Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum)
Abstract
Switchgrass is a promising biofuel feedstock due to its high biomass production and low agronomic input requirements. Because the bulk of switchgrass biomass used for biofuel production is lignocellulosic secondary walls, studies on secondary wall biosynthesis and its transcriptional regulation are imperative for designing strategies for genetic improvement of biomass production in switchgrass. Here, we report the identification and functional characterization of a group of switchgrass transcription factors, including several NACs (PvSWNs) and a MYB (PvMYB46A), for their involvement in regulating secondary wall biosynthesis. PvSWNs and PvMYB46A were found to be highly expressed in stems and their expression was closely associated with sclerenchyma cells. Overexpression of PvSWNs and PvMYB46A in Arabidopsis was shown to result in activation of the biosynthetic genes for cellulose, xylan and lignin and ectopic deposition of secondary walls in normally parenchymatous cells. Transactivation and complementation studies demonstrated that PvSWNs were able to activate the SNBE-driven GUS reporter gene and effectively rescue the secondary wall defects in the Arabidopsis snd1 nst1 double mutant, indicating that they are functional orthologs of Arabidopsis SWNs. Furthermore, we showed that PvMYB46A could activate the SMRE-driven GUS reporter gene and complement the Arabidopsis myb46 myb83 double mutant, suggesting that it is amore »
- Authors:
-
- University of Georgia, Athens, GA (United States)
- Publication Date:
- Research Org.:
- Univ. of Georgia, Athens, GA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division (CSGB); National Science Foundation (NSF)
- OSTI Identifier:
- 1904686
- Grant/Contract Number:
- FG02-03ER15415; ISO-1051900
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 10; Journal Issue: 8; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Arabidopsis thaliana; lignin; biosynthesis; cellulose; cell staining; leaves; reporter genes; transcription factors
Citation Formats
Zhong, Ruiqin, Yuan, Youxi, Spiekerman, John J., Guley, Joshua T., Egbosiuba, Janefrances C., and Ye, Zheng-Hua. Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum). United States: N. p., 2015.
Web. doi:10.1371/journal.pone.0134611.
Zhong, Ruiqin, Yuan, Youxi, Spiekerman, John J., Guley, Joshua T., Egbosiuba, Janefrances C., & Ye, Zheng-Hua. Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum). United States. https://doi.org/10.1371/journal.pone.0134611
Zhong, Ruiqin, Yuan, Youxi, Spiekerman, John J., Guley, Joshua T., Egbosiuba, Janefrances C., and Ye, Zheng-Hua. Thu .
"Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum)". United States. https://doi.org/10.1371/journal.pone.0134611. https://www.osti.gov/servlets/purl/1904686.
@article{osti_1904686,
title = {Functional Characterization of NAC and MYB Transcription Factors Involved in Regulation of Biomass Production in Switchgrass (Panicum virgatum)},
author = {Zhong, Ruiqin and Yuan, Youxi and Spiekerman, John J. and Guley, Joshua T. and Egbosiuba, Janefrances C. and Ye, Zheng-Hua},
abstractNote = {Switchgrass is a promising biofuel feedstock due to its high biomass production and low agronomic input requirements. Because the bulk of switchgrass biomass used for biofuel production is lignocellulosic secondary walls, studies on secondary wall biosynthesis and its transcriptional regulation are imperative for designing strategies for genetic improvement of biomass production in switchgrass. Here, we report the identification and functional characterization of a group of switchgrass transcription factors, including several NACs (PvSWNs) and a MYB (PvMYB46A), for their involvement in regulating secondary wall biosynthesis. PvSWNs and PvMYB46A were found to be highly expressed in stems and their expression was closely associated with sclerenchyma cells. Overexpression of PvSWNs and PvMYB46A in Arabidopsis was shown to result in activation of the biosynthetic genes for cellulose, xylan and lignin and ectopic deposition of secondary walls in normally parenchymatous cells. Transactivation and complementation studies demonstrated that PvSWNs were able to activate the SNBE-driven GUS reporter gene and effectively rescue the secondary wall defects in the Arabidopsis snd1 nst1 double mutant, indicating that they are functional orthologs of Arabidopsis SWNs. Furthermore, we showed that PvMYB46A could activate the SMRE-driven GUS reporter gene and complement the Arabidopsis myb46 myb83 double mutant, suggesting that it is a functional ortholog of Arabidopsis MYB46/MYB83. Together, these results indicate that PvSWNs and PvMYB46A are transcriptional switches involved in regulating secondary wall biosynthesis, which provides molecular tools for genetic manipulation of biomass production in switchgrass.},
doi = {10.1371/journal.pone.0134611},
journal = {PLoS ONE},
number = 8,
volume = 10,
place = {United States},
year = {Thu Aug 06 00:00:00 EDT 2015},
month = {Thu Aug 06 00:00:00 EDT 2015}
}
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The NAC Transcription Factors NST1 and NST2 of Arabidopsis Regulate Secondary Wall Thickenings and Are Required for Anther Dehiscence
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SND1, a NAC Domain Transcription Factor, Is a Key Regulator of Secondary Wall Synthesis in Fibers of Arabidopsis
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The MYB46 Transcription Factor Is a Direct Target of SND1 and Regulates Secondary Wall Biosynthesis in Arabidopsis
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journal, December 1997
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Silencing of 4-coumarate:coenzyme A ligase in switchgrass leads to reduced lignin content and improved fermentable sugar yields for biofuel production
journal, July 2011
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- New Phytologist, Vol. 192, Issue 3
Functional characterization of the switchgrass (Panicum virgatum) R2R3-MYB transcription factor PvMYB4 for improvement of lignocellulosic feedstocks
journal, October 2011
- Shen, Hui; He, Xianzhi; Poovaiah, Charleson R.
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Comparative genomic analysis of the R2R3 MYB secondary cell wall regulators of Arabidopsis, poplar, rice, maize, and switchgrass
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- BMC Plant Biology, Vol. 14, Issue 1
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The Use of an Optical Brightener in the Study of Plant Structure
journal, January 1975
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Engineering the Oryza sativa cell wall with rice NAC transcription factors regulating secondary wall formation
journal, January 2013
- Yoshida, Kouki; Sakamoto, Shingo; Kawai, Tetsushi
- Frontiers in Plant Science, Vol. 4
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