Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes
Abstract
Economical production of algal commodities (food, feed, and fuels) requires reusing cultivation water to reduce operating costs. While some algae strains show growth inhibition in reused water, other strains appear un-affected. Reusing water to grow different strains (i.e., crop rotation) could potentially improve overall biomass production compared to water reuse with the same strain by reducing accumulation of strain-specific waste products, limiting carryover of population-specific pathogens such as viruses, and introducing bacteria that degrade residual organic matter. Here, batch culture experiments tested the effects of reused cultivation water from the self-inhibiting diatom Staurosira sp. C323 on the diatom Navicula sp. SFP and green alga Chlorella sp. D046, as well as their microbiomes. Navicula sp. and Chlorella sp. grew well in Staurosira sp. reused water, indicating that previously observed self-inhibition was likely specific to this Staurosira strain. While algal mi-crobiomes were not significantly different between controls and reused water treatments, specific bacteria taxa were differentially abundant in reused treatments, suggesting that some taxa responded to compounds re-maining in the reused water. Finally, results suggest that algae growth responses in reused water may depend largely on strain-specific factors, and that crop rotation can support biomass production.
- Publication Date:
- Research Org.:
- Duke Univ., Durham, NC (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; National Science Foundation (NSF); Gordon and Betty Moore Foundation
- OSTI Identifier:
- 1673383
- Alternate Identifier(s):
- OSTI ID: 1664465
- Grant/Contract Number:
- EE0007091; EE0008518; 1644868; GBMF3768
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Algal Research
- Additional Journal Information:
- Journal Volume: 51; Journal ID: ISSN 2211-9264
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Algae cultivation; water reuse; algal biotechnology; marine microalgae; algae microbiome; crop rotation
Citation Formats
Loftus, Sarah E., Hunt, Dana E., and Johnson, Zackary I. Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes. United States: N. p., 2020.
Web. doi:10.1016/j.algal.2020.102067.
Loftus, Sarah E., Hunt, Dana E., & Johnson, Zackary I. Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes. United States. https://doi.org/10.1016/j.algal.2020.102067
Loftus, Sarah E., Hunt, Dana E., and Johnson, Zackary I. Fri .
"Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes". United States. https://doi.org/10.1016/j.algal.2020.102067. https://www.osti.gov/servlets/purl/1673383.
@article{osti_1673383,
title = {Reused cultivation water from a self-inhibiting alga does not inhibit other algae but alters their microbiomes},
author = {Loftus, Sarah E. and Hunt, Dana E. and Johnson, Zackary I.},
abstractNote = {Economical production of algal commodities (food, feed, and fuels) requires reusing cultivation water to reduce operating costs. While some algae strains show growth inhibition in reused water, other strains appear un-affected. Reusing water to grow different strains (i.e., crop rotation) could potentially improve overall biomass production compared to water reuse with the same strain by reducing accumulation of strain-specific waste products, limiting carryover of population-specific pathogens such as viruses, and introducing bacteria that degrade residual organic matter. Here, batch culture experiments tested the effects of reused cultivation water from the self-inhibiting diatom Staurosira sp. C323 on the diatom Navicula sp. SFP and green alga Chlorella sp. D046, as well as their microbiomes. Navicula sp. and Chlorella sp. grew well in Staurosira sp. reused water, indicating that previously observed self-inhibition was likely specific to this Staurosira strain. While algal mi-crobiomes were not significantly different between controls and reused water treatments, specific bacteria taxa were differentially abundant in reused treatments, suggesting that some taxa responded to compounds re-maining in the reused water. Finally, results suggest that algae growth responses in reused water may depend largely on strain-specific factors, and that crop rotation can support biomass production.},
doi = {10.1016/j.algal.2020.102067},
journal = {Algal Research},
number = ,
volume = 51,
place = {United States},
year = {Fri Sep 18 00:00:00 EDT 2020},
month = {Fri Sep 18 00:00:00 EDT 2020}
}
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