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Title: Hierarchical Assembly of Plasmonic Nanostructures using Virus Capsid Scaffolds on DNA Origami Tiles

Abstract

Plasmonic nanoarchitectures using biological scaffolds have shown the potential to attain controllable plasmonic fluorescence via precise spatial arrangement of fluorophores and plasmonic antennae. However, previous studies report a predominance of fluorescence quenching for small metal nanoparticles (less than ~60 nm) due to their small scattering cross-sections. In this work, we report the design and performance of fluorescent plasmonic structures composed of fluorophore-modified virus capsids and gold nanoparticles (AuNPs) assembled on DNA origami tiles. The virus capsid creates a scaffold for control over the three dimensional arrangement of the fluorophores, whereas the DNA origami tile provides precise control over the distance between the capsid and the AuNP. Using finite-difference time-domain (FDTD) numerical simulations and multimodal single-particle imaging measurements, we show that the judicial design of these structures places the dye molecules near the hot spot of the AuNP. This effectively increases the fluorescence intensity in the quenching regime of the AuNP, with an enhancement factor that increases with increasing AuNP size. This strategy of using biological scaffolds to control fluorescence paves the way for exploring the parameters that determine plasmonic fluorescence. It may lead to a better understanding of the antenna effects of photon absorption and emission, enabling the construction ofmore » multicomponent plasmonic systems.« less

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1171891
Report Number(s):
PNNL-SA-98070
KC0302060
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
ACS Nano, 8(8):7896-7904
Additional Journal Information:
Journal Name: ACS Nano, 8(8):7896-7904
Country of Publication:
United States
Language:
English
Subject:
DNA origami; plasmonics; virus capsids

Citation Formats

Wang, Debin, Capehart, Stacy L., Pal, Suchetan, Liu, Minghui, Zhang, Lei, Schuck, P. J., Liu, Yan, Yan, Hao, Francis, Matthew B., and De Yoreo, James J. Hierarchical Assembly of Plasmonic Nanostructures using Virus Capsid Scaffolds on DNA Origami Tiles. United States: N. p., 2014. Web. doi:10.1021/nn5015819.
Wang, Debin, Capehart, Stacy L., Pal, Suchetan, Liu, Minghui, Zhang, Lei, Schuck, P. J., Liu, Yan, Yan, Hao, Francis, Matthew B., & De Yoreo, James J. Hierarchical Assembly of Plasmonic Nanostructures using Virus Capsid Scaffolds on DNA Origami Tiles. United States. doi:10.1021/nn5015819.
Wang, Debin, Capehart, Stacy L., Pal, Suchetan, Liu, Minghui, Zhang, Lei, Schuck, P. J., Liu, Yan, Yan, Hao, Francis, Matthew B., and De Yoreo, James J. Mon . "Hierarchical Assembly of Plasmonic Nanostructures using Virus Capsid Scaffolds on DNA Origami Tiles". United States. doi:10.1021/nn5015819.
@article{osti_1171891,
title = {Hierarchical Assembly of Plasmonic Nanostructures using Virus Capsid Scaffolds on DNA Origami Tiles},
author = {Wang, Debin and Capehart, Stacy L. and Pal, Suchetan and Liu, Minghui and Zhang, Lei and Schuck, P. J. and Liu, Yan and Yan, Hao and Francis, Matthew B. and De Yoreo, James J.},
abstractNote = {Plasmonic nanoarchitectures using biological scaffolds have shown the potential to attain controllable plasmonic fluorescence via precise spatial arrangement of fluorophores and plasmonic antennae. However, previous studies report a predominance of fluorescence quenching for small metal nanoparticles (less than ~60 nm) due to their small scattering cross-sections. In this work, we report the design and performance of fluorescent plasmonic structures composed of fluorophore-modified virus capsids and gold nanoparticles (AuNPs) assembled on DNA origami tiles. The virus capsid creates a scaffold for control over the three dimensional arrangement of the fluorophores, whereas the DNA origami tile provides precise control over the distance between the capsid and the AuNP. Using finite-difference time-domain (FDTD) numerical simulations and multimodal single-particle imaging measurements, we show that the judicial design of these structures places the dye molecules near the hot spot of the AuNP. This effectively increases the fluorescence intensity in the quenching regime of the AuNP, with an enhancement factor that increases with increasing AuNP size. This strategy of using biological scaffolds to control fluorescence paves the way for exploring the parameters that determine plasmonic fluorescence. It may lead to a better understanding of the antenna effects of photon absorption and emission, enabling the construction of multicomponent plasmonic systems.},
doi = {10.1021/nn5015819},
journal = {ACS Nano, 8(8):7896-7904},
number = ,
volume = ,
place = {United States},
year = {2014},
month = {7}
}