Reused Cultivation Water Accumulates Dissolved Organic Carbon and Uniquely Influences Different Marine Microalgae
Abstract
Reusing growth medium (water supplemented with nutrients) for microalgae cultivation is required for economical and environmentally sustainable production of algae bioproducts (fuels, feed, and food). However, reused medium often contains microbes and dissolved organic matter that may affect algae growth. While the accumulation of dissolved organic carbon (DOC) in reused medium has been demonstrated, it is unclear whether DOC concentrations affect algae growth or subsequent rates of algal DOC release. To address these questions, lab-scale experiments were conducted with three marine microalgae strains, Navicula sp. SFP, Staurosira sp. C323, and Chlorella sp. D046, grown in medium reused up to four times. Navicula sp. and Chlorella sp. grew similarly in reused medium as in fresh medium, while Staurosira sp. became completely inhibited in reused medium. Across the three algae, there was no broad trend between initial DOC concentration in reused medium and algae growth response. Navicula sp. released less DOC overall in reused medium than in fresh medium, but DOC release rates did not decrease proportionally with increased DOC concentrations. Net DOC accumulation was much lower than gross DOC released by Navicula sp. and Staurosira sp., indicating the majority of released DOC was degraded. Additionally, biodegradation experiments with reused media showedmore »
- Authors:
-
- Duke Univ., Durham, NC (United States)
- Publication Date:
- Research Org.:
- Duke Univ., Durham, NC (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE); National Science Foundation (NSF)
- OSTI Identifier:
- 1613411
- Grant/Contract Number:
- EE0007091; 1644868
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Bioengineering and Biotechnology
- Additional Journal Information:
- Journal Volume: 7; Journal ID: ISSN 2296-4185
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; biotechnology & applied microbiology; algae cultivation; water reuse; dissolved organic carbon; algae biotechnology; bacteria; marine microalgae; recalcitrance
Citation Formats
Loftus, Sarah E., and Johnson, Zackary I. Reused Cultivation Water Accumulates Dissolved Organic Carbon and Uniquely Influences Different Marine Microalgae. United States: N. p., 2019.
Web. doi:10.3389/fbioe.2019.00101.
Loftus, Sarah E., & Johnson, Zackary I. Reused Cultivation Water Accumulates Dissolved Organic Carbon and Uniquely Influences Different Marine Microalgae. United States. https://doi.org/10.3389/fbioe.2019.00101
Loftus, Sarah E., and Johnson, Zackary I. Tue .
"Reused Cultivation Water Accumulates Dissolved Organic Carbon and Uniquely Influences Different Marine Microalgae". United States. https://doi.org/10.3389/fbioe.2019.00101. https://www.osti.gov/servlets/purl/1613411.
@article{osti_1613411,
title = {Reused Cultivation Water Accumulates Dissolved Organic Carbon and Uniquely Influences Different Marine Microalgae},
author = {Loftus, Sarah E. and Johnson, Zackary I.},
abstractNote = {Reusing growth medium (water supplemented with nutrients) for microalgae cultivation is required for economical and environmentally sustainable production of algae bioproducts (fuels, feed, and food). However, reused medium often contains microbes and dissolved organic matter that may affect algae growth. While the accumulation of dissolved organic carbon (DOC) in reused medium has been demonstrated, it is unclear whether DOC concentrations affect algae growth or subsequent rates of algal DOC release. To address these questions, lab-scale experiments were conducted with three marine microalgae strains, Navicula sp. SFP, Staurosira sp. C323, and Chlorella sp. D046, grown in medium reused up to four times. Navicula sp. and Chlorella sp. grew similarly in reused medium as in fresh medium, while Staurosira sp. became completely inhibited in reused medium. Across the three algae, there was no broad trend between initial DOC concentration in reused medium and algae growth response. Navicula sp. released less DOC overall in reused medium than in fresh medium, but DOC release rates did not decrease proportionally with increased DOC concentrations. Net DOC accumulation was much lower than gross DOC released by Navicula sp. and Staurosira sp., indicating the majority of released DOC was degraded. Additionally, biodegradation experiments with reused media showed no further net decrease in DOC, suggesting the accumulated DOC was recalcitrant to the associated bacteria. Overall, these results suggest that taxa-specific factors may be responsible for algae growth response in reused medium, and that DOC release and accumulation are insensitive to prior cultivation rounds. Choosing an algae strain that is uninhibited by accumulated DOC is therefore critical to ensure successful water reuse in the algae industry.},
doi = {10.3389/fbioe.2019.00101},
journal = {Frontiers in Bioengineering and Biotechnology},
number = ,
volume = 7,
place = {United States},
year = {Tue May 14 00:00:00 EDT 2019},
month = {Tue May 14 00:00:00 EDT 2019}
}
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Water use and its recycling in microalgae cultivation for biofuel application
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The biology of biofouling diatoms and their role in the development of microbial slimes
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Implementation of UV-based advanced oxidation processes in algal medium recycling
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Closely related phytoplankton species produce similar suites of dissolved organic matter
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High Lipid Induction in Microalgae for Biodiesel Production
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Microalgae Reused Growth Medium Experiments Dataset
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The culture of Chlorella vulgaris in a recycled supernatant: Effects on biomass production and medium quality
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