Title: A mechanistic study of cellulase adsorption onto lignin

Abstract

To explore the effect of lignin composition on cellulase adsorption, here dehydrogenation polymers (DHPs) were prepared from p-glucocoumaryl alcohol/coniferin/syringin, giving rise to H-DHP, G-DHP, and S-DHP, respectively. The structures of DHPs were thoroughly characterized and compared by GPC and NMR techniques, and the Langmuir isotherm protocol was applied to determine the cellulase adsorption behaviors of these different types of DHPs. The adsorption study indicated that the binding strength between the DHPs and cellulase varied in the following order: G-DHP > H-DHP > S-DHP. The inhibition of different types of DHPs on enzymatic hydrolysis of cellulose was in the same order as the cellulase adsorption, indicating that non-productive adsorption was the main way to influence cellulase. The correlation analysis results showed a positive association between the phenolic hydroxyl group content in DHPs and their maximum adsorption capacity toward enzymes. A negative correlation between the PDI and binding strength was also observed. It was also found that the adsorbed cellulase could be desorbed and retained normal enzyme activity, and so it was presumed that DHPs and cellulase were mainly linked by physisorption such as hydrogen bonding. This study clearly showed that the composition of lignin had a great impact on cellulase, andmore » that G-type lignin exhibited the most detrimental effect. The results could provide useful information on the mechanism of cellulase adsorption onto lignin using DHPs as lignin model compounds.« less

Authors:

Yao, Lan  ^[1];

• Search DOE PAGES for author "Yao, Lan"
• Search DOE PAGES for ORCID "0000-0003-3749-0210"

Yang, Haitao ^[2]; Yoo, Chang Geun  ^[3];

• Search DOE PAGES for author "Yoo, Chang Geun"
• Search DOE PAGES for ORCID "0000-0002-6179-2414"

Chen, Congxin ^[4]; Meng, Xianzhi  ^[5];

• Search DOE PAGES for author "Meng, Xianzhi"
• Search DOE PAGES for ORCID "0000-0003-4303-3403"

Dai, Jun ^[2]; Yang, Chunlei ^[6]; Yu, Jun ^[6]; Ragauskas, Arthur J.  ^[7];

• Search DOE PAGES for author "Ragauskas, Arthur J."
• Search DOE PAGES for ORCID "0000-0002-3536-554X"

Chen, Xiong ^[2]

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+ Show Author Affiliations

1. Hubei Univ. of Technology, Wuhan (China); Univ. of Tennessee, Knoxville, TN (United States)
2. Hubei Univ. of Technology, Wuhan (China)
3. State Univ. of New York (SUNY), Syracuse, NY (United States). College of Environmental Science and Forestry
4. China Tobacco Hubei Industrial Cigarette Materials LLC, Wuhan (China)
5. Univ. of Tennessee, Knoxville, TN (United States)
6. Tobacco Research Inst. of Hubei Province, Wuhan (China)
7. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Publication Date:

Wed Oct 28 00:00:00 EDT 2020

Research Org.:

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:

USDOE Office of Science (SC); National Natural Science Foundation of China (NSFC); Chinese Scholarship Council; Ministry of Education of China; Hubei Provincial Department of Education; Foundation of Hubei; Natural Science Foundation of Hubei Provincial Department of Education

OSTI Identifier:

1706228

Grant/Contract Number:  

AC05-00OR22725; 21978074; 31871789; 2011842330; 201508420257; KF201611; KF201719; D20161402; 201611B01; 201806A02; B2016046

Resource Type:

Accepted Manuscript

Journal Name:

Green Chemistry

Additional Journal Information:

Journal Volume: 23; Journal Issue: 1; Journal ID: ISSN 1463-9262

Publisher:

Royal Society of Chemistry

Country of Publication:

United States

Language:

English

Subject:

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; dehydrogenation polymer; cellulase; non-productive adsorption; enzymatic hydrolysis; desorption