; Fast, Alan G.; Papoutsakis, Eleftherios T.
- Applied and Environmental Microbiology, Vol. 78, Issue 22
RNA Polymerase and Alternative σ Factors
book, March 2005
- Dupuy, Bruno; Sonenshein, Abraham; Haraldsen, Jeralyn
- Handbook on Clostridia
Chromosomal rearrangement generating a composite gene for a developmental transcription factor
journal, January 1989
- Stragier, P.; Kunkel, B.; Kroos, L.
- Science, Vol. 243, Issue 4890
Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater
journal, January 2002
- Shimizu, T.; Ohtani, K.; Hirakawa, H.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 2
Chemotherapeutic tumour targeting using clostridial spores
journal, October 1995
- Minton, Nigel P.; Mauchline, Margaret L.; Lemmon, Marylin J.
- FEMS Microbiology Reviews, Vol. 17, Issue 3
Bacillus subtilis early sporulation genes kinA, spo0F, and spo0A are transcribed by the RNA polymerase containing sigma H.
journal, May 1992
- Predich, M.; Nair, G.; Smith, I.
- Journal of Bacteriology, Vol. 174, Issue 9
Evaluating the Involvement of Alternative Sigma Factors SigF and SigG in Clostridium perfringens Sporulation and Enterotoxin Synthesis
journal, July 2010
- Li, J.; McClane, B. A.
- Infection and Immunity, Vol. 78, Issue 10
Genome-Wide Analysis of Cell Type-Specific Gene Transcription during Spore Formation in Clostridium difficile
journal, October 2013
- Saujet, Laure; Pereira, Fátima C.; Serrano, Monica
- PLoS Genetics, Vol. 9, Issue 10
Bacterial sporulation: A question of commitment?
journal, January 1998
- Stephens, Craig
- Current Biology, Vol. 8, Issue 2
The ClosTron: A universal gene knock-out system for the genus Clostridium
journal, September 2007
- Heap, John T.; Pennington, Oliver J.; Cartman, Stephen T.
- Journal of Microbiological Methods, Vol. 70, Issue 3
MOLECULAR GENETICS OF SPORULATION IN BACILLUS SUBTILIS
journal, December 1996
- Stragier, Patrick; Losick, Richard
- Annual Review of Genetics, Vol. 30, Issue 1
Sporulation-specific sigma factor sigma 29 of Bacillus subtilis is synthesized from a precursor protein, P31.
journal, April 1987
- LaBell, T. L.; Trempy, J. E.; Haldenwang, W. G.
- Proceedings of the National Academy of Sciences, Vol. 84, Issue 7
Structurally diverse natural products that cause potassium leakage trigger multicellularity in Bacillus subtilis
journal, December 2008
- Lopez, D.; Fischbach, M. A.; Chu, F.
- Proceedings of the National Academy of Sciences, Vol. 106, Issue 1
Activation of spo0A transcription by sigma H is necessary for sporulation but not for competence in Bacillus subtilis.
journal, June 1994
- Siranosian, K. J.; Grossman, A. D.
- Journal of Bacteriology, Vol. 176, Issue 12
Prespore-specific gene expression in Bacillus subtilis is driven by sequestration of SpoIIE phosphatase to the prespore side of the asymmetric septum
journal, May 1998
- Wu, L. J.; Feucht, A.; Errington, J.
- Genes & Development, Vol. 12, Issue 9
Septation, dephosphorylation, and the activation of sigma F during sporulation in Bacillus subtilis
journal, May 1999
- King, N.; Dreesen, O.; Stragier, P.
- Genes & Development, Vol. 13, Issue 9
Hierarchical Evolution of the Bacterial Sporulation Network
journal, September 2010
- de Hoon, Michiel J. L.; Eichenberger, Patrick; Vitkup, Dennis
- Current Biology, Vol. 20, Issue 17
Delineation of AbrB-binding sites on the Bacillus subtilis spo0H, kinB, ftsAZ, and pbpE promoters and use of a derived homology to identify a previously unsuspected binding site in the bsuB1 methylase promote.
journal, December 1995
- Strauch, M. A.
- Journal of Bacteriology, Vol. 177, Issue 23
Regulation of Bacillus subtilis sigmaH (spo0H) and AbrB in response to changes in external pH.
journal, November 1997
- Cosby, W. M.; Zuber, P.
- Journal of Bacteriology, Vol. 179, Issue 21
The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression.
journal, August 1988
- Kunkel, B.; Sandman, K.; Panzer, S.
- Journal of Bacteriology, Vol. 170, Issue 8
Complete nucleotide sequence of a skin element excised by DNA rearrangement during sporulation in Bacillus subtilis
journal, February 1995
- Takemaru, K. -i.; Mizuno, M.; Sato, T.
- Microbiology, Vol. 141, Issue 2
Northern, Morphological, and Fermentation Analysis of spo0A Inactivation and Overexpression in Clostridium acetobutylicum ATCC 824
journal, July 2002
- Harris, L. M.; Welker, N. E.; Papoutsakis, E. T.
- Journal of Bacteriology, Vol. 184, Issue 13
Efficient sporulation in Clostridium difficile requires disruption of the σK gene: Gene disruption essential for sporulation
journal, April 2003
- Haraldsen, Jeralyn D.; Sonenshein, Abraham L.
- Molecular Microbiology, Vol. 48, Issue 3
Genetic evidence that RNA polymerase associated with sigma A factor uses a sporulation-specific promoter in Bacillus subtilis.
journal, December 1989
- Kenney, T. J.; York, K.; Youngman, P.
- Proceedings of the National Academy of Sciences, Vol. 86, Issue 23
The Forespore Line of Gene Expression in Bacillus subtilis
journal, April 2006
- Wang, Stephanie T.; Setlow, Barbara; Conlon, Erin M.
- Journal of Molecular Biology, Vol. 358, Issue 1
Regulation of spo0H, a gene coding for the Bacillus subtilis sigma H factor.
journal, January 1991
- Weir, J.; Predich, M.; Dubnau, E.
- Journal of Bacteriology, Vol. 173, Issue 2
Alternative Sigma Factors SigF, SigE, and SigG Are Essential for Sporulation in Clostridium botulinum ATCC 3502
journal, June 2014
- Kirk, David G.; Zhang, Zhen; Korkeala, Hannu
- Applied and Environmental Microbiology, Vol. 80, Issue 16
Expression of spoIIIJ in the Prespore Is Sufficient for Activation of G and for Sporulation in Bacillus subtilis
journal, July 2003
- Serrano, M.; Corte, L.; Opdyke, J.
- Journal of Bacteriology, Vol. 185, Issue 13
Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis.
journal, January 1993
- Errington, J.
- Microbiological Reviews, Vol. 57, Issue 1
Genome sequence of a proteolytic (Group I) Clostridium botulinum strain Hall A and comparative analysis of the clostridial genomes
journal, June 2007
- Sebaihia, M.; Peck, M. W.; Minton, N. P.
- Genome Research, Vol. 17, Issue 7
Transcriptional organization of the Clostridium acetobutylicum genome
journal, March 2004
- Paredes, C. J.
- Nucleic Acids Research, Vol. 32, Issue 6
Sporulation of Bacillus subtilis
journal, December 2004
- Piggot, Patrick J.; Hilbert, David W.
- Current Opinion in Microbiology, Vol. 7, Issue 6
Inactivation of E and G in Clostridium acetobutylicum Illuminates Their Roles in Clostridial-Cell-Form Biogenesis, Granulose Synthesis, Solventogenesis, and Spore Morphogenesis
journal, January 2011
- Tracy, B. P.; Jones, S. W.; Papoutsakis, E. T.
- Journal of Bacteriology, Vol. 193, Issue 6
Genetic Networks Controlling the Initiation of Sporulation and the Development of Genetic Competence in Bacillus subtilis
journal, December 1995
- Grossman, Alan D.
- Annual Review of Genetics, Vol. 29, Issue 1
The mechanism of cell differentiation in Bacillus subtilis
journal, May 2006
- Iber, Dagmar; Clarkson, Joanna; Yudkin, Michael D.
- Nature, Vol. 441, Issue 7091
Development and Application of Flow-Cytometric Techniques for Analyzing and Sorting Endospore-Forming Clostridia
journal, October 2008
- Tracy, B. P.; Gaida, S. M.; Papoutsakis, E. T.
- Applied and Environmental Microbiology, Vol. 74, Issue 24
SpoIIE governs the phosphorylation state of a protein regulating transcription factor sigma F during sporulation in Bacillus subtilis.
journal, April 1996
- Arigoni, F.; Duncan, L.; Alper, S.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 8
The sigma factors of Bacillus subtilis.
journal, January 1995
- Haldenwang, W. G.
- Microbiological reviews, Vol. 59, Issue 1
The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain.
journal, September 1997
- Cornillot, E.; Nair, R. V.; Papoutsakis, E. T.
- Journal of Bacteriology, Vol. 179, Issue 17
Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis.
journal, February 1989
- Sun, D. X.; Stragier, P.; Setlow, P.
- Genes & Development, Vol. 3, Issue 2
Analysis of a suppressor mutation ssb (kinC) of sur0B20 (spo0A) mutation in Bacillus subtilis reveals that kinC encodes a histidine protein kinase.
journal, January 1995
- Kobayashi, K.; Shoji, K.; Shimizu, T.
- Journal of Bacteriology, Vol. 177, Issue 1
Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis
journal, November 1988
- Perego, M.; Spiegelman, G. B.; Hoch, J. A.
- Molecular Microbiology, Vol. 2, Issue 6
rpoD operon promoter used by sigma H-RNA polymerase in Bacillus subtilis.
journal, April 1988
- Carter, H. L.; Wang, L. F.; Doi, R. H.
- Journal of Bacteriology, Vol. 170, Issue 4
Cloning, nucleotide sequencing, and expression of the Clostridium perfringens enterotoxin gene in Escherichia coli.
journal, January 1993
- Czeczulin, J. R.; Hanna, P. C.; McClane, B. A.
- Infection and Immunity, Vol. 61, Issue 8
The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene.
journal, April 1990
- Kunkel, B.; Losick, R.; Stragier, P.
- Genes & Development, Vol. 4, Issue 4
Features of Pro- K Important for Cleavage by SpoIVFB, an Intramembrane Metalloprotease
journal, April 2013
- Zhou, R.; Chen, K.; Xiang, X.
- Journal of Bacteriology, Vol. 195, Issue 12
Genetic regulation of morphogenesis in Bacillus subtilis: roles of sigma E and sigma F in prespore engulfment.
journal, May 1991
- Illing, N.; Errington, J.
- Journal of Bacteriology, Vol. 173, Issue 10
SpoIIE Is Necessary for Asymmetric Division, Sporulation, and Expression of F, E, and G but Does Not Control Solvent Production in Clostridium acetobutylicum ATCC 824
journal, July 2011
- Bi, C.; Jones, S. W.; Hess, D. R.
- Journal of Bacteriology, Vol. 193, Issue 19
Inactivation of F in Clostridium acetobutylicum ATCC 824 Blocks Sporulation Prior to Asymmetric Division and Abolishes E and G Protein Expression but Does Not Block Solvent Formation
journal, March 2011
- Jones, S. W.; Tracy, B. P.; Gaida, S. M.
- Journal of Bacteriology, Vol. 193, Issue 10
Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum
journal, November 2007
- Shao, Lijun; Hu, Shiyuan; Yang, Yi
- Cell Research, Vol. 17, Issue 11
The Biocide Chlorine Dioxide Stimulates Biofilm Formation in Bacillus subtilis by Activation of the Histidine Kinase KinC
journal, October 2010
- Shemesh, M.; Kolter, R.; Losick, R.
- Journal of Bacteriology, Vol. 192, Issue 24
Tandem genes encoding sigma-factors for consecutive steps of development in Bacillus subtilis.
journal, February 1989
- Karmazyn-Campelli, C.; Bonamy, C.; Savelli, B.
- Genes & Development, Vol. 3, Issue 2
Sporulation protein SpoIVFB from Bacillus subtilis enhances processing of the sigma factor precursor Pro-sigma K in the absence of other sporulation gene products.
journal, February 1995
- Lu, S.; Cutting, S.; Kroos, L.
- Journal of Bacteriology, Vol. 177, Issue 4
Identification of a gene, spoIIR, that links the activation of sigma E to the transcriptional activity of sigma F during sporulation in Bacillus subtilis.
journal, March 1995
- Karow, M. L.; Glaser, P.; Piggot, P. J.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 6
The multidrug-resistant human pathogen Clostridium difficile has a highly mobile, mosaic genome
journal, June 2006
- Sebaihia, Mohammed; Wren, Brendan W.; Mullany, Peter
- Nature Genetics, Vol. 38, Issue 7
A genomic update on clostridial phylogeny: Gram-negative spore formers and other misplaced clostridia: Genomics update
journal, July 2013
- Yutin, Natalya; Galperin, Michael Y.
- Environmental Microbiology
Gut Microbiota in Health and Disease
journal, July 2010
- Sekirov, Inna; Russell, Shannon L.; Antunes, L. Caetano M.
- Physiological Reviews, Vol. 90, Issue 3
The biosynthesis of granulose by Clostridium pasteurianum
journal, December 1974
- Robson, R. L.; Robson, R. M.; Morris, J. G.
- Biochemical Journal, Vol. 144, Issue 3
Activation of Cell-Specific Transcription by a Serine Phosphatase at the Site of Asymmetric Division
journal, October 1995
- Duncan, L.; Alper, S.; Arigoni, F.
- Science, Vol. 270, Issue 5236
Recent progress in Bacillus subtilis sporulation
journal, January 2012
- Higgins, Douglas; Dworkin, Jonathan
- FEMS Microbiology Reviews, Vol. 36, Issue 1
Direct Analysis of Genes Encoding 16S rRNA from Complex Communities Reveals Many Novel Molecular Species within the Human Gut
journal, November 1999
- Suau, Antonia; Bonnet, Régis; Sutren, Malène
- Applied and Environmental Microbiology, Vol. 65, Issue 11
Phosphorylation and functional analysis of the sporulation initiation factor Spo0A from Clostridium botulinum
journal, February 2006
- Worner, Kristina; Szurmant, Hendrik; Chiang, Christina
- Molecular Microbiology, Vol. 59, Issue 3
AbrB and Spo0E Control the Proper Timing of Sporulation in Bacillus subtilis
journal, April 2004
- Shafikhani, Sasha H.; Leighton, Terrance
- Current Microbiology, Vol. 48, Issue 4
Regulation of endospore formation in Bacillus subtilis
journal, November 2003
- Errington, Jeff
- Nature Reviews Microbiology, Vol. 1, Issue 2
The Spore Differentiation Pathway in the Enteric Pathogen Clostridium difficile
journal, October 2013
- Pereira, Fátima C.; Saujet, Laure; Tomé, Ana R.
- PLoS Genetics, Vol. 9, Issue 10
Global Analysis of the Sporulation Pathway of Clostridium difficile
journal, August 2013
- Fimlaid, Kelly A.; Bond, Jeffrey P.; Schutz, Kristin C.
- PLoS Genetics, Vol. 9, Issue 8
Compartmentalized expression of a gene under the control of sporulation transcription factor sigma E in Bacillus subtilis.
journal, November 1991
- Driks, A.; Losick, R.
- Proceedings of the National Academy of Sciences, Vol. 88, Issue 22
The SpoIIE phosphatase, the sporulation septum and the establishment of forespore-specific transcription in Bacillus subtilis: a reassessment
journal, March 1999
- Arigoni, Fabrizio; Guerout-Fleury, Anne-Marie; Barak, Imrich
- Molecular Microbiology, Vol. 31, Issue 5
Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis.
journal, November 1989
- Perego, M.; Cole, S. P.; Burbulys, D.
- Journal of Bacteriology, Vol. 171, Issue 11
The Firmicutes/Bacteroidetes ratio of the human microbiota changes with age
journal, January 2009
- Mariat, D.; Firmesse, O.; Levenez, F.
- BMC Microbiology, Vol. 9, Issue 1
The Spo0A regulon of Bacillus subtilis: The Spo0A regulon
journal, November 2003
- Molle, Virginie; Fujita, Masaya; Jensen, Shane T.
- Molecular Microbiology, Vol. 50, Issue 5
A forespore checkpoint for mother cell gene expression during development in B. subtilis
journal, July 1990
- Cutting, Simon; Oke, Valerie; Driks, Adam
- Cell, Vol. 62, Issue 2
Solvent Production and Morphological Changes in Clostridium acetobutylicum
journal, January 1982
- Jones, D. T.; van der Westhuizen, A.; Long, S.
- Applied and Environmental Microbiology, Vol. 43, Issue 6
Binding of Spo0A stimulates spoIIG promoter activity in Bacillus subtilis.
journal, March 1992
- Satola, S. W.; Baldus, J. M.; Moran, C. P.
- Journal of Bacteriology, Vol. 174, Issue 5
The sigma factors of Bacillus subtilis
journal, March 1995
- Haldenwang, W. G.
- Microbiological Reviews, Vol. 59, Issue 1
Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis
journal, March 1993
- Errington, J.
- Microbiological Reviews, Vol. 57, Issue 1
The genome sequence of Clostridium tetani, the causative agent of tetanus disease
text, January 2003
- Brüggemann, Holger; Baumer, Sebastian; Fricke, Wolfgang Florian
- Universität Regensburg
Works referencing / citing this record:
Capturing One of the Human Gut Microbiome’s Most Wanted: Reconstructing the Genome of a Novel Butyrate-Producing, Clostridial Scavenger from Metagenomic Sequence Data
journal, May 2016
- Jeraldo, Patricio; Hernandez, Alvaro; Nielsen, Henrik B.
- Frontiers in Microbiology, Vol. 7
RNAseq-based transcriptome assembly of Clostridium acetobutylicum for functional genome annotation and discovery
journal, October 2018
- Ralston, Matthew T.; Papoutsakis, Eleftherios T.
- AIChE Journal, Vol. 64, Issue 12
Microbial solvent formation revisited by comparative genome analysis
journal, March 2017
- Poehlein, Anja; Solano, José David Montoya; Flitsch, Stefanie K.
- Biotechnology for Biofuels, Vol. 10, Issue 1
Effect of fermentation system on the physicochemical and microbial community dynamics during enset ( Ensete ventricosum ) fermentation
journal, January 2019
- Andeta, A. F.; Vandeweyer, D.; Teffera, E. F.
- Journal of Applied Microbiology, Vol. 126, Issue 3
Whole-genome sequence of an evolved Clostridium pasteurianum strain reveals Spo0A deficiency responsible for increased butanol production and superior growth
journal, December 2015
- Sandoval, Nicholas R.; Venkataramanan, Keerthi P.; Groth, Theodore S.
- Biotechnology for Biofuels, Vol. 8, Issue 1
Genomics of Clostridium taeniosporum, an organism which forms endospores with ribbon-like appendages
journal, January 2018
- Cambridge, Joshua M.; Blinkova, Alexandra L.; Salvador Rocha, Erick I.
- PLOS ONE, Vol. 13, Issue 1
A Strongly Fluorescing Anaerobic Reporter and Protein-Tagging System for Clostridium Organisms Based on the Fluorescence-Activating and Absorption-Shifting Tag Protein (FAST)
journal, May 2019
- Streett, Hannah E.; Kalis, Katie M.; Papoutsakis, Eleftherios T.
- Applied and Environmental Microbiology, Vol. 85, Issue 14
The industrial anaerobe Clostridium acetobutylicum uses polyketides to regulate cellular differentiation
journal, November 2017
- Herman, Nicolaus A.; Kim, Seong Jong; Li, Jeffrey S.
- Nature Communications, Vol. 8, Issue 1
The characteristics of Clostridium difficile ST81, a new PCR ribotype of toxin A- B+ strain with high-level fluoroquinolones resistance and higher sporulation ability than ST37/PCR ribotype 017
journal, July 2018
- Wang, Baoya; Peng, Wenwen; Zhang, Pingping
- FEMS Microbiology Letters, Vol. 365, Issue 17
Differential requirements for conserved peptidoglycan remodeling enzymes during Clostridioides difficile spore formation: Regulation of Clostridium difficile Engulfment
journal, October 2018
- Ribis, John W.; Fimlaid, Kelly A.; Shen, Aimee
- Molecular Microbiology, Vol. 110, Issue 3
Transcription profiling of butanol producer Clostridium beijerinckii NRRL B-598 using RNA-Seq
journal, May 2018
- Sedlar, Karel; Koscova, Pavlina; Vasylkivska, Maryna
- BMC Genomics, Vol. 19, Issue 1
Clostridium butyricum maximizes growth while minimizing enzyme usage and ATP production: metabolic flux distribution of a strain cultured in glycerol
journal, June 2017
- Serrano-Bermúdez, Luis Miguel; González Barrios, Andrés Fernando; Maranas, Costas D.
- BMC Systems Biology, Vol. 11, Issue 1
Optimization of alkaline protease production by rational deletion of sporulation related genes in Bacillus licheniformis
journal, July 2019
- Zhou, Cuixia; Zhou, Huiying; Zhang, Huitu
- Microbial Cell Factories, Vol. 18, Issue 1
Flexibility and constraint: Evolutionary remodeling of the sporulation initiation pathway in Firmicutes
journal, September 2018
- Davidson, Philip; Eutsey, Rory; Redler, Brendan
- PLOS Genetics, Vol. 14, Issue 9
Characterization of Clostridium novyi isolated from a sow in a sudden death case in Korea
posted_content, May 2020
- Kim, Won-II; Jeong, Chang-Gi; Seo, Byoung-Joo
- Research Square [preprints]
Linking Microbial Community Structure and Function During the Acidified Anaerobic Digestion of Grass
journal, March 2018
- Joyce, Aoife; Ijaz, Umer Z.; Nzeteu, Corine
- Frontiers in Microbiology, Vol. 9
Comparison of expression of key sporulation, solventogenic and acetogenic genes in C. beijerinckii NRRL B-598 and its mutant strain overexpressing spo0A
journal, October 2017
- Kolek, J.; Diallo, M.; Vasylkivska, M.
- Applied Microbiology and Biotechnology, Vol. 101, Issue 22
Acidogenesis, solventogenesis, metabolic stress response and life cycle changes in Clostridium beijerinckii NRRL B-598 at the transcriptomic level
journal, February 2019
- Patakova, Petra; Branska, Barbora; Sedlar, Karel
- Scientific Reports, Vol. 9, Issue 1
Comparative genomics identifies potential virulence factors in Clostridium tertium and C. paraputrificum
journal, January 2019
- Muñoz, Marina; Restrepo-Montoya, Daniel; Kumar, Nitin
- Virulence, Vol. 10, Issue 1
System-level modeling of acetone–butanol–ethanol fermentation
journal, March 2016
- Liao, Chen; Seo, Seung-Oh; Lu, Ting
- FEMS Microbiology Letters, Vol. 363, Issue 9
Flexibility and constraint: Evolutionary remodeling of the sporulation initiation pathway in Firmicutes
text, January 2018
- Davidson, Phillip; Eutsey, Rory; Redler, Brendan
- Carnegie Mellon University
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posted_content, May 2020
- Jeong, Chang-Gi; Seo, Byoung-Joo; Nazki, Salik
- Research Square [preprints]
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text, January 2018
- Davidson, Phillip; Eutsey, Rory; Redler, Brendan
- Carnegie Mellon University