Reciprocal Control of Hierarchical DNA Origami-Nanoparticle Assemblies

Johnson, Joshua A.; Dehankar, Abhilasha; Winter, Jessica O.; Castro, Carlos E.

doi:10.1021/acs.nanolett.9b02786

Title: Reciprocal Control of Hierarchical DNA Origami-Nanoparticle Assemblies

Full Record
Other Related Research

Abstract

DNA origami mechanisms offer promising tools for precision nanomanipulation of molecules or nanomaterials. Recent advances have extended the function of individual DNA origami devices to material scales via hierarchical assemblies. However, achieving rapid and precise control of large conformational changes in hierarchical assemblies remains a critical challenge. In this work, we demonstrate a method for controlling DNA origami-nanoparticle assemblies through a multiscale approach, in which nanoparticles impart control on the conformation of individual DNA origami mechanisms, whereas DNA origami assemblies control the conformation of nanoparticle arrays. Specifically, we show that the angular distributions of DNA origami hinge mechanisms are tunable as a function of nanoparticle size and distance from the hinge vertex. We selectively adjust the affinity of nanoparticle binding sites, resulting in hinge actuation via DNA melting without releasing the nanoparticle, thereby enabling rapid and reversible temperature-based actuation. Finally, we demonstrate this rapid actuation in DNA origami-nanoparticle arrays of length scales extending over a micron. These results provide guiding principles toward the design of dynamic, DNA-origami hierarchical materials capable of storing and releasing mechanical energy.

Authors:

Johnson, Joshua A. ^[1]; Dehankar, Abhilasha ^[1];

^[1];

^[1]

The Ohio State Univ., Columbus, OH (United States)

Publication Date:: Wed Oct 30 00:00:00 EDT 2019

Research Org.:: The Ohio State Univ., Columbus, OH (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Basic Energy Sciences (BES)

OSTI Identifier:: 1657521

Grant/Contract Number:: SC0017270

Resource Type:: Accepted Manuscript

Journal Name:: Nano Letters

Additional Journal Information:: Journal Volume: 19; Journal Issue: 12; Journal ID: ISSN 1530-6984

Publisher:: American Chemical Society

Country of Publication:: United States

Language:: English

Subject:: 77 NANOSCIENCE AND NANOTECHNOLOGY; DNA nanotechnology; DNA origami; rapid actuation; dynamic nanostructures; nanoparticle composites; hierarchical assemblies

Citation Formats


                    Johnson, Joshua A., Dehankar, Abhilasha, Winter, Jessica O., and Castro, Carlos E. Reciprocal Control of Hierarchical DNA Origami-Nanoparticle Assemblies.  United States: N. p., 2019. 
Web.  doi:10.1021/acs.nanolett.9b02786.

Copy to clipboard


                    Johnson, Joshua A., Dehankar, Abhilasha, Winter, Jessica O., & Castro, Carlos E. Reciprocal Control of Hierarchical DNA Origami-Nanoparticle Assemblies.  United States.  https://doi.org/10.1021/acs.nanolett.9b02786

Copy to clipboard


                    Johnson, Joshua A., Dehankar, Abhilasha, Winter, Jessica O., and Castro, Carlos E. Wed .  
"Reciprocal Control of Hierarchical DNA Origami-Nanoparticle Assemblies".  United States.  https://doi.org/10.1021/acs.nanolett.9b02786.  https://www.osti.gov/servlets/purl/1657521.

Copy to clipboard


                    
@article{osti_1657521,

  title        = {Reciprocal Control of Hierarchical DNA Origami-Nanoparticle Assemblies},

  author       = {Johnson, Joshua A. and Dehankar, Abhilasha and Winter, Jessica O. and Castro, Carlos E.},

  abstractNote = {DNA origami mechanisms offer promising tools for precision nanomanipulation of molecules or nanomaterials. Recent advances have extended the function of individual DNA origami devices to material scales via hierarchical assemblies. However, achieving rapid and precise control of large conformational changes in hierarchical assemblies remains a critical challenge. In this work, we demonstrate a method for controlling DNA origami-nanoparticle assemblies through a multiscale approach, in which nanoparticles impart control on the conformation of individual DNA origami mechanisms, whereas DNA origami assemblies control the conformation of nanoparticle arrays. Specifically, we show that the angular distributions of DNA origami hinge mechanisms are tunable as a function of nanoparticle size and distance from the hinge vertex. We selectively adjust the affinity of nanoparticle binding sites, resulting in hinge actuation via DNA melting without releasing the nanoparticle, thereby enabling rapid and reversible temperature-based actuation. Finally, we demonstrate this rapid actuation in DNA origami-nanoparticle arrays of length scales extending over a micron. These results provide guiding principles toward the design of dynamic, DNA-origami hierarchical materials capable of storing and releasing mechanical energy.},

  doi          = {10.1021/acs.nanolett.9b02786},

  journal      = {Nano Letters},

  number       = 12,

  volume       = 19,

  place        = {United States},

  year         = {Wed Oct 30 00:00:00 EDT 2019},

  month        = {Wed Oct 30 00:00:00 EDT 2019}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1021/acs.nanolett.9b02786

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 16 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Similar Records in DOE PAGES and OSTI.GOV collections:

The path towards functional nanoparticle-DNA origami composites

Journal Article Johnson, Joshua A. ; Dehankar, Abhilasha ; Robbins, Ariel ; ... - Materials Science and Engineering. R, Reports

Nanoparticles (NPs) hold tremendous promise for diverse applications infields such as imaging, sensing, nanorobotics, and for optical and electronic materials. These applications often require precisely controlled interactions between multiple NPs or between NPs and other components. Hence, the organization of NPs into composite materials has been an active area of research. DNA origami nanotechnology offers a promising path forward with unparalleled control over complex nanoscale geometry and functionalization, programmed dynamic and mechanical properties, stimulus response to local or externally applied triggers, and capability of assembly into higher-order 1D, 2D, or 3D materials. Furthermore, DNA origami self-assembly is rapid and scalable,more »« less
Cited by 10
https://doi.org/10.1016/j.mser.2019.06.003

Full Text Available
Hierarchical Assembly of Plasmonic Nanostructures using Virus Capsid Scaffolds and DNA Origami Tiles

Journal Article Wang, Debin ; Capehart, Stacy L. ; Pal, Suchetan ; ... - ACS Nano

Building plasmonic nanostructures using biomolecules as scaffolds has shown great potential for attaining tunable light absorption and emission via precise spatial organization of optical species and antennae. Here we report bottom-up assembly of hierarchical plasmonic nanostructures using DNA origami tiles and MS2 virus capsids. These serve as programmable scaffolds that provide molecular level control over the distribution of fluorophores and nm-scale control over their distance from a gold nanoparticle antenna. While previous studies on DNA origami assembly of plasmonic nanostructures focused on the distance-dependent response of single fluorophores, these hybrid nanostructures enable us to investigate the plasmonic response of themore »« less
https://doi.org/10.1021/nn5015819
Hierarchical Assembly of Plasmonic Nanostructures using Virus Capsid Scaffolds on DNA Origami Tiles

Journal Article Wang, Debin ; Capehart, Stacy L. ; Pal, Suchetan ; ... - ACS Nano, 8(8):7896-7904

Plasmonic nanoarchitectures using biological scaffolds have shown the potential to attain controllable plasmonic fluorescence via precise spatial arrangement of fluorophores and plasmonic antennae. However, previous studies report a predominance of fluorescence quenching for small metal nanoparticles (less than ~60 nm) due to their small scattering cross-sections. In this work, we report the design and performance of fluorescent plasmonic structures composed of fluorophore-modified virus capsids and gold nanoparticles (AuNPs) assembled on DNA origami tiles. The virus capsid creates a scaffold for control over the three dimensional arrangement of the fluorophores, whereas the DNA origami tile provides precise control over the distancemore »« less
https://doi.org/10.1021/nn5015819
A quantitative model for a nanoscale switch accurately predicts thermal actuation behavior

Journal Article Crocker, Kyle ; Johnson, Joshua ; Pfeifer, Wolfgang ; ... - Nanoscale

Manipulation of temperature can be used to actuate DNA origami nano-hinges containing gold nanoparticles. We develop a physical model of this system that uses partition function analysis of the interaction between the nano-hinge and nanoparticle to predict the probability that the nano-hinge is open at a given temperature. The model agrees well with experimental data and predicts experimental conditions that allow the actuation temperature of the nano-hinge to be tuned over a range of temperatures from 30 °C to 45 °C. Additionally, the model identifies microscopic interactions that are important to the macroscopic behavior of the system, revealing surprising featuresmore »« less
https://doi.org/10.1039/d1nr02873a

Full Text Available
Construction of Reconfigurable and Polymorphic DNA Origami Assemblies with Coiled‐Coil Patches and Patterns

Journal Article Teng, Teng ; Bernal‐Chanchavac, Julio ; Stephanopoulos, Nicholas ; ... - Advanced Science

Abstract DNA origami nanodevices achieve programmable structure and tunable mechanical and dynamic properties by leveraging the sequence‐specific interactions of nucleic acids. Previous advances have also established DNA origami as a useful building block to make well‐defined micron‐scale structures through hierarchical self‐assembly, but these efforts have largely leveraged the structural features of DNA origami. The tunable dynamic and mechanical properties also provide an opportunity to make assemblies with adaptive structures and properties. Here the integration of DNA origami hinge nanodevices and coiled‐coil peptides are reported into hybrid reconfigurable assemblies. With the same dynamic device and peptide interaction, it is made multiplemore »« less
https://doi.org/10.1002/advs.202307257

Similar Records