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Title: Xylem vessel‐specific SND5 and its homologs regulate secondary wall biosynthesis through activating secondary wall NAC binding elements

Journal Article · · New Phytologist
DOI: https://doi.org/10.1111/nph.17425 · OSTI ID:1797599
 [1];  [2];  [1]; ORCiD logo [1]
  1. Department of Plant Biology University of Georgia Athens GA 30602 USA
  2. Department of Plant Biology University of Georgia Athens GA 30602 USA, Department of Plant &, Environmental New Resources College of Life Sciences Kyung Hee University Yongin 17104 Republic of Korea
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Summary Secondary cell wall biosynthesis has been shown to be regulated by a suite of transcription factors. Here, we identified a new xylem vessel‐specific NAC domain transcription factor, secondary wall‐associated NAC domain protein5 (SND5), in Arabidopsis thaliana and studied its role in regulating secondary wall biosynthesis. We showed that the expression of SND5 and its close homolog, SND4/ANAC075 , was specifically associated with secondary wall‐containing cells and dominant repression of their functions severely reduced secondary wall thickening in these cells. Overexpression of SND4/5 as well as their homologs SND2/3 fused with the activation domain of the viral protein VP16 led to ectopic secondary wall deposition in cells that are normally parenchymatous. SND2/3/4/5 regulated the expression of the same downstream target genes as do the secondary wall NAC master switches (SWNs) by binding to and activating the secondary wall NAC binding elements (SNBEs). Furthermore, we demonstrated that the poplar ( Populus trichocarpa ) orthologs of SND2/3/4/5 also activated SNBEs and regulated secondary wall biosynthesis during wood formation. Together, these findings indicate that SND2/3/4/5 and their poplar orthologs regulate the expression of secondary wall‐associated genes through activating SNBEs and they are positioned at an upper level in the SWN‐mediated transcriptional network.

Sponsoring Organization:
USDOE
Grant/Contract Number:
FG02-03ER15415
OSTI ID:
1797599
Journal Information:
New Phytologist, Journal Name: New Phytologist Journal Issue: 4 Vol. 231; ISSN 0028-646X
Publisher:
Wiley-BlackwellCopyright Statement
Country of Publication:
United Kingdom
Language:
English

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