Sphingobium lignivorans sp. nov., isolated from river sediment downstream of a paper mill
Abstract
Here, a bacterial isolate, B1D3AT, was isolated from river sediment collected from the Hiwassee River near Calhoun, TN, by enrichment culturing with a model 5–5' lignin dimer, dehydrodivanillate, as its sole carbon source. B1D3AT was also shown to utilize several model lignin-derived monomers and dimers as sole carbon sources in a variety of minimal media. Cells were Gram-stain-negative, aerobic, motile, rod-shaped and formed yellow/cream-coloured colonies on rich agar. Optimal growth occurred at 30°C, pH 7–8, and in the absence of NaCl. The major fatty acids of B1D3AT were C18:1 ω7c and C17:1ω6c. The predominant hydroxy fatty acids were C14: 0 2-OH and C15:0 2-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethanolamine and sphingoglycolipid. B1D3AT contained spermidine as the only major polyamine. The major isoprenoid quinone was Q-10 with minor amounts of Q-9 and Q-11. The genomic DNA G+C content of B1D3AT was 65.6mol%. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 49 core, universal genes defined by Clusters of Orthologous Groups gene families indicated that B1D3AT was a member of the genus Sphingobium. B1D3AT was most closely related to Sphingobium sp. SYK-6, with a 100% 16S rRNA gene sequence similarity.more »
- Authors:
-
[1];
[2];
[2];
[2]
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Center for Bioenergy Innovation (CBI)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1928956
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- International Journal of Systematic and Evolutionary Microbiology
- Additional Journal Information:
- Journal Volume: 73; Journal Issue: 2; Journal ID: ISSN 1466-5026
- Publisher:
- International Union of Microbiological Societies
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; lignin degradation; Sphingobium; SYK-6
Citation Formats
Allemann, Marco N., Presley, Gerald N., Elkins, James G., and Michener, Joshua K. Sphingobium lignivorans sp. nov., isolated from river sediment downstream of a paper mill. United States: N. p., 2023.
Web. doi:10.1099/ijsem.0.005704.
Allemann, Marco N., Presley, Gerald N., Elkins, James G., & Michener, Joshua K. Sphingobium lignivorans sp. nov., isolated from river sediment downstream of a paper mill. United States. https://doi.org/10.1099/ijsem.0.005704
Allemann, Marco N., Presley, Gerald N., Elkins, James G., and Michener, Joshua K. Wed .
"Sphingobium lignivorans sp. nov., isolated from river sediment downstream of a paper mill". United States. https://doi.org/10.1099/ijsem.0.005704. https://www.osti.gov/servlets/purl/1928956.
@article{osti_1928956,
title = {Sphingobium lignivorans sp. nov., isolated from river sediment downstream of a paper mill},
author = {Allemann, Marco N. and Presley, Gerald N. and Elkins, James G. and Michener, Joshua K.},
abstractNote = {Here, a bacterial isolate, B1D3AT, was isolated from river sediment collected from the Hiwassee River near Calhoun, TN, by enrichment culturing with a model 5–5' lignin dimer, dehydrodivanillate, as its sole carbon source. B1D3AT was also shown to utilize several model lignin-derived monomers and dimers as sole carbon sources in a variety of minimal media. Cells were Gram-stain-negative, aerobic, motile, rod-shaped and formed yellow/cream-coloured colonies on rich agar. Optimal growth occurred at 30°C, pH 7–8, and in the absence of NaCl. The major fatty acids of B1D3AT were C18:1 ω7c and C17:1ω6c. The predominant hydroxy fatty acids were C14: 0 2-OH and C15:0 2-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidyldimethylethanolamine and sphingoglycolipid. B1D3AT contained spermidine as the only major polyamine. The major isoprenoid quinone was Q-10 with minor amounts of Q-9 and Q-11. The genomic DNA G+C content of B1D3AT was 65.6mol%. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 49 core, universal genes defined by Clusters of Orthologous Groups gene families indicated that B1D3AT was a member of the genus Sphingobium. B1D3AT was most closely related to Sphingobium sp. SYK-6, with a 100% 16S rRNA gene sequence similarity. B1D3AT showed 78.1–89.9%average nucleotide identity and 19.5–22.2% digital DNA–DNA hybridization identity with other type strains from the genus Sphingobium. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain B1D3AT should be classified as representing a novel species of the genus Sphingobium, for which the name Sphingobium lignivorans sp. nov. is proposed. The type strain is strain B1D3AT (ATCC TSD-279T=DSM 111877T).},
doi = {10.1099/ijsem.0.005704},
journal = {International Journal of Systematic and Evolutionary Microbiology},
number = 2,
volume = 73,
place = {United States},
year = {Wed Feb 15 00:00:00 EST 2023},
month = {Wed Feb 15 00:00:00 EST 2023}
}
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