Physico-chemical properties of lignin fractions from acid pretreated corn stover and their effects on enzymatic hydrolysis of microcrystalline cellulose
Abstract
Lignin has been shown to be a recalcitrance factor in many biomass conversion studies. To better understand the effects of lignin on cellulose conversion, different lignin fractions were extracted from the same dilute acid pretreated corn stover by three sequential isolation methods, namely ethanol extraction, dioxane extraction, and enzyme purification. The physicochemical properties of each lignin fraction including molecular weight distribution, surface area, surface charge, and other structural features varied, depending on the isolation methods. All three lignin fractions had negative surface charges, and ethanol-extracted lignin carried the highest surface charges, followed by dioxane-extracted lignin and cellulase-purified residual lignin. These physicochemical properties of lignin fractions also resulted in different extent of inhibitory effects on enzymatic hydrolysis of microcrystalline cellulose (MCC). Dioxane-extracted lignin exhibited the highest inhibitory effect on glucose release from MCC, followed by the cellulase-purified residual lignin fraction and ethanol-extracted lignin. Furthermore, lignin fractions with higher contents of syringyl (S) substructure and β-O-4 aryl ether interunit linkages showed a stronger negative effect on cellulase hydrolysis of MCC.
- Authors:
-
- Hubei Univ. of Technology, Wuhan (China). Hubei Provincial Key Lab. of Green Materials for Light Industry
- State Univ. of New York (SUNY), Syracuse, NY (United States). College of Environmental Science and Forestry, Dept. of Paper and Bioprocess Engineering
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
- Hubei Univ. of Technology, Wuhan (China). Key Lab. of Fermentation Engineering (Ministry of Education), College of Bioengineering
- Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering and Dept. of Forestry, Wildlife and Fisheries, Center for Renewable Carbon
- Hubei Univ. of Technology, Wuhan (China). Key Lab. of Fermentation Engineering (Ministry of Education), College of Bioengineering; Univ. of Tennessee, Knoxville, TN (United States). Dept. of Chemical and Biomolecular Engineering
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE; National Natural Science Foundation of China (NSFC); Chinese Scholarship Council; Ministry of Education of China; Hubei Provincial Department of Education; Foundation of Hubei
- OSTI Identifier:
- 1651274
- Grant/Contract Number:
- AC05-00OR22725; 21978074; 31871789; 2011842330; 201508420257; KF201611; KF201719; D20161402; 201611B01; 201806A02
- Resource Type:
- Accepted Manuscript
- Journal Name:
- BioResources
- Additional Journal Information:
- Journal Volume: 15; Journal Issue: 3; Journal ID: ISSN 1930-2126
- Publisher:
- NC State University
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; avicel; cellulase; corn stover; lignin isolation; physico-chemical properties
Citation Formats
Yang, Haitao, Zhang, Yuhang, Yoo, Chang Geun, Meng, Xianzhi, Chen, Xiong, Ragauskas, Arthur J., and Yao, Lan. Physico-chemical properties of lignin fractions from acid pretreated corn stover and their effects on enzymatic hydrolysis of microcrystalline cellulose. United States: N. p., 2020.
Web. doi:10.15376/biores.15.3.4898-4911.
Yang, Haitao, Zhang, Yuhang, Yoo, Chang Geun, Meng, Xianzhi, Chen, Xiong, Ragauskas, Arthur J., & Yao, Lan. Physico-chemical properties of lignin fractions from acid pretreated corn stover and their effects on enzymatic hydrolysis of microcrystalline cellulose. United States. https://doi.org/10.15376/biores.15.3.4898-4911
Yang, Haitao, Zhang, Yuhang, Yoo, Chang Geun, Meng, Xianzhi, Chen, Xiong, Ragauskas, Arthur J., and Yao, Lan. Mon .
"Physico-chemical properties of lignin fractions from acid pretreated corn stover and their effects on enzymatic hydrolysis of microcrystalline cellulose". United States. https://doi.org/10.15376/biores.15.3.4898-4911. https://www.osti.gov/servlets/purl/1651274.
@article{osti_1651274,
title = {Physico-chemical properties of lignin fractions from acid pretreated corn stover and their effects on enzymatic hydrolysis of microcrystalline cellulose},
author = {Yang, Haitao and Zhang, Yuhang and Yoo, Chang Geun and Meng, Xianzhi and Chen, Xiong and Ragauskas, Arthur J. and Yao, Lan},
abstractNote = {Lignin has been shown to be a recalcitrance factor in many biomass conversion studies. To better understand the effects of lignin on cellulose conversion, different lignin fractions were extracted from the same dilute acid pretreated corn stover by three sequential isolation methods, namely ethanol extraction, dioxane extraction, and enzyme purification. The physicochemical properties of each lignin fraction including molecular weight distribution, surface area, surface charge, and other structural features varied, depending on the isolation methods. All three lignin fractions had negative surface charges, and ethanol-extracted lignin carried the highest surface charges, followed by dioxane-extracted lignin and cellulase-purified residual lignin. These physicochemical properties of lignin fractions also resulted in different extent of inhibitory effects on enzymatic hydrolysis of microcrystalline cellulose (MCC). Dioxane-extracted lignin exhibited the highest inhibitory effect on glucose release from MCC, followed by the cellulase-purified residual lignin fraction and ethanol-extracted lignin. Furthermore, lignin fractions with higher contents of syringyl (S) substructure and β-O-4 aryl ether interunit linkages showed a stronger negative effect on cellulase hydrolysis of MCC.},
doi = {10.15376/biores.15.3.4898-4911},
journal = {BioResources},
number = 3,
volume = 15,
place = {United States},
year = {Mon May 11 00:00:00 EDT 2020},
month = {Mon May 11 00:00:00 EDT 2020}
}