Biomass formation and sugar release efficiency of Populus modified by altered expression of a NAC transcription factor
Abstract
Woody biomass is an important feedstock for biofuel production. Manipulation of wood properties that enable efficient conversion of biomass to biofuel reduces cost of biofuel production. Wood cell wall composition is regulated at several levels that involve expression of transcription factors such as wood-/secondary cell wall-associated NAC domains (WND or SND). In Arabidopsis thaliana, SND1 regulates cell wall composition through activation of its down-stream targets such as MYBs. The functional aspects of SND1 homologs in the woody Populus have been studied through transgenic manipulation. In this study, we investigated the role of PdWND1B, Populus SND1 sequence ortholog, in wood formation using transgenic manipulation through over-expression or silencing under the control of a vascular-specific 4-coumarate-CoA ligase (4CL) promoter. As compared with control plants, PdWND1B-RNAi plants were shorter in height, with significantly reduced stem diameter and dry biomass, whereas there were no significant differences in growth and productivity of PdWND1B over-expression plants. Conversely, PdWND1B over-expression lines showed a significant reduction in cellulose and increase in lignin content, whereas there was no significant impact on lignin content of downregulated lines. Stem carbohydrate composition analysis revealed a decrease in glucose, mannose, arabinose, and galactose, but an increase in xylose in the over-expression lines. Transcriptomemore »
- Authors:
-
[1];
[1];
[3];
[1];
[1];
[1];
[1]
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- ArborGen, Inc., Ridgeville, SC (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1883817
- Alternate Identifier(s):
- OSTI ID: 1884806
- Report Number(s):
- NREL/JA-2700-83855
Journal ID: ISSN 2475-4455
- Grant/Contract Number:
- AC05-00OR22725; AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Plant Direct
- Additional Journal Information:
- Journal Volume: 6; Journal Issue: 8; Journal ID: ISSN 2475-4455
- Publisher:
- Wiley and American Society of Plant Biologists and Society for Experimental Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; biomass; cell wall; cellulose; lignin; MYB; NAC transcription factor; SND; sugar release; WND1B
Citation Formats
Payyavula, Raja S., Badmi, Raghuram, Jawdy, Sara S., Rodriguez, Jr., Miguel, Gunter, Lee E., Sykes, Robert W., Winkeler, Kimberly A., Collins, Cassandra M., Rottmann, William H., Chen, Jin‐Gui, Yang, Xiaohan, Tuskan, Gerald A., and Kalluri, Udaya C. Biomass formation and sugar release efficiency of Populus modified by altered expression of a NAC transcription factor. United States: N. p., 2022.
Web. doi:10.1002/pld3.419.
Payyavula, Raja S., Badmi, Raghuram, Jawdy, Sara S., Rodriguez, Jr., Miguel, Gunter, Lee E., Sykes, Robert W., Winkeler, Kimberly A., Collins, Cassandra M., Rottmann, William H., Chen, Jin‐Gui, Yang, Xiaohan, Tuskan, Gerald A., & Kalluri, Udaya C. Biomass formation and sugar release efficiency of Populus modified by altered expression of a NAC transcription factor. United States. https://doi.org/10.1002/pld3.419
Payyavula, Raja S., Badmi, Raghuram, Jawdy, Sara S., Rodriguez, Jr., Miguel, Gunter, Lee E., Sykes, Robert W., Winkeler, Kimberly A., Collins, Cassandra M., Rottmann, William H., Chen, Jin‐Gui, Yang, Xiaohan, Tuskan, Gerald A., and Kalluri, Udaya C. Fri .
"Biomass formation and sugar release efficiency of Populus modified by altered expression of a NAC transcription factor". United States. https://doi.org/10.1002/pld3.419. https://www.osti.gov/servlets/purl/1883817.
@article{osti_1883817,
title = {Biomass formation and sugar release efficiency of Populus modified by altered expression of a NAC transcription factor},
author = {Payyavula, Raja S. and Badmi, Raghuram and Jawdy, Sara S. and Rodriguez, Jr., Miguel and Gunter, Lee E. and Sykes, Robert W. and Winkeler, Kimberly A. and Collins, Cassandra M. and Rottmann, William H. and Chen, Jin‐Gui and Yang, Xiaohan and Tuskan, Gerald A. and Kalluri, Udaya C.},
abstractNote = {Woody biomass is an important feedstock for biofuel production. Manipulation of wood properties that enable efficient conversion of biomass to biofuel reduces cost of biofuel production. Wood cell wall composition is regulated at several levels that involve expression of transcription factors such as wood-/secondary cell wall-associated NAC domains (WND or SND). In Arabidopsis thaliana, SND1 regulates cell wall composition through activation of its down-stream targets such as MYBs. The functional aspects of SND1 homologs in the woody Populus have been studied through transgenic manipulation. In this study, we investigated the role of PdWND1B, Populus SND1 sequence ortholog, in wood formation using transgenic manipulation through over-expression or silencing under the control of a vascular-specific 4-coumarate-CoA ligase (4CL) promoter. As compared with control plants, PdWND1B-RNAi plants were shorter in height, with significantly reduced stem diameter and dry biomass, whereas there were no significant differences in growth and productivity of PdWND1B over-expression plants. Conversely, PdWND1B over-expression lines showed a significant reduction in cellulose and increase in lignin content, whereas there was no significant impact on lignin content of downregulated lines. Stem carbohydrate composition analysis revealed a decrease in glucose, mannose, arabinose, and galactose, but an increase in xylose in the over-expression lines. Transcriptome analysis revealed upregulation of several downstream transcription factors and secondary cell wall related structural genes in the PdWND1B over-expression lines, partly explaining the observed phenotypic changes in cell wall chemistry. Relative to the control, glucose release efficiency and ethanol production from stem biomass was significantly reduced in over-expression lines. Our results show that PdWND1B is an important factor determining biomass productivity, cell wall chemistry and its conversion to biofuels in Populus.},
doi = {10.1002/pld3.419},
journal = {Plant Direct},
number = 8,
volume = 6,
place = {United States},
year = {Fri Aug 12 00:00:00 EDT 2022},
month = {Fri Aug 12 00:00:00 EDT 2022}
}
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