Turicibacter bilis sp. nov., a novel bacterium isolated from the chicken eggshell and swine ileum

Maki, Joel J.; Looft, Torey

doi:10.1099/ijsem.0.005153

Title: Turicibacter bilis sp. nov., a novel bacterium isolated from the chicken eggshell and swine ileum

Journal Article · 26 January 2022 · International Journal of Systematic and Evolutionary Microbiology

DOI: https://doi.org/10.1099/ijsem.0.005153 · OSTI ID:1905047

^[1]; Looft, Torey ^[2]

US Dept. of Agriculture (USDA), Ames, IA (United States). Agricultural Research Service (ARS); Iowa State Univ., Ames, IA (United States); Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN (United States). ARS Research Participation Program; OSTI
US Dept. of Agriculture (USDA), Ames, IA (United States). Agricultural Research Service (ARS)

Three novel, anaerobic, Gram-positive bacteria were isolated from the eggshell of two separate white leghorn chicken flocks and the ileum of a healthy pig, and designated MMM721^T, ISU324 and PIG517 respectively. Cells were pleomorphic and capable of forming long chains of rods or coccoid clusters. Phylogenetic analysis of the 16S rRNA gene sequences identified these strains to be within the genus Turicibacter, of which only one species, Turicibacter sanguinis, has been formally described. However, whole genome sequencing of novel isolates returned a digital DNA–DNA hybridization value of 22.5% and average nucleotide identity (ANI) values of 76.4% (ANIb) and 86.0% (ANIm), indicating divergence between the type strain MMM721^T and T. sanguinis, suggesting the strains represented a novel species. The major fatty acid methyl esters of strain MMM721^T were C_16:0, C_18:1 ω7c and C_18:0. The strains mainly produced the volatile fatty acid lactate, along with smaller amounts of acetate and butyrate. Together, these data indicate that MMM721^T, along with ISU324 and PIG517, represent a novel species within the genus Turicibacter. We propose the name Turicibacter bilis sp. nov. for the species. The type strain is MMM721^T (=ATCC TSD-238^T=CCUG 74757^T).

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Research Organization:: Oak Ridge Inst. for Science and Education (ORISE), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0014664

OSTI ID:: 1905047

Journal Information:: International Journal of Systematic and Evolutionary Microbiology, Journal Name: International Journal of Systematic and Evolutionary Microbiology Journal Issue: 1 Vol. 72; ISSN 1466-5026

Publisher:: International Union of Microbiological SocietiesCopyright Statement

Country of Publication:: United States

Language:: English

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